Prevalence of impaired responsiveness to epinephrine in platelets among Japanese

Kambayashi, Jun-ichi; Shinoki, Nobutoshi; Nakamura, Takashi; Ariyoshi, Hideo; Kawasaki, Tomio; Sakon, Masato; Monden, Morito

doi:10.1016/0049-3848(95)00216-2

Cited by 46 publications

(38 citation statements)

References 12 publications

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“…These results are very similar to the study on healthy donors described above, which reported 16% of healthy individuals as non-responders to adrenaline [33]. Our study, showing interindividual variation in the response to adrenaline combined with LPA, further supports the claim that the response to adrenaline in the ET population is highly heterogeneous.…”

Section: Discussionsupporting

confidence: 81%

“…Further, we observed a considerable individual variability both in controls and in ET patients regarding the combination of adrenaline and LPA. The appearance of individual variability in the response to adrenaline has been studied in the healthy population, and when including 140 individuals, 16% were classified as non-responders to adrenaline-induced platelet aggregation in plasma [33]. This variability in the adrenaline response in healthy subjects has been confirmed by other investigations also studying platelet function in plasma [20,[34][35][36].…”

Section: Discussionsupporting

confidence: 52%

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Enhanced platelet adhesion in essential thrombocythemia after in vitro activation

Eriksson¹,

Lotfi²,

Whiss³

2010

Turk J Hematol

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Objective: Essential thrombocythemia (ET) is a chronic myeloproliferative disorder characterized by elevated platelet counts and increased risk of thrombosis. Ex vivo data suggest increased platelet reactivity in agreement with the increased thrombosis risk, while in vitro tests often detect decreased platelet activity. The present study aimed to investigate adhesion of ET-platelets in vitro, which is an aspect of platelet function that has been addressed in only a few studies on ET patients. Material and Methods: The study included 30 ET patients and 14 healthy controls. Platelet adhesion was measured with a static platelet adhesion assay. Results: The main finding was that ET-platelets were more readily activated by adhesion-inducing stimuli in vitro than control platelets. This was particularly evident in elderly patients and when using multiple stimuli, such as surfaces of collagen or fibrinogen combined with addition of adenosine 5'-diphosphate or ristocetin. Such multiple stimuli resulted in adhesion above the control mean +2 standard deviations for approximately 50% of the patients. Conclusion:The results are in accordance with the concept of increased platelet activity in ET, but opposite to most other in vitro studies. We suggest that the conditions in the adhesion assay might mimic the in vivo situation regarding the presence of chronic platelet activation. ÖzetAmaç: Esansiyel trombositemi (ET) platelet sayısının artması ve yüksek tromboz riski ile karakterize kronik bir myeloproliferatif bozukluktur. Ex vivo veriler tromboz riskine uygun olarak artan platelet reaktivitesini öne sürerken in vitro testler sıklıkla platelet aktivitesinde azalma tespit etmektedir. Bu çalışmanın amacı ET-hastalarında az sayıda çalışmaya dahil edilmiş bir platelet fonksiyonu konusu olan ET-plateletleri adezyonunun in vitro incelenmesidir. Yöntem ve Gereçler: Çalışmaya 30 ET hastası ile 14 sağlıklı kontrol dahil edilmiştir. Statik platelet adezyonu tayini ile platelet adezyonu ölçülmüştür. Bulgular: Temel bulgu ET plateletlerinin, in vitro adezyon indüklenmiş uyaranlar ile kontrol plateletlerinden daha kolay aktive olduğu olmuştur. Bu durum özellikle yaşlı hastalarda ve adenosin 5-difosfat ya da ristosetin eklenerek kombine edilmiş kolajen ya da fibrinojen yüzeyler gibi çoklu uyaran kullanıldığında barizdir. Bu gibi çoklu uyaran hastaların yaklaşık %50'sinde kontrol değeri + 2 standart sapmanın üzerinde adezyon sonucunu vermiştir.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 52%

Enhanced platelet adhesion in essential thrombocythemia after in vitro activation

Eriksson¹,

Lotfi²,

Whiss³

2010

Turk J Hematol

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“…Impaired responsiveness of platelets to epinephrine and other catecholamines (CA) has been reported in approximately 20% of samples from healthy normal Korean and Japanese individuals (6,7). The degrees of aggregation in response to other aggregation-inducing agents (AA, ADP, and U46619) are also significantly lower in platelets that are hyporesponsive to CA (8,9).…”

Section: Introductionmentioning

confidence: 99%

PKC inhibitors RO 31-8220 and Gö 6983 enhance epinephrine-induced platelet aggregation in catecholamine hypo-responsive platelets by enhancing Akt phosphorylation

Kim

Kim²,

Kim³

et al. 2011

BMB Reports

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“…In studies of platelet function, they have absent aggregation with 6-10 mM epinephrine, and normal to reduced aggregation with ADP and collagen (Hayward et al, 1996(Hayward et al, , 1997. We felt there were limitations to using epinephrine aggregation for screening, as abnormalities can be seen in healthy individuals and in other disorders (Hayward et al, 1996(Hayward et al, , 1997Kambayashi et al, 1996;Rao et al, 1988;Weis & Lages, 1988). Platelet multimerin immunoassays and immunoblot testing for platelet glycoprotein proteolysis can be used to identify these patients (Hayward et al, 1996(Hayward et al, , 1997, but these assays are technically difficult, time-consuming, and are limited to specialized laboratories.…”

mentioning

confidence: 99%

Fibrinogen degradation products in patients with the Quebec platelet disorder

et al. 1997

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Summary. The Quebec platelet disorder is a serious bleeding disorder associated with proteolysis of a-granular proteins, including fibrinogen. We evaluated fibrinogen degradation product (FDP) assays as screening tests for this disorder. Patients with the Quebec platelet disorder (13/13) had elevated serum FDPs (P < 0 . 01) due to secreted degraded platelet fibrinogen, but normal plasma FDPs and D-dimers. Unrelated controls with bleeding disorders (32/34) had undetectable FDPs, and controls with FDPs due to disseminated intravascular coagulation (DIC) and other illnesses (11/11) had elevated FDPs in all assays (P < 0 . 01). Immunoblot analyses indicated plasma fibrinogen was normal in the Quebec patients and platelet FDPs were found in their platelet lysates, releasates and serum samples. Their platelet FDPs were not altered by treatment with fibrinolytic inhibitors and were different from the FDPs in DIC and in plasmin-digested fibrinogen. Tests for serum FDPs may provide a simple rapid way to screen for the Quebec platelet disorder.

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Prevalence of impaired responsiveness to epinephrine in platelets among Japanese

Cited by 46 publications

References 12 publications

Enhanced platelet adhesion in essential thrombocythemia after in vitro activation

Enhanced platelet adhesion in essential thrombocythemia after in vitro activation

PKC inhibitors RO 31-8220 and Gö 6983 enhance epinephrine-induced platelet aggregation in catecholamine hypo-responsive platelets by enhancing Akt phosphorylation

Fibrinogen degradation products in patients with the Quebec platelet disorder

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