Prevalence of <i>blaZ</i> Gene and Performance of Phenotypic Tests to Detect Penicillinase in <i>Staphylococcus aureus</i> Isolates from Japan

Takayama, Yoshiko; Tanaka, Takashi; Oikawa, Koko; Fukano, Naoki; Goto, Mieko; Takahashi, Takashi

doi:10.3343/alm.2018.38.2.155

Cited by 23 publications

(13 citation statements)

References 11 publications

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“…The presence of the bla Z gene did not influence production of β-lactamase alone [ 50 , 51 ]. Here, not all penicillin-resistant S. aureus isolates from food (45.5%) and clinical samples (52.8%) possessed bla Z gene.…”

Section: Main Textmentioning

confidence: 99%

Emerging of antimicrobial resistance in staphylococci isolated from clinical and food samples in Algeria

et al. 2018

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ObjectiveThe antimicrobial resistance of staphylococci rose worldwide. In total, 96 Staphylococcus isolates from food and clinical samples were collected from two provinces in Algeria. The antimicrobial susceptibility testing was performed and resistance-associated genes were detected.ResultsFifty-one strains were isolated from food samples and differentiated into 33 Staphylococcus aureus and 18 coagulase-negative staphylococci. Forty-five staphylococci were collected from hospital and community-acquired infection cases. All S. aureus isolated from food were resistant to penicillin and 45.5% were resistant to tetracycline. The resistance rates of 45 clinical Staphylococcus isolates were 86.7%, 48.9%, 37.8% and 20.0% to penicillin, tetracycline, erythromycin and kanamycin, respectively. Nine isolates were confirmed as MRSA from food and clinical isolates. One S. aureus originated from food was confirmed as vancomycin-resistant. Multidrug-resistance was observed among 25.5% and 53.3% of food and clinical staphylococci, respectively. The tetM/K, blaZ, aacA-aphD, ermC and mecA genes were detected in food and clinical isolates. ermA gene was not found. This study provided insight into the status of antimicrobial resistance of staphylococci isolated from food and clinical samples in Algeria. Further investigations and surveillance programmes are mandatory.

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Section: Main Textmentioning

confidence: 99%

Emerging of antimicrobial resistance in staphylococci isolated from clinical and food samples in Algeria

et al. 2018

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“…When observing antimicrobial resistance in a clinical scenario metagenomic sequencing and assembly of whole samples may not be desired due to cost or time constraints. Instead, it may be more interesting to narrow down the resistance to a few potential candidate plasmids responsible for the resistance, then to either validate it experimentally with PCR or adjust treatment ( 36 ). For demonstration purposes, we investigate a resistance qualification in Staphylococcus aureus , which is infamous for e.g., methicillin resistance ( 37 ).…”

Section: Resultsmentioning

confidence: 99%

PLSDB: advancing a comprehensive database of bacterial plasmids

Schmartz

Hartung

Hirsch

et al. 2021

Nucleic Acids Research

146

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Plasmids are known to contain genes encoding for virulence factors and antibiotic resistance mechanisms. Their relevance in metagenomic data processing is steadily growing. However, with the increasing popularity and scale of metagenomics experiments, the number of reported plasmids is rapidly growing as well, amassing a considerable number of false positives due to undetected misassembles. Here, our previously published database PLSDB provides a reliable resource for researchers to quickly compare their sequences against selected and annotated previous findings. Within two years, the size of this resource has more than doubled from the initial 13,789 to now 34,513 entries over the course of eight regular data updates. For this update, we aggregated community feedback for major changes to the database featuring new analysis functionality as well as performance, quality, and accessibility improvements. New filtering steps, annotations, and preprocessing of existing records improve the quality of the provided data. Additionally, new features implemented in the web-server ease user interaction and allow for a deeper understanding of custom uploaded sequences, by visualizing similarity information. Lastly, an application programming interface was implemented along with a python library, to allow remote database queries in automated workflows. The latest release of PLSDB is freely accessible under https://www.ccb.uni-saarland.de/plsdb.

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“…The results from the current study did not find TEM , while CTXM2 and SHV1 were found in low levels, and CTXM1 was found in 16.7% of samples. Another gene related to β-lactamases was blaZ6 , which was present in only one sample, although the frequency of this gene varies depending on the region; for example, in Japan, it has a frequency between 1% and 3% [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Importance of Microbiome of Fecal Samples Obtained from Adolescents with Different Weight Conditions on Resistance Gene Transfer

et al. 2022

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Antimicrobial resistance (AMR) is a relevant public health problem worldwide, and microbiome bacteria may contribute to the horizontal gene transfer associated with antimicrobial resistance. The microbiome of fecal samples from Mexican adolescents were analyzed and correlated with eating habits, and the presence of AMR genes on bacteria in the microbiome was evaluated. Fecal samples from adolescents were collected and processed to extract genomic DNA. An Illumina HiSeq 1500 system was used to determine resistance genes and the microbiome of adolescents through the amplification of gene resistance and the V3–V4 regions of RNA, respectively. Analysis of the microbiome from fecal samples taken from 18 obese, overweight, and normal-weight adolescents revealed that the Firmicutes was the most frequent phylum, followed by Bacteroidetes, Actinobacteria, Proteobacteria and Verrucomicrobia. The following species were detected as the most frequent in the samples: F. prausnitzii, P. cori, B. adolescentis, E. coli and A. muciniphila. The presence of Bacteroides, Prevotella and Ruminococcus was used to establish the enterotype; enterotype 1 was more common in women and enterotype 2 was more common in men. Twenty-nine AMR genes were found for β-lactamases, fluoroquinolones, aminoglycosides, macrolide, lincosamides, streptogramin (MLS), tetracyclines and sulfonamides. The presence of microorganisms in fecal samples that harbor AMR genes that work against antimicrobials frequently used for the treatment of microbial infections such as b-lactams, macrolides, aminoglycosides, MLS, and tetracyclines is of great concern, as these organisms may be an important reservoir for horizontal AMR gene transfer.

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Prevalence of blaZ Gene and Performance of Phenotypic Tests to Detect Penicillinase in Staphylococcus aureus Isolates from Japan

Cited by 23 publications

References 11 publications

Emerging of antimicrobial resistance in staphylococci isolated from clinical and food samples in Algeria

Emerging of antimicrobial resistance in staphylococci isolated from clinical and food samples in Algeria

PLSDB: advancing a comprehensive database of bacterial plasmids

Importance of Microbiome of Fecal Samples Obtained from Adolescents with Different Weight Conditions on Resistance Gene Transfer

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