Prevalence of Xanthomonas euvesicatoria (formally X. perforans) associated with bacterial spot severity in Capsicum annuum crops in South Central Chihuahua, Mexico

Hernández-Huerta, Jared; Tamez‐Guerra, Patricia; Gómez-Flores, Ricardo; Delgado-Gardea, Ma Carmen E; García-Madrid, Margarita S; Robles-Hernández, Loreto; Infante-Ramírez, Rocío

doi:10.7717/peerj.10913

Cited by 13 publications

(5 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…X. perforans, formerly known as bacterial spot pathogen on tomato only, has recently been shown to have expanded its host range to pepper as an emerging pathogen [2,3,9,10]. We found X. perforans to be highly pathogenic on pepper and weakly pathogenic on eggplant, supportive of the host expansion.…”

Section: Resultssupporting

confidence: 57%

Section: Resultsmentioning

confidence: 67%

“…However, in the last decade, a host range shift in the X. perforans population to include pepper as a host has been observed in North America [2, 3, 9, 10]. Surveys of Xanthomonas populations in other growing regions are needed to identify host-range shifts.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Draft genome sequences of 11 Xanthomonas strains associated with bacterial spot disease in Turkey

Subedi

Kara²,

Aysan

et al. 2023

Access Microbiology

View full text Add to dashboard Cite

Bacterial spot is an economically significant disease in tomato and pepper-producing countries globally. We report the whole-genome sequence of 11 Xanthomonas strains associated with bacterial spot disease on pepper, tomato and eggplant in the Southeastern Anatolia Region, Turkey. This genomic information can be used as a reference to study the genetic diversity of these species and contribute to illuminating pathogen evolution with respect to host specificity.

show abstract

Section: Resultssupporting

confidence: 57%

Section: Resultsmentioning

confidence: 67%

See 1 more Smart Citation

Draft genome sequences of 11 Xanthomonas strains associated with bacterial spot disease in Turkey

Subedi

Kara²,

Aysan

et al. 2023

Access Microbiology

View full text Add to dashboard Cite

show abstract

“…9). According to Hernández-Huerta et al (2021), Xanthomonads were grouped into three groups in a cluster analysis of bacterial spot severity according to their virulence and findings that isolated X. euvesicatoria caused medium damage 76.8% final severity and low damage 29.8% final severity.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomic Analysis and Rapid Molecular Detection of Xanthomonas euvesicatoria Using Unique ATP-Dependent DNA Helicase recQ, hrpB1, and hrpB2 Genes Isolated from Physalis pubescens in China

Siddique

Yang

et al. 2023

Plant Pathol J

View full text Add to dashboard Cite

Ground cherry (Physalis pubescens) is the most prominent species in the Solanaceae family due to its nutritional content, and prospective health advantages. It is grown all over the world, but notably in northern China. In 2019 firstly bacterial leaf spot (BLS) disease was identified on P. pubescens in China that caused by both BLS pathogens Xanthomonas euvesicatoria pv. euvesicatoria resulted in substantial monetary losses. Here, we compared whole genome sequences of X. euvesicatoria to other Xanthomonas species that caused BLS diseases for high similarities and dissimilarities in genomic sequences through average nucleotide identity (ANI) and BLAST comparison. Molecular techniques and phylogenetic trees were adopted to detect X. euvesicatoria on P. pubescens using recQ, hrpB1, and hrpB2 genes for efficient and precise identification. For rapid molecular detection of X. euvesicatoria, loop-mediated isothermal amplification, polymerase chain reaction (PCR), and real-time PCR techniques were used. Whole genome comparison results showed that the genome of X. euvesicatoria was more closely relative to X. perforans than X. vesicatoria, and X. gardneri with 98%, 84%, and 86% ANI, respectively. All infected leaves of P. pubescens found positive amplification, and negative controls did not show amplification. The findings of evolutionary history revealed that isolated strains XeC10RQ, XeH9RQ, XeA10RQ, and XeB10RQ that originated from China were closely relative and highly homologous to the X. euvesicatoria. This research provides information to researchers on genomic variation in BLS pathogens, and further molecular evolution and identification of X. euvesicatoria using the unique target recQ gene through advance molecular approaches.

