Prevalence of <i>Bartonella</i> species DNA and antibodies in cats (<i>Felis catus</i>) submitted to a spay/neuter program in Rio de Janeiro, Brazil

Crissiuma, Ana Lucia; Favacho, Alexsandra Rodrigues de Mendonça; Gershony, Liza C.; Mendes-de-Almeida, Flavya; Gomes, Raphael; Mares-Guia, Angélica; Rozental, Tatiana; Barreira, Jairo Dias; Lemos, Elba Regina Sampaio de; Labarthe, Norma

doi:10.1016/j.jfms.2010.08.010

Cited by 15 publications

(20 citation statements)

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“…Most cats from an animal shelter in city of Vassouras, RJ were infected (97.3% PCR-positive) (Souza et al 2010). Recently, B. henselae DNA and antibodies to Bartonella spp were detected in 42.5% and 47.5% of cats, respectively, undergoing neutering or spaying in RJ (Crissiuma et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a seroprevalence for Bartonella spp of 35.7% was observed among cats belonging to human immunodeficiency virus (HIV) antibody-positive individuals . In addition, Bartonella spp have been molecularly detected in cats from the states of Rio Grande do Sul (RS) (Staggemeier et al 2010) and Rio de Janeiro (RJ) (Souza et al 2010, Crissiuma et al 2011). There are few studies addressing the occurrence of Bartonella spp in humans in Brazil.…”

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confidence: 99%

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Molecular characterisation of Bartonella species in cats from São Luís, state of Maranhão, north-eastern Brazil

Braga

Diniz

André

et al. 2012

Mem. Inst. Oswaldo Cruz

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Bartonella species are fastidious Gram-negative bacteria that predominantly infect mammalian erythrocytes and endothelial cells and cause long-lasting bacteraemia in their reservoir hosts (Chomel et al. 2004). Bartonellae are usually vector-borne bacteria and the vector varies depending on the bacterial species, host and reservoir (Chomel et al. 2006b).Domestic cats are the main reservoir for Bartonella henselae (the most frequent agent of cat scratch disease), Bartonella clarridgeiae (suspected in a few cases of cat scratch disease) (Chomel et al. 2004) and Bartonella koehlerae (Avidor et al. 2004).There are few reports of Bartonella infection in Brazil. A low seroprevalence of Bartonella spp has been detected among Brazilian dogs (Diniz et al. 2007a, b) and B. henselae and Bartonella vinsonii berkhoffii DNA has been detected in Brazilian dogs at a very low prevalence (Diniz et al. 2007b). Conversely, a very high prevalence of antibodies to B. henselae has been observed among free-ranging wild felids in Brazil (Filoni et al. 2006) and the parasite has been detected in wild felids by molecular techniques (Guimarães et al. 2010). Recently, a seroprevalence for Bartonella spp of 35.7% was observed among cats belonging to human immunodeficiency virus (HIV) antibody-positive individuals . In addition, Bartonella spp have been molecularly detected in cats from the states of Rio Grande do Sul (RS) (Staggemeier et al. 2010) and Rio de Janeiro (RJ) (Souza et al. 2010, Crissiuma et al. 2011). There are few studies addressing the occurrence of Bartonella spp in humans in Brazil. Antibodies to B. henselae and Bartonella quintana have been detected in 13.7% and 12.8% of healthy residents of the state of Minas Gerais (MG), respectively (da Costa et al. 2005). A seroprevalence of Bartonella spp of 38.4% and 34.4% has been observed in HIV-positive patients and healthy human populations, respectively, in Jacarepaguá, RJ .This study aimed to detect and characterise Bartonella spp DNA from Brazilian cat blood samples from São Luís, Maranhão (MA), north-eastern Brazil. MATERIALS AND METHODSSample collection and study area -Between October 2008-January 2009, whole blood samples were collected from 200 domestic cats (102 males, 98 females) that were allowed outside in São Luís (194 cats from suburban areas of São Luís county and six from Raposa county). The study region is an invasion area that has suffered a 70% forest reduction. Cats were selected without specific inclusion criteria and were apparently healthy at the time of sample collection. Sampled cats had contact with other cats and dogs. Blood samples for polymerase chain reaction (PCR) were collected from jugular or cephalic veins, immediately aliquoted into tubes containing ethylenediamine tetraacetic acid (EDTA) as an anticoagulant and stored at -20ºC until PCR analysis. All procedures were performed according to Ethical Principles in Animal Research adopted by the Brazilian College of Animal Experimentation and were approved by the Ethical Committee of the College of Ag...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Molecular characterisation of Bartonella species in cats from São Luís, state of Maranhão, north-eastern Brazil

