Prevalence of<i>Ehrlichia canis</i>infection in thrombocytopenic dogs from Rio de Janeiro, Brazil

Macieira, Daniel de Barros; Messick, Joanne B.; Cerqueira, Aloysio de Mello Figueiredo; Freire, Isabel Maria Alexandre; Linhares, Guido Fontgalland Coelho; Almeida, Núbia Karla de Oliveira; Almosny, Nádia Regina Pereira

doi:10.1111/j.1939-165x.2005.tb00008.x

Cited by 54 publications

(51 citation statements)

References 26 publications

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“…These results showed that a proportionally significant number of dogs infected by this pathogen were thrombocytopenic, indicating that this hematological alteration is an important clinical manifestation of CME. Similar results confirming the association of thrombocytopenia and infection by E. canis in dogs were described in Botucatu, State of Sao Paulo (BULLA et al, 2004) and Rio de Janeiro, State of Rio de Janeiro (MACIEIRA et al, 2005).…”

Section: Resultssupporting

confidence: 78%

“…These included 22% in Londrina, State of Parana, (DAGNONE et al, 2003), 35.6% in Salvador, State of Bahia , and 57% in Recife, State of Pernambuco (RAMOS et al, 2010 (DINIZ et al, 2007)]. Nevertheless, the prevalence level of this study was greater than those described in the city of Rio de Janeiro, State of Rio de Janeiro, 15%, (MACIEIRA et al, 2005) and two cities in the State of Bahia: Ilheus (10.7%; 9/84) and Itabuna (4.3%; 3/69) (CARVALHO et al, 2008). It must be emphasized that most of these studies were performed with dogs attended at Veterinary Hospitals, or with clinical manifestations and/or hematological alterations consistent with CME.…”

Section: Resultscontrasting

confidence: 53%

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Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

Silva

Benitez

Girotto

et al. 2012

Rev. Bras. Parasitol. Vet.

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Canine monocytic ehrlichiosis caused primarily by Ehrlichia canis and canine thrombocytic anaplasmosis induced by Anaplasma platys are important emerging zoonotic tick-borne diseases of dogs. There is evidence that these pathogens can also affect humans. This study evaluated the presence of E. canis and A. platys in blood samples collected from 256 domiciled dogs in the municipality of Jataizinho, located in north region of the State of Parana, Brazil, by PCR assay. The occurrence of E. canis and A. platys was 16.4% (42/256) and 19.4% (49/256), respectively; while 5.47% (14/256) of the dogs evaluated were co-infected by these two organisms. The presence of E. canis and A. platys was not significantly associated with the variables evaluated (sex, age, outdoor access, and presence of ticks during blood collection). Infection of dogs by E. canis was associated with anemia and thrombocytopenia, while infection induced by A. platys was related only to thrombocytopenia. Canine monocytic ehrlichiosis and canine thrombocytic anaplasmosis should be included in the differential diagnoses when these hematological alterations are observed during routine laboratory evaluation of dogs.Keywords: Canine monocytic ehrlichiosis, canine thrombocytic anaplasmosis, PCR, anemia, thrombocytopenia. ResumoErliquiose monocítica canina, causada principalmente por Ehrlichia canis, e anaplasmose trombocítica canina, devida a infecção com Anaplasma platys, são importantes doenças transmitidas por carrapatos que acometem os cães, com evidências que podem também acometer o homem. O presente estudo avaliou a ocorrência desses agentes em amostras de sangue de 256 cães domiciliados na cidade de Jataizinho, na região Norte do Paraná, Brasil, utilizando a técnica da Reação em Cadeia da Polimerase (PCR). A ocorrência de E. canis e A. platys foi de 16,4% (42/256) e 19,4% (49/256), respectivamente, com 5,47% (14/256) dos animais apresentando coinfecção. Não foi observada associação significativa com as variáveis sexo, idade, acesso à rua e presença de carrapatos no momento da coleta de sangue. A infecção por E. canis teve relação com anemia e com trombocitopenia, enquanto a infecção por A. platys apresentou relação apenas com trombocitopenia. Com base nos resultados obtidos, reforçou-se a necessidade de que erliquiose e anaplasmose canina devem estar entre os diagnósticos diferenciais, quando da detecção de anemia e trombocitopenia em exames laboratoriais.

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Section: Resultssupporting

confidence: 78%

Section: Resultscontrasting

confidence: 53%

Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

Silva

Benitez

Girotto

et al. 2012

Rev. Bras. Parasitol. Vet.

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“…The results for the city of Lavras (MG) is within the range reported by other studies conducted in Brazil, which have shown that between 14% and 45% of the dogs that are attended in veterinary hospitals and clinics are seropositive (18) . The frequency of E. canis in the serum of dogs in Lavras (MG) was lower than the results observed by Silva et al (19) in Cuiaba (MT), 42.5% (108/254; IFAT ≥40), and by Souza et al (20) in Salvador (BA), 35.6% (168/472; IFAT ≥80). However, Saito et al (21) observed a prevalence of 4.8% (19/389; IFAT ≥80) in Rio Grande do Sul, which was low compared to that obtained in the present study.…”

Section: Resultscontrasting

confidence: 50%

“…This information is helpful, but from a clinical perspective, it is more important to know how many dogs with anemia or thrombocytopenia have these diseases. Dogs infected with Brazilian strains of E. canis consistently develop anemia (35,36) , but they may (36) or may not develop thrombocytopenia (35) .…”

