Prevalence of human herpesvirus 8 infection in people living with HIV/AIDS in Pernambuco, Brazil

Cahú, Georgea Gertrudes de Oliveira Mendes; Morais, Viviane Martha Santos de; Lopes, Thaísa Regina Rocha; Silva, Dayvson Maurício da; Tozetto‐Mendoza, Tânia Regina; Pannuti, Cláudio Sérgio; Coêlho, Maria Rosângela Cunha Duarte

doi:10.1002/jmv.24550

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…The study population did not differ in age and sex when compared to other studies evaluating PLWHA infected with HHV-8 [4, 8, 31–33]. Similarly, the median HIV viral load and TCD4 count did not show a statistically significant difference between the HIV/HHV-8 coinfected and the HIV monoinfected patients, corroborating works that also evaluated these clinical characteristics [31, 32, 34].…”

Section: Discussionsupporting

confidence: 81%

Mannose-binding lectin concentrations in people living with HIV/AIDS infected by HHV-8

et al. 2019

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BackgroundMannose-binding lectin (MBL) plays an important role in the innate immune response by activating the complement system via the lectin pathway, and it has been studied in several viral infections; however, the influence of MBL in PLWHA infected with HHV-8 is unknown. The objective of this study was to verify the association of MBL deficient plasma concentrations in HIV/HHV-8 coinfected and HIV monoinfected patients and to correlate these concentrations with HIV viral load and CD4 counts in both groups.ResultsThis was an analytical study of case-controls consisting of PLWHA monitored at the medical outpatient of Infectious and Parasitic Diseases of the clinical hospital in the Federal University of Pernambuco. Plasma concentrations of MBL were obtained by an enzyme-linked immunosorbent assay (ELISA) using a commercial Human Mannose Binding Lectin kit (MyBioSource, Inc.) that was performed according to the manufacturer’s guidelines, with values < 100 ng/ml considered deficient. A total of 245 PLWHA samples were analysed; 118 were HIV/HHV-8 coinfected and 127 were HIV monoinfected; 5.1% (6/118) of the coinfected patients and 3.2% (4/127) of the monoinfected patients (p = 0.445) were considered plasma concentration deficient. The median of the plasma concentrations of MBL in the coinfected patients was 2803 log10 ng/ml and was 2.959 log10 ng/ml in the monoinfected patients (p = 0.001). There was an inverse correlation between the plasma concentrations of MBL and the HIV viral load in both groups, but no correlation with the CD4 count.ConclusionsAlthough the plasma concentrations considered deficient in MBL were not associated with HHV-8 infection in PLWHA, the coinfected patients showed lower MBL concentrations and an inverse correlation with HIV viral load, suggesting that there may be consumption and reduction of MBL due to opsonization of HIV and HHV-8, leading to the reduction of plasma MBL and non-accumulation in the circulation.

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Section: Discussionsupporting

confidence: 81%

Mannose-binding lectin concentrations in people living with HIV/AIDS infected by HHV-8

et al. 2019

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“…This is a cross section of PLWHA, with and without HHV-8 infection, in which patients’ demographic and laboratory variable data were originally described by Cahú et al [ 32 ] in a research on the HIV/HHV-8 coinfection prevalence in 500 PLWHA, reporting 143 HIV/HHV-8 coinfected and 357 HIV monoinfected. Of this total, we randomly selected 124 HIV/HHV-8 coinfected and 213 HIV monoinfected patients, by the statistical program Epi Info version 7.1.5 (CDC, Atlanta, GA, USA), who were followed up by the doctors from the outpatient clinic Infectious and Parasitic Diseases in the Clinical Hospital of the Federal University of Pernambuco.…”

Section: Methodsmentioning

confidence: 99%

MBL2 gene polymorphisms in HHV-8 infection in people living with HIV/AIDS

et al. 2018

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BackgroundHost genetic factors such as MBL2 gene polymorphisms cause defects in the polymerization of MBL protein and result in a functional deficiency and/or in low serum levels that can influence susceptibility to various viral infections. The aim of this study was to estimate the frequency of alleles, genotypes and haplotypes related to -550, -221 and exon 1 polymorphisms of the MBL2 gene and investigate their association with HHV-8 in people living with HIV/AIDS (PLWHA), as well as the impacts on CD4 cell count and HIV viral load in HIV/HHV-8 coinfected and HIV monoinfected patients.ResultsA cross sectional study in PLWHA, with and without HHV-8 infection, exploring associations between different factors, was performed in the outpatient infectious and parasitic diseases clinic at a referral hospital. Genomic DNA extractions from leukocytes were performed using a commercial Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The promoter region (-550 and -221) was genotyped with the TaqMan system (Applied TaqMan Biosystems® genotyping Assays), and the structural region (exon1) was genotyped with Express Sybr Greener Supermix kit (Invitrogen, USA). In total, 124 HIV/HHV-8 coinfected and 213 HIV monoinfected patients were analysed. Median TCD4 counts were significantly lower in HIV/HHV-8 coinfected patients, whereas the mean of the first and last viral load of HIV did not present significant difference. There was no difference in frequency between the LL, YY and AA genotypes between the HIV/HHV-8 coinfected or HIV monoinfected patients. However, in a multivariate analysis, coinfected patients with the intermediate expression haplotype of the MBL2 gene had an odds ratio of 3.1-fold (CI = 1.2–7.6) of their last CD4 cell count being below 350 cells/mm3. Among the coinfected individuals, four developed KS and presented the intermediate expression MBL haplotype, with three being HYA/LXA and one being LYA/LYO.ConclusionsHost genetic factors, such as -550, -221 and exon 1 polymorphisms, can be related to the may modify coinfections and/or to the development clinical manifestations caused by HHV-8, especially in HIV/HHV-8 coinfected patients who present the intermediate expression haplotypes of MBL.

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Human Herpesvirus 8 in Perinatally HIV-infected Children with Interstitial Lung Disease

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Fabro

Machado

et al. 2017

Journal of Tropical Pediatrics

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Prevalence of human herpesvirus 8 infection in people living with HIV/AIDS in Pernambuco, Brazil

Cited by 4 publications

References 17 publications

Mannose-binding lectin concentrations in people living with HIV/AIDS infected by HHV-8

Mannose-binding lectin concentrations in people living with HIV/AIDS infected by HHV-8

MBL2 gene polymorphisms in HHV-8 infection in people living with HIV/AIDS

Human Herpesvirus 8 in Perinatally HIV-infected Children with Interstitial Lung Disease

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