Prevalence of Helicobacter pylori cagA, babA2, and dupA genotypes andcorrelation with clinical outcome in Malaysian patients with dyspepsia

Abstract

Background/aim: The severity of disease outcome in dyspepsia has been attributed to Helicobacter pylori virulence genes. The aim of this study was to determine the distribution of H. pylori virulence genes (cagA, babA2, and dupA) and to determine whether or not there arises a significant correlation with clinical dyspepsia outcomes.Materials and methods: H. pylori genotypes cagA, babA2, and dupA were identified by polymerase chain reactions from gastric biopsy samples in 105 H. pylori-positive patients. Results:The positive rates for cagA, babA2, and dupA genes in H. pylori dyspeptic patients were 69.5%, 41.0%, and 22.9%, respectively. cagA was more prevalent in Indians (39.7%), babA2 was more prevalent in Malays (39.5%), and dupA detection occurred more frequently in both Indians and Malays and at the same rate (37.5%). The Chinese inhabitants had the lowest prevalence of the three genes. Nonulcer disease patients had a significantly higher distribution of cagA (76.7%), babA2 (74.4%), and dupA (75.0%). There was no apparent association between these virulence genes and the clinical outcomes. Conclusion:The lower prevalence of these genes and variations among different ethnicities implies that the strains are geographically and ethnically dependent. None of the virulence genes were knowingly beneficial in predicting the clinical outcome of H. pylori infection in our subjects.

Novel virulence factor dupA of Helicobacter pylori as an important risk determinant for disease manifestation: An overview

“…Another study in ethnic groups (Indian, Chinese and Malaya) of Malaysia reported that the prevalence of dupA was 22.9% in patients, which was in line with previous data (21.3%) conducted in Malaysia by Schmidt et al[ 41 ] (2009). In the later study, there was no association between dupA and clinical outcome[ 42 ].…”

Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales

“…Esta prevalencia es muy similar a lo encontrado en Tailandia, donde 96,7% de los infectados por el patógeno presentan la cepa cagA-positiva, no obstante, y de manera interesante, en este país la incidencia por cáncer gástrico no supera los 5/100.000 hab 21 . Otros estudios recientes muestran una prevalencia de 69,5%, 77,3% y 87,5%, para las cepas cagA-positivas en poblaciones de Malasia, India y China, respectivamente [22][23][24] . Por su parte, en América Latina, se reportan similares resultados para este tipo de cepas, con prevalencias de 62,6%, 71,0% y 85,6%, para México, Argentina y Brasil, respectivamente [25][26][27] .…”

Clinical and Endoscopic Features in Helicobacter Pylori Infection: Literature Review

“…9 The clinical outcomes caused by these bacteria depend on the host's genetic factors, virulence factors in the H. pylori strain, and environmental factors. 10,11 Identification of these bacteria is needed so that treatment can be given as soon as possible. 7 The diagnostic test options available for H. pylori infection are divided into invasive tests that include endoscopy, histology, culture, rapid urease test, and non-invasive tests, such as urea breath test, stool antigen sets, and serologic.…”