“…The HEVs associated with human hepatitis are classified into four genotypes (Lu, Li & Hagedorn, 2006). Genotypes 1 and 2 cause waterborne outbreaks in developing countries and genotypes 3 and 4 are considered to be zoonotic and transmitted through the consumption of uncooked or undercooked contaminated meat (Mitsui et al, 2004;Wong, Purcell, Sreenivasan, Prasad, & Pavri, 1980). Total RNA was extracted from sludge suspensions using QIAamp Viral RNA Kits (QIAGEN, Hilden, Germany).…”