Prevalence of
            <i>Escherichia coli</i>
            O157 and
            <i>Salmonella</i>
            spp. in solid bovine manure in California using real‐time quantitative PCR

Zhao, Chen; Biswas, Sagor; Aminabadi, Peiman; Stackhouse, J. W.; Jay-Russell, Michele; Pandey, Pramod Kumar

doi:10.1111/lam.13156

Cited by 10 publications

(11 citation statements)

References 31 publications

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“…Salmonella were detected in PS (2017), DLE (2018), and SL (2018) treatments and further transferred to the amended soil samples. In support of our finding, 6.6% of solid bovine manure collected from dairy farms in California was positive of Salmonella (Chen et al, 2019). Salmonella has been detected in more than 80% of effluent bovine manure immediately post-commercial anaerobic digestion (Chiapetta et al, 2019).…”

Section: Discussionsupporting

confidence: 81%

“…The Salmonella in raw manure (SL) was below our detection limit of 3 MPN/g while DLE was 55.5 ± 12.5 MPN/g, higher than the microbial standard of 3 MPN/4 g for treated manure (FDA, 2018b). A recent study reported that Salmonella in raw dairy manure ranged from less than 3 Log 10 CFU/g to 5.2 Log 10 CFU/g (Chen et al, 2019). Farm-scale psychrophilic anaerobic digester only reduced Salmonella by 1.2–1.4 Log 10 CFU/g in swine manure (initial Salmonella population ranged from less than 2.0 to 4.7 Log 10 CFU/g), resulting in fair amount of Salmonella remaining in the effluent manure (Masse et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Microbial Safety of Dairy Manure Fertilizer Application in Raspberry Production

et al. 2019

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Dairy manure, a by-product in the dairy industry, is also a potential source of nutrients for crops. However, improper application of biological soil amendments of animal origin can be a source of contamination with enteric foodborne pathogens. A 2-year field study was conducted to evaluate impacts of dairy manure fertilizer application on the microbial safety of red raspberry (Rubus idaeus L) production. Fertilizers, including a standard synthetic fertilizer (CON), straight lagoon raw manure (SL), anaerobically digested liquid effluent (DLE), compost (COM) and dairy manure-derived refined fertilizers including ammonium sulfate (AS) and phosphorous solid (PS), were randomly applied in quadruplicate to raspberry plots. Soil, fertilizer, foliar, and raspberry fruit samples were collected during the cropping season for the quantification of indicator microorganisms (total coliform and generic Escherichia coli) and detection of important foodborne pathogens (Shiga toxin-producing E. coli (STEC), Salmonella, and Listeria monocytogenes). Counts of total coliforms in soil were stable over the 2017 cropping season and were not impacted by fertilizer application. In 2018, total coliforms increased with season and soils treated with COM had a significantly higher coliform number than those treated with CON. Both total coliform and generic E. coli in raspberry fruit samples were below the detectable level (3 most probable number/g) regardless of fertilizer types. In both years, no STEC or L. monocytogenes was detected from any of the collected samples regardless of fertilizer treatments. However, Salmonella were detected in some of the fertilizers, including PS (2017), DLE (2018), and SL (2018), which were transferred to soil samples taken directly after application of these fertilizers. Salmonella were not detected in soil samples 2 or 4 months post fertilizer application, foliar, or raspberry fruit samples regardless of fertilizer applications. In summary, one-time application of raw dairy manure or dairy manure-derived fertilizers more than 4 months prior to harvest has no major impact on food safety of red raspberry (6 ft. tall) production in Lynden sandy loam under good agricultural practices.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Microbial Safety of Dairy Manure Fertilizer Application in Raspberry Production

et al. 2019

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“…Variation in management practices among the farms may reflect the spatial-temporal clustering of positive samples, highlighting the importance of surveying multiple farms and conducting multi-year studies. However, we found infrequent (low) E. coli O157:H7 prevalence (1.3%), in only poultry and mixed manure samples, whereas other studies reported a prevalence of 15.4% in solid bovine manure from a multi-county survey in California (Chen et al, 2019) and 19% in bovine manure (multiple storage type and ages) in the Southeastern region of the US (Baker et al, 2019). Cattle and other ruminants are commonly considered to be natural reservoirs for non-O157 STEC, with cattle being the major reservoir of E. coli O157:H7 (Hutchison et al, 2005;Jay et al, 2007;Berry et al, 2015Berry et al, , 2019.…”

Section: Pathogen Detection In Manurecontrasting

confidence: 89%

Survival and Persistence of Foodborne Pathogens in Manure-Amended Soils and Prevalence on Fresh Produce in Certified Organic Farms: A Multi-Regional Baseline Analysis

Ramos

Jay-Russell

Millner

et al. 2021

Front. Sustain. Food Syst.

