Prevalence of<i>Gardnerella vaginalis</i>and<i>Atopobium vaginae</i>in Portuguese women and association with risk factors for bacterial vaginosis

Silva, Debora; Henriques, Ana Filipa Frutuoso Mendes; Cereija, T.B.; Martinez-de-Oliveira, José; Miranda, Manuela; Cerca, Nuno

doi:10.1016/j.ijgo.2013.08.008

Cited by 5 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A significant higher colonization by G. vaginalis not associated to BV has been reported elsewhere ( Cox et al, 2016 ; Janulaitiene et al, 2017 ). However, other epidemiological studies reported G. vaginalis colonization rates much lower than ours ( Pépin et al, 2011 ; Schwebke, Flynn & Rivers, 2014 ; Silva et al, 2014 ). These differences might be the result of significant different populations at study.…”

Section: Discussioncontrasting

confidence: 89%

“…However, the great diversity of vaginal microbiota in women with BV affects its potential to develop preterm birth and other negative outcomes in pregnancy ( Hyman et al, 2014 ; Nelson et al, 2015 ) and it becomes BV treatment in pregnant women a challenging task ( Haahr et al, 2016 ). Despite of its high impact, few epidemiological studies on BV have been conducted in Portugal ( Guerreiro, Gigante & Teles, 1998 ; Silva et al, 2014 ; Machado et al, 2015 ). Specifically, there is no information related to the prevalence of BV among Portuguese pregnant women.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of bacterial vaginosis in Portuguese pregnant women and vaginal colonization byGardnerella vaginalis

Machado

Castro

Martinez-de-Oliveira

et al. 2017

PeerJ

Self Cite

View full text Add to dashboard Cite

BackgroundWe aimed to determine the prevalence of vaginal colonization by Gardnerella vaginalis and of bacterial vaginosis (BV) in Portuguese pregnant women, and to identify risk factors for BV and G. vaginalis colonization in pregnancy.MethodsA cross-sectional study was conducted among pregnant women aged ≥ 18 years who were attending in two public hospitals of the Northwest region of Portugal. Epidemiological data was collected by anonymous questionnaire. BV was diagnosed by Nugent criteria and G. vaginalis presence was identified by polymerase chain reaction. Crude associations between the study variables and BV or G. vaginalis colonization were quantified by odds ratios (ORs) and their 95% confidence intervals (CIs).ResultsThe prevalences of BV and of G. vaginalis colonization among Portuguese pregnant women were 3.88% and 67.48%, respectively. Previous preterm delivery and colonization by G. vaginalis were factors with very high OR, but only statistically significant for a 90% CI. Conversely, higher rates of G. vaginalis colonization were found in women with basic educational level (OR = 2.77, 95% CI [1.33–5.78]), during the second trimester of pregnancy (OR = 6.12, 95% CI [1.80–20.85]) and with BV flora (OR = 8.73, 95% CI [0.50–153.60]).DiscussionDespite the lower number of women with BV, prevalence ratios and association with risk factors were similar to recent European studies. However, the percentage of healthy women colonized by G. vaginalis was significantly higher than many previous studies, confirming that G. vaginalis colonization does not always lead to BV development.

show abstract

Section: Discussioncontrasting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Prevalence of bacterial vaginosis in Portuguese pregnant women and vaginal colonization byGardnerella vaginalis

Machado

Castro

Martinez-de-Oliveira

et al. 2017

PeerJ

Self Cite

View full text Add to dashboard Cite

show abstract

“…Therefore, vaginal bacterial communities differ dramatically between healthy patients and patients with BV, where G. vaginalis is present in over 90% of BV cases ( Verstraelen & Swidsinski, 2013 ). The role of G. vaginalis is still controversial, since this bacterium is also present in 10–40% of healthy women ( Aroutcheva et al, 2001 ; Hickey & Forney, 2014 ; Silva et al, 2014 ); however, recent evidence suggests that the presence of G. vaginalis biofilms, instead of dispersed cells, are in fact an indication of BV ( Verstraelen & Swidsinski, 2013 ). Furthermore, we recently demonstrated that G. vaginalis has a significantly higher virulence potential than other 29 BV associated species, including a higher cytotoxicity and biofilm formation ability ( Alves et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of bacterial vaginosis by a new multiplex peptide nucleic acid fluorescencein situhybridization method

