Prevalence of human papillomavirus, Epstein–Barr virus, p21, and p53 expression in sinonasal inverted papilloma, nasal polyp, and hypertrophied turbinate in Hong Kong patients

Sham, Cheuk Lun; To, Ka Fai; Chan, Paul K.S.; Lee, Dennis L.Y.; Tong, Michael C. F.; Hasselt, C. Andrew van

doi:10.1002/hed.21772

Cited by 72 publications

(69 citation statements)

References 81 publications

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“…Multiple studies have shown association between HPV infection and both the development and malignant transformation of sinonasal inverted papillomas. [50][51][52][53][54][55] Recently, an inverted papilloma of the lower female genital tract has been described with morphology similar to the inverted papilloma in the urinary tract. 56 This report, including only 2 cases, describes inverted papillomas occurring in the uterine cervix and the upper vagina in 60-and 50-year-old women, respectively.…”

Section: Discussionmentioning

confidence: 99%

Human Papillomavirus is Not an Etiologic Agent of Urothelial Inverted Papillomas

Alexander

Davidson

López-Beltrán

et al. 2013

American Journal of Surgical Pathology

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Inverted papilloma of the urinary bladder is rare, accounting for <1% of all bladder neoplasms. Although there is general consensus that inverted papilloma is benign in nature, little is known about its pathogenesis. Some have suggested that human papillomavirus (HPV) plays an etiologic role in the development of this neoplasm. These claims have not been adequately substantiated, and there is controversy as to the role of HPV in other urinary bladder neoplasms as well. To further investigate a possible etiologic role of HPV in urothelial neoplasia, we evaluated 27 inverted papillomas of the urinary bladder for the presence of HPV. Both immunohistochemical and in situ hybridization (ISH) studies for HPV and immunohistochemical analysis for p16, a surrogate marker for HPV infection, were used to assess HPV infection status. In the urinary bladder inverted papillomas of these 27 patients (age range, 35 to 78 y; M:F ratio, 11:1), no HPV was detected by HPV immunohistochemistry or by ISH. Immunoreactivity to p16 was detected in 11/27 (41%) of the cases. Expression of p16 is seen inconsistently within these neoplasms and does not correlate with the presence of HPV antigens or genes by immunohistochemistry or ISH, respectively. Therefore, p16 is not a reliable surrogate marker for HPV infection in urothelial inverted papilloma. Our findings indicate the absence of HPV in urothelial inverted papillomas. HPV testing should not be used as a diagnostic adjunct for inverted papilloma cases.

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Section: Discussionmentioning

confidence: 99%

Human Papillomavirus is Not an Etiologic Agent of Urothelial Inverted Papillomas

Alexander

Davidson

López-Beltrán

et al. 2013

American Journal of Surgical Pathology

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“…In another study on 13 nasal polyps, the same authors found EBV prevalence of 15, 69, and 85% using Southern blot hybridization, qualitative PCR, and in situ hybridization, respectively [18]; this study evidenced a highly different sensitivity with these different methods and led the author to hypothesize that nasal mucosa is a site where EBV persists through a low replicative level in resident lymphocytes. More recently, a 35% EBV positivity in 23 nasal polyps was found by qualitative PCR [19]; whereas in a study on nasal polyps and hypertrophied turbinates from Hong Kong patients, no specimen was positive to EBV in situ hybridization [20]. Taking into account also the different methods and particularly the absence of quantitative molecular data in previous studies, it could be argued that EBV positivity in polyps represents its presence in the inflammatory lymphoid tissue.…”

Section: Discussionmentioning

confidence: 99%

Detection of Herpesviruses 1-6 and Community-Acquired Respiratory Viruses in Patients with Chronic Rhinosinusitis with Nasal Polyposis