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Biological soil amendments of animal origin (BSAAOs), including untreated (e.g., raw or aged manure, or incompletely composted manure) and treated animal products (e.g., compost), are used for crop production and as part of soil health management. Application of BSAAO's must be done cautiously, as raw manure commonly contains enteric foodborne pathogens that can potentially contaminate edible produce that may be consumed without cooking. USDA National Organic Program (NOP) certified production systems follow the 90-or 120-day interval standards between applications of untreated BSAAOs and crop harvest, depending on whether the edible portions of the crops are in indirect or direct contact with the soil, respectively. This study was conducted to evaluate the survival of four foodborne pathogens in soils amended with BSAAOs and to examine the potential for bacterial transfer to fresh produce harvested from USDA NOP certified organic farms (19) from four states. Only 0.4% (2/527) of produce samples were positive for L. monocytogenes. Among the untreated manure and compost samples, 18.0% (42/233) were positive for at least one of the tested and culturable bacterial foodborne pathogens. The prevalence of non-O157 STEC and Salmonella in untreated manure was substantially > that of E. coli O157:H7 and L. monocytogenes. Of the 2,461 soil samples analyzed in this study, 12.9% (318) were positive for at least one pathogen. In soil amended with untreated manure, the prevalence of non-O157 STEC [7.7% (190) and L. monocytogenes (5.0% (122), was > that of Salmonella (1.1% (26)] or E. coli O157 [0.04% (1)]. Foodborne pathogen prevalence in the soil peaked after manure application and decreased significantly 30 days post-application (dpa). However, non-O157 STEC and L. monocytogenes were recovered from soil samples after 90 and 120 dpa. Results indicate that produce contamination by tested foodborne pathogens was infrequent, but these data should not be generalized outside of the specific wait-time regulations for organic crop production and the farms studied. Moreover, other sources of contamination, e.g., irrigation, wildlife, environmental conditions, cropping and management practices, should be considered. This study also provides multi-regional baseline data relating to current NOP application intervals and development of potential risk mitigation strategies to reduce pathogen persistence in soils amended with BSAAOs. These findings contribute to filling critical data gaps concerning occurrence of fecal pathogens in NOP-certified farming systems used for production of fresh produce in different US regions.

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“…Organic fertilizer can accelerate mineralization of organic phosphorus by microorganisms by affecting the activity of soil extracellular phosphatase (Luo et al, 2019). In order to ensure public health and safety, Chen, Biswas, et al (2019) conducted quantitative PCR analysis on the levels of Escherichia coli and Salmonella in untreated cow dung. The level of these two bacteria in untreated cow dung is higher than that in underground samples.…”

Section: Application Of Molecular Biology Methods In Soil Improvementmentioning

confidence: 99%

Molecular biological methods in environmental engineering

Zhang

et al. 2020

Water Environment Research

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Microbes are sensitive to environmental changes and can respond in a short time. Genomics, proteomics, transcriptomics, metabolomics, and multigroup association are used to characterize the composition, function, and metabolism of microorganisms, and to evaluate the environment according to the changes in microorganisms, which has important reference and guiding significance of environmental monitoring, management, and repair. In this paper, the application of molecular biological methods to study environmental microorganisms in the fields of wastewater treatment, pollution control, soil improvement, and environmental monitoring in 2019 is reviewed.

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Prevalence of Escherichia coli O157 and Salmonella spp. in solid bovine manure in California using real‐time quantitative PCR

Cited by 10 publications

References 31 publications

Microbial Safety of Dairy Manure Fertilizer Application in Raspberry Production

Microbial Safety of Dairy Manure Fertilizer Application in Raspberry Production

Survival and Persistence of Foodborne Pathogens in Manure-Amended Soils and Prevalence on Fresh Produce in Certified Organic Farms: A Multi-Regional Baseline Analysis

Molecular biological methods in environmental engineering

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