Since anemia is one of the most skyrocketed public health problems worldwide, an investigation has thus been conducted to assess the prevalence and associated factors of anemia among the pregnant women receiving antenatal care (ANC) at Fatima Hospital in Jashore, Bangladesh. Facility-based crosssectional study was carried out among 384 pregnant women at Fatima hospital, Bangladesh from February to April, 2019. Data were obtained using a structured questionnaire and participant's current medical record card. Binary logistic regression analysis was used to identify factors associated with anemia and a P-value < 0.05 was considered as statistically significance. The overall prevalence of anemia among pregnant women was 58.9% (226/384). Out of 226 anemic pregnant mothers, 36.3% (82/226) were mildly anemic, 62.4% (141/226) were moderately anemic and 1.3% (3/384) was severely anemic. Binary logistic regression analysis revealed that the following variables were significantly associated with anemia: Monthly family income (11,000 -20,000 Taka) [AOR (95% CI) = 0.45 (0.21 -0.98)], Family size (joint) [AOR (95% CI) = 1.59 (1.03 -2.45)], Gestational age (third trimester) [AOR (95% CI) = 2.18 (1.40 -3.40)], birth spacing < 2 years [AOR (95% CI) = 2.87 (1.51 -5.44)], Excessive blood loss during previous surgery (Yes) [AOR (95% CI) = 2.08 (1.14 -4.17)], Food group eaten 24 hours (1 -4 groups) [AOR (95% CI) = 3.43 (1.84 -6.39)], Breakfast regularly (No) [AOR (95% CI) = 3.64 (1.36 -9.75)]. The results obtained showed that the prevalence of anemia among pregnant women is severe at Fatima Hospital in Jashore, Bangladesh.