Background: The present study was aimed to establish the prevalence, epidemiology and molecular characterization of major haemoprotozoons (Babesia and Theileria) and rickettsia (Anaplasma) of cattle in Jammu region (North India) using microscopy and Polymerase chain reaction (PCR). Hematology, Microscopy and PCR based prevalence studies were undertaken with 278 blood samples from cattle. Molecular prevalence studies were followed by genetic characterization of the isolates of Babesia, Anaplasma and Theileria spp. based on 18S rRNA, 16S rRNA and Tams1 gene, respectively. The data related to metrology and epidemiological variables like temperature, rainfall, season, age and type of livestock rearing was analyzed and correlated with disease by statistical methods. Results: The study revealed prevalence of Babesia spp., Anaplasma spp. and Theileria spp. to be 14.02%, 23.74% and 1.079% respectively. The metrological and epidemiological variables made inroads for the propagation of vector ticks and occurrence of infection. Haematological alterations predominantly related to haemoglobin, red blood cell count and packed cell volume were evident in diseased animals and collaterally affected the productivity. Further the genetic characterization of Babesia spp. (MN566925.1, MN567603, MN566924.1), Anaplasma spp. (MH733242.1, MN567602.1) and Theileria spp. (MT113479) provided a representative data of the isolates circulating in the region and their proximity with available sequences across the world.Conclusions: Despite holding much significance to the animal sector, comprehensive disease mapping has yet not been undertaken in several parts of India. The present study provides a blue print of disease mapping, epidemiological correlations and genomic diversity of Babesia spp., Anaplasma spp. and Theileria spp circulating in the region.