“…In Europe, Asia, and Northern American continents, HEV3 subtypes from 3a to 3 m and HEV4 from 4a to 4 g were widely detected (Smith et al, 2020 ) with special regard to the 3f (Abravanel et al, 2020 ; Schemmerer et al, 2022 ). Focusing on the European scientific literature, HEV3c, 3e, and 3f subtypes were mainly discovered, with special regard to the domestic pig ( Sus scrofa domesticus ) which represents the main HEV reservoir in the so-called “ domestic viral life-cycle ” (Di Bartolo et al, 2017 ; Ivanova et al, 2015 ), and wild boars ( Sus scrofa ) resulted to be involved in the “ wild one .” Furthermore, due to the increasing anthropization and urbanization processes, the environmental sharing with wild animal species (wild boars and ruminants) provides epidemiological and ecological conditions for possible cross-species infections (Anheyer-Behmenburg et al, 2017 ; Casares-Jimenez et al, 2021 ; Chandra et al, 2008 ; Wu et al, 2022 ). Basing on the viral trophism for hepatocytes, and secondly to the viremia step, and myosatellite cells, the biomolecular assays on these mentioned tissues result necessary for further public sanitary considerations (Aspinall et al, 2017 ).…”