Costa¹,

Garzaro

Boggio

et al. 2014

Intervirology

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Objective: To evaluate the prevalence of human herpesviruses (HHV) 1-6 and community-acquired respiratory viruses (CARVs) in specimens from patients with nasal polyposis undergoing functional endoscopic sinus surgery (FESS) and investigate the potential clinical role. Methods: Viral occurrence was evaluated by molecular methods in polyp, turbinate mucosa, and pre- and postoperative scraping specimens from 35 consecutive patients at different time points in relation to FESS. Results: Overall, 21 patients (60%) were positive to at least one virus in at least one specimen; in particular, 12.1% of all specimens for HHV-6 (3/35 polyps, 11/31 turbinates, 1 presurgical scraping) and 10.5% for Epstein-Barr virus (EBV) (8/35 polyps, 3/31 turbinates, 1/29 pre- and 1/29 postsurgical scraping), followed by CMV and HSV-1 (both 1.6%; 1/35 polyps, 1/29 postsurgical scraping and 2/35 polyps, respectively). EBV positivity tended to be higher in polyps, as well as HHV-6 in adjacent healthy turbinate mucosa, although no significant association was found. Only one preoperative cytological specimen was positive to parainfluenza virus-1. Conclusion: No association between the development of nasal polyps, herpesviruses and CARVs seems to exist. However, the higher EBV frequency in polyps could suggest a causative role or persistence in the inflammatory lymphoid tissue.

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“…In cancer tissue, p21 expression is not always associated with p53 expression as p21 expression levels are low in tissues containing p53 harboring a functional mutation (20). The prognosis and aggressiveness of head and neck cancer were revealed to be associated with the expression of PTEN, but not with p53 and p21 (21,22). In the present study, PTEN and p21 expression were positively correlated with one another, and these results indicate that the trigger for the malignant transformation of IPs to SCCs may underlie the activation of the PI3K/Akt signaling pathway via the loss of PTEN and the subsequent degradation of the p53-p21 pathway.…”

Section: Discussionmentioning

confidence: 99%

Role of microRNA-296-3p in the malignant transformation of sinonasal inverted papilloma

et al. 2017

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Abstract. Inverted papilloma (IP) is a benign tumor occurring in the nasal cavity and paranasal sinuses. It is reported that 5-15% of IPs undergo malignant transformation into squamous cell carcinoma (SCC), and the role of microRNAs (miRNA/miR) in this process remains to be elucidated. In the present study, whole miRNA profiles using samples of IP and SCC were investigated, in order to detect the function of miRNA in the carcinogenesis of IP. Samples from IPs (n=5) and SCC lesions (n=5), which arose from IPs, were used for miRNA analysis. A total of 200 miRNAs exhibited a >2-fold differential expression between IP and SCC. miR-296-3p was markedly upregulated in SCC with a 23-fold difference. Computational analysis indicated that miR-296-3p targeted PTEN, which regulates the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway and PTEN is involved in the carcinogenesis of SCC. miR-296-3p directly regulated PTEN expression in head and neck cancer cells, with PTEN protein levels decreased in 4/19 the SCCs (21.0%), as compared with those in the IPs (76.4%). Positive p21 staining was observed in 64.7% of IPs; this was a significantly increased rate compared with that for SCCs (26.3%, P=0.0086). The results of the present study indicated that there were marked changes in the miRNA expression signature during the malignant transition. miR-296-3p may serve an important role in the malignant transformation of IPs via the regulation of PTEN, combined with the subsequent inhibition of the PI3K/Akt signaling pathway, and may be a novel agent for cancer prevention.

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Prevalence of human papillomavirus, Epstein–Barr virus, p21, and p53 expression in sinonasal inverted papilloma, nasal polyp, and hypertrophied turbinate in Hong Kong patients

Abstract: HPV prevalence of our IP is low. EBV is not present in IP. High p21 and low p53 expression in IP suggests a non-p53-dependent regulation pathway.

Cited by 72 publications

References 81 publications

Human Papillomavirus is Not an Etiologic Agent of Urothelial Inverted Papillomas

Human Papillomavirus is Not an Etiologic Agent of Urothelial Inverted Papillomas

Detection of Herpesviruses 1-6 and Community-Acquired Respiratory Viruses in Patients with Chronic Rhinosinusitis with Nasal Polyposis

Role of microRNA-296-3p in the malignant transformation of sinonasal inverted papilloma

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