Braga

Diniz

André

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…Furthermore, Bartonella spp. DNA was detected in 17 out of 40 clinically healthy cats that were treated in a spaying/ neutering program in the city of Rio de Janeiro, state of Rio de Janeiro (CRISSIUMA et al, 2011). Recently, B. henselae DNA was detected in two out of 46 apparently healthy cats (4.3%) that were sampled in Jaboticabal, state of São Paulo (DE BORTOLI et al, 2012).…”

Section: Mycoplasma Haemofelismentioning

confidence: 99%

Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

Miceli

Gavioli

Gonçalves

et al. 2013

Rev. Bras. Parasitol. Vet.

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Hemotrophic mycoplasmas (hemoplasmas), Bartonella sp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas, Bartonella sp., Hepatozoon sp. and Cytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) for Mycoplasma haemofelis, 12 (6.7%) for 'Candidatus M. haemominutum' and one (0.5%) for 'Candidatus M. turicensis'. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, 'Candidatus M. haemominutum' and 'Candidatus M. turicensis', based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showed Cytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas and Bartonella species.

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“…Although in Brazil canine population is still bigger than cats, an increase in cat population has been noticed [12]. In Brazil, there is a wide variation in the prevalence of Bartonella spp DNA in domestic cats, ranging from 0.5% to 97.3% [13][14][15][16][17][18][19]. Hence, the aim of this study was to detect the presence of Bartonella spp.…”

Section: Introductionmentioning

confidence: 99%

Bartonella spp. and hematological changes in privately owned domestic cats from Rio de Janeiro, Brazil

Souza

Almosny

Favacho

et al. 2017

J Infect Dev Ctries

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Introduction: Bartonella infection in cats can represent a risk to owners, particularly today when considering the increase in cat populations and their role in human bartonellosis epidemiology. In the present study, we aimed to detect Bartonella spp. in blood samples from 163 asymptomatic privately-owned cats from the metropolitan area of Rio de Janeiro State, Brazil by using a conventional PCR test and also to evaluate the association between Bartonella spp. and hematological changes in positive cats. Methodology: PCR assays were performed targeting the Bartonella spp heat shock protein (htrA) gene and complete blood counts were also performed in all samples. Positive PCR samples were confirmed by the presence of two genes, citrate synthase (gltA) and RNA polymerase beta-subunit-encoding (rpoB). Results: A total of 74.85% (122/163) of the tested cats were positive for Bartonella spp and partial sequencing confirmed to be B. henselae. All hematological findings from the 163 cats tested (PCR-positive and negative), presented normal limits. Conclusions: This study demonstrates that B. henselae is present in almost 75% asymptomatic privately-owned domestic cats in the metropolitan region of Rio de Janeiro State, Brazil. Our results also show that hematological findings in Bartonella spp. infected cats are uncommon. In this scenario, the use of PCR as a diagnostic tool in feline Bartonella infections should be considered. Finally, these results also demonstrate the potential risk of Bartonella spp. infection in the human population of the metropolitan area of Rio de Janeiro State, Brazil.

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Prevalence of Bartonella species DNA and antibodies in cats (Felis catus) submitted to a spay/neuter program in Rio de Janeiro, Brazil

Cited by 15 publications

References 14 publications

Molecular characterisation of Bartonella species in cats from São Luís, state of Maranhão, north-eastern Brazil

Molecular characterisation of Bartonella species in cats from São Luís, state of Maranhão, north-eastern Brazil

Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

Bartonella spp. and hematological changes in privately owned domestic cats from Rio de Janeiro, Brazil

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