Prevalence of <i>Staphylococcus aureus</i> and antibiotic resistant <i>Staphylococcus aureus</i> in public transport in Bratislava, Slovakia

Medveďová, Alžbeta; Györiová, Romana

doi:10.2478/acs-2019-0007

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Staphylococcus aureus is a ubiquitous, Gram-positive pathogen that causes a diverse range of bacterial infections, from skin and soft tissue infections to potentially fatal infections, such as pneumonia, endocarditis, osteomyelitis, sepsis, and toxic shock syndrome (1). Coupled with the growing prevalence of methicillin-resistant S. aureus (MRSA) strains, the worldwide threat posed by this pathogen is obvious (2). Current research provides insights into important features of these strains, including antibiotic resistance and extensive virulence factors.…”

Section: Introductionmentioning

confidence: 99%

Elucidating the CodY regulon inStaphylococcus aureusUSA300 substrains

Gao

Poudel

Seif

et al. 2021

Preprint

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CodY is a conserved broad acting transcription factor that regulates the expression of genes related to amino acid metabolism and virulence in methicillin-resistant Staphylococcus aureus (MRSA). CodY target genes have been studied by using in vitro DNA affinity purification and deep sequencing (IDAP-Seq). Here we performed the first in vivo determination of CodY target genes using a novel CodY monoclonal antibody in established ChIP-exo protocols. Our results showed, 1) the same 135 CodY promoter binding sites regulating 165 target genes identified in two closely related virulent S. aureus USA300 TCH1516 and LAC strains; 2) The differential binding intensity for the same target genes under the same conditions was due to sequence differences in the same CodY binding site in the two strains; 3) Based on transcriptomic data, a CodY regulon comprising 72 target genes that are differentially regulated relative to a CodY deletion strain, representing genes that are mainly involved in amino acid transport and metabolism, inorganic ion transport and metabolism, transcription and translation, and virulence; and 4) CodY systematically regulated central metabolic flux to generate branched-chain amino acids (BCAAs) by mapping the CodY regulon onto a genome-scale metabolic model of S. aureus. Our study performed the first system-level analysis of CodY in two closely related USA300 TCH1516 and LAC strains giving new insights into the similarities and differences of CodY regulatory roles between the closely related strains.ImportanceWith the increasing availability of whole genome sequences for many strains within the same pathogenic species, a comparative analysis of key regulators is needed to understand how the different strains uniquely coordinate metabolism and expression of virulence. To successfully infect the human host, Staphylococcus aureus USA300 relies on the transcription factor CodY to reorganize metabolism and express virulence factors. While CodY is a known key transcription factor, its target genes are not characterized on a genome-wide basis. We performed a comparative analysis to describe the transcriptional regulation of CodY between two dominant USA300 strains. This study motivates the characterization of common pathogenic strains and an evaluation of the possibility of developing specialized treatments for major strains circulating in the population.

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Section: Introductionmentioning

confidence: 99%

Elucidating the CodY regulon inStaphylococcus aureusUSA300 substrains

Gao

Poudel

Seif

et al. 2021

Preprint

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Factors affecting the exposure to physicochemical and microbiological pollutants in vehicle cabins while commuting in Lisbon

Buitrago

Savdie

Almeida

et al. 2021

Environmental Pollution

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An overview of the bacterial microbiome of public transportation systems—risks, detection, and countermeasures

Ly,

Leuko,

Moeller

2024

Front. Public Health

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When we humans travel, our microorganisms come along. These can be harmless but also pathogenic, and are spread by touching surfaces or breathing aerosols in the passenger cabins. As the pandemic with SARS-CoV-2 has shown, those environments display a risk for infection transmission. For a risk reduction, countermeasures such as wearing face masks and distancing were applied in many places, yet had a significant social impact. Nevertheless, the next pandemic will come and additional countermeasures that contribute to the risk reduction are needed to keep commuters safe and reduce the spread of microorganisms and pathogens, but also have as little impact as possible on the daily lives of commuters. This review describes the bacterial microbiome of subways around the world, which is mainly characterized by human-associated genera. We emphasize on healthcare-associated ESKAPE pathogens within public transport, introduce state-of-the art methods to detect common microbes and potential pathogens such as LAMP and next-generation sequencing. Further, we describe and discuss possible countermeasures that could be deployed in public transportation systems, as antimicrobial surfaces or air sterilization using plasma. Commuting in public transport can harbor risks of infection. Improving the safety of travelers can be achieved by effective detection methods, microbial reduction systems, but importantly by hand hygiene and common-sense hygiene guidelines.

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Prevalence of Staphylococcus aureus and antibiotic resistant Staphylococcus aureus in public transport in Bratislava, Slovakia

Cited by 3 publications

References 11 publications

Elucidating the CodY regulon inStaphylococcus aureusUSA300 substrains

Elucidating the CodY regulon inStaphylococcus aureusUSA300 substrains

Factors affecting the exposure to physicochemical and microbiological pollutants in vehicle cabins while commuting in Lisbon

An overview of the bacterial microbiome of public transportation systems—risks, detection, and countermeasures

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