show abstract

“…The amplification was carried out by using the universal primers 27f and 1525r [15]. Subsequently, these strains were also reconfirmed using the specific primer Bs-Xpf/Bs-Xpr of X. perforans [16]. All the bacterial strains, including B. amyloliquefaciens PMB04 [11] and X. perforans, were cultured on nutrient agar (NA) plates at 28 • C for 48 h.…”

Section: Growth Conditions For Plants and Bacteriamentioning

confidence: 99%

Developing Fermentation Liquid of Bacillus amyloliquefaciens PMB04 to Control Bacterial Leaf Spot of Sweet Pepper

et al. 2023

View full text Add to dashboard Cite

Sweet pepper is an important vegetable in the world. Bacterial leaf spot, caused by the pathogen Xanthomonas perforans, is a limiting factor that significantly reduces the quality and yield of sweet peppers. The use of chemical fungicides is currently the main disease-control method for bacterial leaf spot disease. It is important to develop an eco-friendly biocontrol method by using antagonistic microorganisms. Bacillus amyloliquefaciens PMB04 has strong antagonistic effects against pathogens and can inhibit the occurrence of diseases. B. amyloliquefaciens PMB04 has the potential for the development of a disease-control product. Primarily, PMB04 contained a strong inhibitory effect against all isolated X. perforans strains. In the inoculation assay, the severity of bacterial leaf spot disease on sweet peppers was reduced by PMB04 bacterial suspensions. To increase the convenience of field applications in future prospects, the development of the PMB04 fermentation liquid was carried out using different ratios of brown sugar and yeast extract in a 30 L fermentation tank. The results exhibited that the fermentation liquid of the 3-1 and 2-1 formulas obtained the highest bacterial population in a 30 L fermentation tank. The fermentation liquid of the 0.5-0.5 formula was the most stable formula for two different conditions in terms of a consistent bacterial population and sporulation. In addition, the 200-fold dilution of the 3-1 and 0.5-0.5 fermentation liquids revealed the best control efficacy on bacterial leaf spot disease of sweet peppers. Additionally, the results of the 0.5-0.5 fermentation liquid (PMB4FL) with different dilution concentrations also demonstrated that the 200- and 500-fold dilutions had the best control efficacy. To understand the effect of commonly used copper-containing fungicides on sweet peppers on the application of microbial agent PMB4FL, the effects of copper hydroxide and tribasic copper sulfate on the growth of X. perforans strains and B. amyloliquefaciens PMB04 were assayed. The results exhibited that the above two fungicides did not have any inhibitory effect on the growth of PMB04 but had a strong inhibitory effect on the X. perforans strain. In the follow-up control experiment, the treatment of copper hydroxide had no synergistic effect with PMB4FL to control bacterial leaf spot disease. We concluded that the use of the PMB4FL fermentation liquid alone on the leaves could effectively control the occurrence of bacterial leaf spots in sweet pepper crops.

show abstract

Prevalence of Xanthomonas euvesicatoria (formally X. perforans) associated with bacterial spot severity in Capsicum annuum crops in South Central Chihuahua, Mexico

Cited by 13 publications

References 40 publications

Draft genome sequences of 11 Xanthomonas strains associated with bacterial spot disease in Turkey

Draft genome sequences of 11 Xanthomonas strains associated with bacterial spot disease in Turkey

Comparative Genomic Analysis and Rapid Molecular Detection of Xanthomonas euvesicatoria Using Unique ATP-Dependent DNA Helicase recQ, hrpB1, and hrpB2 Genes Isolated from Physalis pubescens in China

Developing Fermentation Liquid of Bacillus amyloliquefaciens PMB04 to Control Bacterial Leaf Spot of Sweet Pepper

Contact Info

Product

Resources

About