Section: Resultsmentioning

confidence: 99%

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

et al. 2017

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The aim of this study was to determine the prevalence of anti-Ehrlichia canis and anti-Babesia vogeli IgG antibodies in dogs and correlate this prevalence with risk factors to evaluate the relation of serological status to hematological findings. Blood samples of dogs attended from September 2011 to March 2012 at the veterinary hospital of the Federal University of Lavras, Brazil, were analyzed using an indirect fluorescence antibody test (IFAT). Of the total 160 dog serum samples, 23.7% (38 dogs; CI 95 17.7% -30.7%) were seropositive for E. canis, 40.0% (64 dogs; CI 95 40.0% -59.2%) for B. vogeli, and 5.6% (9 dogs) for both hemoparasites. None of the epidemiological variables showed a significant association (P>0.05) with seropositivity to E. canis and B. vogeli. Dogs seropositive for E. canis showed lower values for hematocrit (P<0.05). However, for the erythrogram, the platelet count, the leukogram and clinical signs, no significant difference (P>0.05) was observed between dogs that were seropositive and seronegative for E. canis or for B. vogeli. Serological results suggest that infection with E. canis and B. vogeli is endemic in the canine population in question, with a prevalence of the subclinical phase (asymptomatic) in dogs that are seropositive for ehrlichiosis or babesiosis. Keywords: babesiosis; canine; ehrlichiosis; IFAT; laboratory findings. ResumoO objetivo deste estudo foi determinar a prevalência de anticorpos IgG anti-Ehrlichia canis e antiBabesia vogeli em cães e correlacionar com a prevalência e fatores de risco para avaliar a relação do estado sorológico com os achados hematológicos. Amostras de sangue de cães coletadas de setembro de 2011 a março de 2012, no Hospital Veterinário da Universidade Federal de Lavras, Brasil, foram analisadas usando o teste de reação de imunofluorescência indireta (RIFI). Do total de amostras de soro de 160 cães, 23,7% (38 cães; IC95 17,7% -30,7%) foram soropositivos para E. canis, 40,0% (64 cães; IC95 40,0% -59,2%) para B. vogeli, e 5,6% (9 cães) para os dois hemoparasitas. Nenhuma das variáveis epidemiológicas mostrou associação significativa (P> 0,05) com a soropositividade para E. canis e B. vogeli. Cães soropositivos para E. canis mostraram valores médios mais baixos para hematócrito (P<0,05). No entanto, para o eritrograma, a contagem de plaquetas, o leucograma e os sinais clínicos, nenhuma diferença significativa (P> 0,05) foi observada entre cães soropositivos e soronegativos para E. canis ou para B. vogeli. Os resultados sorológicos deste estudo sugerem que a Cienc. anim. bras., Goiânia, v.18, 1-9, e-36095, 2017 2 FONSECA, J.P., et al.infecção por E. canis e B. vogeli é endêmica na população canina em questão, com uma prevalência da fase subclínica (assintomática) em cães soropositivos para erliquiose ou babesiose.

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“…Cardozo et al 2007 analisaram o gene 16S rRNA da A. Platys detectado pela nPCR e descreveram a importância do seqüenciamento do DNA para desenvolvimento da especiϐicidade e sensibilidade dos exames moleculares e para melhor caracterizar as linhagens circulantes no Brasil. A incidência de A. platys no Brasil é baixa (Macieira et al 2005) e a caracterização molecular envolvendo a comparação de seqüências de DNA de cepas brasileiras com cepas ao redor do mundo é muito importante, porém, neste estudo não foi possível a realização do seqüenciamento das amostras positivas.…”

Section: Methodsunclassified

Investigação molecular de Ehrlichia spp. e Anaplasma platys em felinos domésticos: alterações clínicas, hematológicas e bioquímicas

et al. 2011

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ABSTRACT.-Corrêa E.S., Paludo G.R., Scalon M.C., Machado J.A., Lima A.C.Q., Pinto A. Ehrlichia sp. and Anaplasma platys are Gram-negative micro-organisms, obligate intracellular parasites, residing in cytoplasmic vacuoles of leukocytes and platelets, found in peripheral blood or tissue. Few reports have been made about ehrlichiosis and anaplasmosis in cats in Brazil, which are based on the presence of morulae in leukocytes and platelets, or by detecting antibodies. The objective of this study was to investigate the natural infection with Ehrlichia sp. and A. platys in cats in Campos dos Goytacazes-RJ, by hematoscopia and DNA detection of these agents. Samples of whole blood and serum from 91 cats, regardless of race, gender and age. Blood count, serum biochemistry and PCR using primers for Ehrlichia sp. and A. platys were perfomed. Data from hematoscopia showed 9.89% of morulae only in platelets. The DNA of A. platys was detected in 13.18% of the 91 samples and 44.44% of the positive at hematoscopia. The DNA of Ehrlichia sp. was not detected in any sample. All animals studied did not show clinical signs neither positive laboratory results. The data suggest that domestic cats can serve as potential reservoirs for A. platys as asymptomatic form of related diseases.

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Prevalence ofEhrlichia canisinfection in thrombocytopenic dogs from Rio de Janeiro, Brazil

Cited by 54 publications

References 26 publications

Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

Investigação molecular de Ehrlichia spp. e Anaplasma platys em felinos domésticos: alterações clínicas, hematológicas e bioquímicas

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