Prevalence of Helicobacter pylori in asymptomatic subjects—A nested PCR based study

Mishra, Sarita; Singh, Varsha; Rao, G. R. K.; Dixit, Vinod Kumar; Gulati, Anil Kumar; Nath, Gopal

doi:10.1016/j.meegid.2008.08.001

Cited by 44 publications

(36 citation statements)

References 10 publications

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“…The stool Ag positivity of H. pylori was related with age, increasing with age up to 41 -50 years category and then decreasing. Helicobacter pylori is typically acquired in early childhood in developing countries and usually persist during lifetime if left untreated (29), however, the H. pylori infection rate increases with age in adults (32). Although, serum assaying of anti-H. pylori IgG or IgA antibodies could be used to determine prevalence of infection (33), stool antigen test, as a non-invasive test, could be used both safely and cost effectively to screen patients (34,35).…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

Prevalence of Helicobacter pylori in Southern Part of Iran

Niknam¹,

Fattahi²,

Sepehrimanesh³

et al. 2018

Jundishapur J Microbiol

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Background: There are clear variations between and within countries regarding the prevalence of Helicobacter pylori. In addition, there are no estimations of its prevalence in the general population in the south of Iran. Objectives: We aimed to evaluate the prevalence of H. pylori in the rural population of Kavar, a southern city of Iran. Methods: A random sample of 500 individuals were selected from the Kavar cohort study. Serum total IgG against H. pylori was checked. The serum positive patients recalled for stool sampling and the H. pylori stool antigen was checked using sandwich ELISA. Moreover, age and gender were recorded for all patients. Results: Among the remained 441 who participated, 254 patients (57.6%) were positive for serum IgG. A total of 14 patients (5.5%) gave up due to stool sampling unsatisfaction. Overall, the rate of H. pylori infection based on both serum IgG and stool Ag of H.

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Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

Prevalence of Helicobacter pylori in Southern Part of Iran

Niknam¹,

Fattahi²,

Sepehrimanesh³

et al. 2018

Jundishapur J Microbiol

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“…However, H. pylori is disappearing from populations in developed countries [1,2]. In developing countries, up to 90% of the adult population carries the organism [3,4]. H. pylori is acquired early in life [5,6] and generally persists unless hosts are treated with antibiotics [1].…”

Section: Introductionmentioning

confidence: 99%

Serologic host response to Helicobacter pylori and Campylobacter jejuni in socially housed Rhesus macaques (Macaca mulatta)

et al. 2012

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BackgroundHelicobacter pylori are successful colonizers of the human gastric mucosa. Colonization increases the risk of peptic ulcer disease and adenocarcinoma. However, potential benefits of H. pylori colonization include protection against early-onset asthma and against gastrointestinal infections. Campylobacter jejuni are a leading cause of bacterial diarrhea and complications include Guillain-Barré syndrome. Here, we describe the development of reliable serological assays to detect antibodies against those two bacteria in Rhesus macaques and investigated their distribution within a social group of monkeys.MethodsTwo cohorts of monkeys were analyzed. The first cohort consisted of 30 monkeys and was used to establish an enzyme-linked immunosorbent assay (ELISA) for H. pylori antibodies detection. To evaluate colonization of those macaques, stomach biopsies were collected and analyzed for the presence of H. pylori by histology and culture. C. jejuni ELISAs were established using human serum with known C. jejuni antibody status. Next, plasma samples of the 89 macaques (Cohort 2) were assayed for antibodies and then statistically analyzed.ResultsAn H. pylori IgG ELISA, which was 100% specific and 93% sensitive, was established. In contrast, the IgA ELISA was only 82% specific and 61% sensitive. The CagA IgG assay was 100% sensitive and 61% of the macaques were positive. In cohort 2, 62% macaques were H. pylori sero-positive and 52% were CagA positive. The prevalence of H. pylori IgG and CagA IgG increased with monkey age as described for humans. Of the 89 macaques 52% showed IgG against C. jejuni but in contrast to H. pylori, the sero-prevalence was not associated with increasing age. However, there was a drop in the IgG (but not in IgA) mean values between infant and juvenile macaques, similar to trends described in humans.ConclusionsRhesus macaques have widespread exposure to H. pylori and C. jejuni, reflecting their social conditions and implying that Rhesus macaques might provide a model to study effects of these two important human mucosal bacteria on a population.

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“…Recent Indian data show a significant decline in the background prevalence of H. pylori with about 45% asymptomatic subjects harboring infection [26]. This downward trend in prevalence is significant because a small decline in the community prevalence of H. pylori infection markedly increases the proportion of H. pylorinegative ulcers compared to H. pylori-positive ulcers, if the total prevalence of peptic ulcer disease in the community remains unchanged.…”

Section: Discussionmentioning

confidence: 99%