Prevalence of infection and molecular confirmation by using ITS-2 region of Fasciola gigantica found in domestic cattle from Chiang Mai province, Thailand

Phalee, Anawat; Wongsawad, Chalobol

doi:10.1016/s1995-7645(14)60022-5

Cited by 12 publications

(10 citation statements)

References 23 publications

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“…Our results suggest that ITS-1 and ITS-2 sequences might be more useful for distinguishing between closely related taxa belonging to the family Clinostomidae than 28S sequences. This has also been suggested in previous studies (Curran et al 2006;Lotfy et al 2010;Phalee and Wongsawad 2014). The monophyletic origin of Euclinostomum has also been indicated in previous findings (Senapin et al 2014;Caffara et al 2016), as illustrated in ITS2 in the present study but not with other markers.…”

Section: Discussionsupporting

confidence: 92%

Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India

et al. 2022

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Platyhelminths belonging to the family Clinostomidae (Digenea) have a worldwide distribution and are known to infect piscivorous birds through their intermediate hosts, usually fish species. In the present study, clinostome metacercariae were collected from fish hosts, including Channa punctata (Bloch 1793) (n = 25) and Trichogaster fasciata Bloch and Schneider 1801 (n = 25), from a freshwater system in India. The experimental infection of cattle egrets, Ardea (Bubulcus) ibis Linnaeus 1758, with some of the live metacercariae found in the present study was successful. Live adult parasites were obtained from the buccal cavity of the birds. Both metacercaria and adult specimens were subjected to molecular studies to obtain the sequences of 28S, ITS1, and ITS2 (nuclear rDNA) regions. The parasites were found to belong to three species, Clinostomum giganticum Agarwal 1959; C. piscidium Southwell and Prashad 1918; and Euclinostomum heterostomum (Rudolphi 1809). Phylogenetic analyses of the sequences obtained from the adults and metacercariae established a link between the metacercariae in the fish hosts and adults in the avian host, which is essential to elucidate their partial life cycle and specify morphological characteristics in the metacercarial stage.

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Section: Discussionsupporting

confidence: 92%

Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India

et al. 2022

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“…In Thailand, the prevalence rates of fasciolosis in cattle and water buffaloes are estimated to be 52·94–67·27%, with the highest incidences in the North and North-east, and the lowest in the South. It has been reported that the Thailand annual economic loss due to fasciolosis in cattle and buffaloes is estimated around 350–400 million baht (US$10–11 million) owing to a lower nutrition conversion, a decrease in milk production and a reduction in fertility of the animals (Srihakim and Pholpark, 1991; Sukhapesna et al 1994; Anuracpreeda et al 2009 a , 2011, 2017 a ; Phalee and Wongsawad, 2014).…”

Section: Introductionmentioning

confidence: 99%

The in vitro anthelmintic activity of the ethanol leaf extracts of Terminalia catappa L. on Fasciola gigantica

et al. 2017

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At present, there are no medicinal plant extracts currently available for treatment and control of fasciolosis. The present work could provide, for the first study, conclusions on the in vitro fasciolicidal properties of the ethanol extract of Terminalia catappa L. (TcCE) leaves against adult Fasciola gigantica after incubation with RPMI-1640 medium containing the TcCE at various concentrations and times when compared with triclabendazole (TCZ). The relative motility and survival index values of the TcCE-treated flukes decreased at a more rapid rate than the TCZ-treated flukes. The death of the parasites was observed after exposed to TcCE at 3 h incubation with 400, 800 and 1000 µg mL-1, and at 6 h incubation in 100 and 200 µg mL-1. Vacuolization, blebbings and partial disruption on the parasites' tegument were observed by light microscopy. When examined by scanning electron microscopy, TcCE caused similar tegumental alterations in the parasites as those observed in TCZ treatment but with larger damage at comparative incubation periods, consisting of swelling, blebbing, disrupted blebs, loss of spines, leading to the erosion, lesion and eventual disruption of the total tegument. Therefore, the TcCE may exert its fasciolicidal effect against F. gigantica by initially causing the tegumental alteration.

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“…Miura et al (2005) reported the absence of heterozygosity in ITS-1 genes, which did not provide any support of gene fl ow among different lineages. Some workers suggested the ITS-2 as effective marker for species identifi cation, phylogenetic relations and developmental stages as well as molecular epidemiology and infection control (Rinaldi et al, 2005;Curran et al, 2006;Bazsalovicsová et al, 2010;Lotfy et al, 2010;Shylla et al, 2011;Ghatani et al, 2012;Phalee et al, 2014;Bian et al, 2013;Portier et al, 2012). In helminth parasites, ITS pseudogenes and mitochondrial genes are present and identifi cation of ITS region is not easy due to non-protein coding nature as compared to mitochondrial genes (Benasson et al, 2001).…”

Section: Resultsmentioning

confidence: 99%

A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region

et al. 2015

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SummaryThe rDNA region of eukaryotes has the immense potential to resolve the evolutionary and phylogeny problems using molecular markers. As evident from the present review, ITS region data is considered for interpretation of inter and intra-specifi c variations of 136 studies of 33 families including 78 genus and 114 species affecting individuals worldwide. Along with ITS-1 and ITS-2 region in 29 studies 18S region, in 38 studies 28S region and in 43 studies mitochondrial genes (COI and NDI) were also analyzed. Three new genera (Allobilharzia gen. nov., Caulanus gen. nov., and Latuterus gen. nov.) and 49 new species were discovered. Only 47 studies expressed variations at intra-specifi c and inter-specifi c level in complete ITS region, ITS-1 and ITS-2 rDNA sequences due to differences in nucleotide positions. According to the fi ndings ITS region is more reliable and precise marker for demarcation and identifi cation of species in combination of other DNA markers. Major studies were involved around the parasites of families Fasciolidae, Schistosomatidae, Opisthorchidae, Paragonimidae and Paramphistomidae, Clinostomidae, Diplostomidae, Haploporidae, among others infecting humans, farm animals, birds, fi shes, reptiles and amphibians on the clinical basis. In future, molecular and bioinformatics aspects based on genetic variations will lead to explore the untouched areas of trematodes.

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Prevalence of infection and molecular confirmation by using ITS-2 region of Fasciola gigantica found in domestic cattle from Chiang Mai province, Thailand

Cited by 12 publications

References 23 publications

Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India

Characterization of Clinostomum (Digenea: Clinostomidae) spp. in India

The in vitro anthelmintic activity of the ethanol leaf extracts of Terminalia catappa L. on Fasciola gigantica

A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region

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