Prevalence of Herpesvirus 8 Infection in Type 2 Diabetes Mellitus Patients

Ingianni, Angela; Carta, Francesca; Reina, Anna; Manai, Mario; Desogus, A; Pompei, Raffaello

doi:10.3844/ajidsp.2007.123.127

Cited by 13 publications

(16 citation statements)

References 16 publications

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“…A) showed that 17% (19/108) of healthy donors were HHV8‐positive, confirming previous data on HHV8 general prevalence in Sardinian population [Ingianni et al, ]. By contrast, 96/168 (57%) of type 2 diabetes patients resulted infected with HHV8, confirming the high prevalence of HHV8 infection in Sardinian patients affected by type 2 diabetes [Ingianni et al, ].…”

Section: Resultssupporting

confidence: 83%

“…The susceptibility to type 2 diabetes is thought to be related to both genetic and environmental factors, possibly including infection by chronic stressors for immune system such as herpesviruses. In accord with this hypothesis, we and others observed an increased frequency of human herpesvirus 8 (HHV8) infection in type 2 diabetes patients, respectively in the Sardinian population [Ingianni et al, ] and in the sub‐Saharan Africans [Sobngwi et al, ].…”

Section: Introductionsupporting

confidence: 74%

“…In particular, preexisting chronic infections have been associated to increased risk of developing type 2 diabetes, including infections with hepatitis C virus (HCV) [Lecube et al, ; Granados‐Montiel et al, ], herpes simplex 1 (HSV‐1) [Sun et al, ], human cytomegalovirus (CMV) [Chen et al, ], and HHV8 [Ingianni et al, ; Sobngwi et al, ].…”

Section: Discussionmentioning

confidence: 99%

“…Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood by Lymphoprep technique (Nycomed Pharma AS, Oslo, Norway). Total cellular DNA was extracted (Easy‐DNA, Invitrogen, San Diego, CA) and analyzed by PCR amplification for HHV8 ORF26 gene, as already described [Ingianni et al, ]. The amplification products were detected by ethidium bromide–stained agarose gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

“…The amplification products were detected by ethidium bromide–stained agarose gel electrophoresis. The PCR product specificity was confirmed by Southern blot assay, and the integrity and efficiency of the extracted DNA was checked by amplification of the human β‐globulin gene [Ingianni et al, ].…”

Section: Methodsmentioning

confidence: 99%

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High prevalence of HHV8 infection and specific killer cell immunoglobulin-like receptors allotypes in Sardinian patients with type 2 diabetes mellitus

et al. 2013

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The development of type 2 diabetes is thought to involve both environmental, possibly infectious, and genetic factors. Recently, a high prevalence of human herpesvirus 8 (HHV8) infection was observed in type 2 diabetes patients, and specific killer cell immunoglobulin-like receptors (KIR) allotypes were associated to both increased susceptibility to herpesvirus infection and risk to develop diabetes. However, no clear gene-disease or virus-disease associations have been established. To investigate the possible interplay between HHV8 infection, KIR allotype and type 2 diabetes, virus prevalence and KIR genotype were analyzed by PCR in 168 patients affected by type 2 diabetes and 108 control individuals belonging to the Sardinian population. Results showed a significant increase of HHV8 prevalence in type 2 diabetes patients versus controls (57% vs. 17%, P < 0.001), and a significant increase of KIR2DL2/DS2 homozygosity in diabetes patients infected with HHV8 compared to uninfected ones (64% vs. 14%, P < 0.0001), resulting in a significant OR of 11.31. In addition, the analysis of the frequency of the KIR2DL2/DS2 receptor and its HLA-C1 ligand, accordingly to the status of HHV8 infection, showed a significant increased correlation between KIR2DL2/DS2, type 2 diabetes and HLA-C1C1 genotype in the type 2 diabetes patients infected with HHV8 compared to uninfected ones (62% vs. 15%, P < 0.0001, OR = 8.64). These findings provide preliminary evidence that HHV8 infection might be a cofactor for type 2 diabetes in a specific subset of genetically susceptible individuals, and suggest the possibility that such patients might have an impaired immune-mediated component contributing to the development of type 2 diabetes.

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Section: Resultssupporting

confidence: 83%

Section: Introductionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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High prevalence of HHV8 infection and specific killer cell immunoglobulin-like receptors allotypes in Sardinian patients with type 2 diabetes mellitus

et al. 2013

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The Role of Human Herpesvirus 8 in Diabetes Mellitus Type 2: State of the Art and a Medical Hypothesis

Pompei

2015

Advances in Experimental Medicine and Biology

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Diabetes is a common chronic disease due to an altered glucose metabolism, caused by the quantitative and/or qualitative dysfunction of the insulin hormone. Two types of diabetes are recognized: juvenile diabetes, or type 1, which has an autoimmune origin, and adult diabetes, or type 2 (DMT2), which covers 90-95 % of all diabetic patients.The causes of DMT2 are not yet clear: heredity, life style, nutrition, and environment are considered the main risk factors. Several viral infections, namely cytomegalovirus, coxsackie and other enteroviruses, rubella and hepatitis C virus, have been claimed to be associated with some forms of diabetes. The direct role of viruses as a cause or as a risk of type 1 diabetes has been amply described in several recent reviews. Therefore, this review focuses attention on the role of a human herpes pathogenic virus in the onset of DMT2. By carrying out an analysis of recent literature, we describe the findings reported on an extremely deceitful virus, such as Human Herpes virus 8, and present a medical hypothesis on a possible relationship between this virus and DMT2.

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High Prevalence of Human Herpesvirus 8 Infection in Diabetes Type 2 Patients and Detection of a New Virus Subtype

Piras

Madeddu

Angius

et al. 2016

Advances in Experimental Medicine and Biology

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The prevalence of Human Herpesvirus 8 (HHV8) DNA and antiviral antibodies in Diabetes type 2 (DM2) and control subjects was studied, in order to confirm a possible link between DM2 and HHV8 infection. The HHV8-DNA from diabetic patients was typed for detecting possible genomic differences with known HHV8 reference viruses.DM2 patients and healthy controls were examined for the presence of HHV8 DNA into the peripheral blood lymphocytes. Both anti-lytic and latent phase antibodies were detected in HHV8 positive and negative diabetic patients, as well in a number of controls. The HHV8 ORF K1 and ORF 26 genes from DM2 patients were typed and matched to reference strains.A significant prevalence of HHV8 DNA in DM2 subjects versus healthy controls was detected (about 58 % against 27 %). Anti-lytic phase, but not anti-latent phase antibodies, were significantly increased in DM2 patients versus controls. In addition, about 30 % of HHV8 strains isolated from DM2 lymphocytes showed consistent differences in the ORF 26 gene sequence, so that a new HHV8 subtype was proposed. These findings give additional support to the hypothesis that HHV8 could be considered an additional risk factor for DM2 onset.

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Prevalence of Herpesvirus 8 Infection in Type 2 Diabetes Mellitus Patients

Cited by 13 publications

References 16 publications

High prevalence of HHV8 infection and specific killer cell immunoglobulin-like receptors allotypes in Sardinian patients with type 2 diabetes mellitus

High prevalence of HHV8 infection and specific killer cell immunoglobulin-like receptors allotypes in Sardinian patients with type 2 diabetes mellitus

The Role of Human Herpesvirus 8 in Diabetes Mellitus Type 2: State of the Art and a Medical Hypothesis

High Prevalence of Human Herpesvirus 8 Infection in Diabetes Type 2 Patients and Detection of a New Virus Subtype

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