Machado

Castro

Cereija

et al. 2015

PeerJ

Self Cite

View full text Add to dashboard Cite

Bacterial vaginosis (BV) is one of most common vaginal infections. However, its diagnosis by classical methods reveals low specificity. Our goal was to evaluate the accuracy diagnosis of 150 vaginal samples with research gold standard methods and our Peptide Nucleic Acid (PNA) probes by Fluorescence in situ Hybridization (FISH) methodology. Also, we described the first PNA-FISH methodology for BV diagnosis, which provides results in approximately 3 h. The results showed a sensitivity of 84.6% (95% confidence interval (CI), from 64.3 to 95.0%) and a specificity of 97.6% (95% CI [92.6–99.4%]), demonstrating the higher specificity of the PNA-FISH method and showing false positive results in BV diagnosis commonly obtained by the classical methods. This methodology combines the specificity of PNA probes for Lactobacillus species and G. vaginalis visualization and the calculation of the microscopic field by Nugent score, allowing a trustful evaluation of the bacteria present in vaginal microflora and avoiding the occurrence of misleading diagnostics. Therefore, the PNA-FISH methodology represents a valuable alternative for BV diagnosis.

show abstract

“…Several of these taxa have species that are opportunistic pathogens. A. vaginae, the most popularly studied Atopobium species, has been implicated in increased pH (47) and pathogenesis in bacterial vaginosis (48,49). Although these bacteria were not identi ed to the species level in the current study or in others that have also found associations with SARS-CoV-2 infection, their general lactic acid-producing characteristics and the formation of bio lms (50) support their links with the naso-oropharynx in COVID-19 infections.…”

Section: Discussionmentioning

confidence: 47%

High abundance of butyrate-producing bacteria in the naso-oropharynx of SARS-CoV-2-infected persons in an African population: implications for low disease severity

Akorli,

Opoku,

Appiah-Twum

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Microbiome dysbiosis is associated with various diseases, including COVID-19. The association of the oral microbiome with SARS-CoV-2 infections and disease progression has been documented in European, Asian, and American populations but not in Africa. Methods: We conducted a study in Ghana to evaluate and compare the naso-oropharyngeal microbiome in SARS-CoV-2-infected and noninfected persons before and after vaccination. 16S rDNA was sequenced and analysed from DNA extracted from the naso-oropharyngeal swabs of consenting participants. Results: Alpha diversity was high among pre-vaccinated virus-positive individuals (Shannon: p< 0.0001) but reduced among vaccinated persons. Contrary to other reports, differences in viral loads did not significantly affect alpha diversity. Pre-vaccinated SARS-CoV-2-positive and -negative individuals had little yet significant microbial compositional dissimilarity (PERMANOVA: R2=0.14, p= 0.001) but not when some individuals were vaccinated (PERMANOVA: R2=0.013, p= 0.49). Consistent with other studies, Prevotella and Atopobium were abundant in pre-vaccinated virus-positive persons (adjusted p value <0.05). Butyrate-producing microbes, including members of Lachnospiraceae and Fusobacterium sp., were in relatively high abundances in infected individuals. As biomarkers associated with the infection (log10LDA> 4.0), they suggest probable protective pathophysiological processes that would prevent severe disease outcomes in this population. Anaerovoracaceae was increased in infected vaccinated persons, further implicating Firmicutes in protective immunity against COVID-19. Conclusion: Our results necessitate further studies to confirm the integral role of Firmicutes in immune responses and disease progression. We also recommend expansion of microbiome–disease association studies across Africa to identify possible bacterial-mediated therapeutics for emerging infections.

show abstract

Prevalence ofGardnerella vaginalisandAtopobium vaginaein Portuguese women and association with risk factors for bacterial vaginosis

Cited by 5 publications

References 5 publications

Prevalence of bacterial vaginosis in Portuguese pregnant women and vaginal colonization byGardnerella vaginalis

Prevalence of bacterial vaginosis in Portuguese pregnant women and vaginal colonization byGardnerella vaginalis

Diagnosis of bacterial vaginosis by a new multiplex peptide nucleic acid fluorescencein situhybridization method

High abundance of butyrate-producing bacteria in the naso-oropharynx of SARS-CoV-2-infected persons in an African population: implications for low disease severity

Contact Info

Product

Resources

About