Prevalence of Leptospira spp. in Urban Rodents from a Groceries Trade Center of Medellín, Colombia

Agudelo‐Flórez, Piedad; Londoño, Andrés F.; Quiroz, Víctor H.; Ángel, Juan Edilberto Rendón; Moreno, Natali; Loaiza, Erica T; Muñoz, Luis F.; Rodas, Juan David

doi:10.4269/ajtmh.2009.09-0195

Cited by 55 publications

(48 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The pathogenesis of naturally-occurring leptospiral infection in rats has not been extensively studied and is complicated by a wide array of potentially confusing environmental factors that may also induce pathology in wild urban rats (11). The present study evaluated renal pathology in urban rats from a city where the prevalence of leptospiral isolation by kidney culture was approximately 20% (7). The finding of interstitial nephritis, however, cannot be attributed solely to leptospires since these rodents could also be exposed to other unknown environmental infectious agents that could cause interstitial nephritis (12).…”

Section: Discussionmentioning

confidence: 99%

“…Fifty milliliters of each macerated tissue sample was cultured in Fletcher semisolid medium followed by incubation at 28 to 30 °C. The cultures were examined under a dark-field microscope once per week during 4 months for positive or negative status (7). The other half of the second kidney was preserved at -70 °C for PCR Leptospira studies.…”

Section: Kidney Culture For Detection Of Leptospiramentioning

confidence: 99%

“…Trapping was conducted throughout 28 sessions during 7 months between August 2006 and April 2007. Rodents' capture, anesthesia, euthanasia, processing of tissue samples and follow up tests are previously described (7).…”

Section: Study Site Descriptionmentioning

confidence: 99%

See 2 more Smart Citations

Alteraciones histopatológicas en ratas naturalmente infectadas con Leptospira

et al. 2012

Self Cite

View full text Add to dashboard Cite

Introduction:Histopathological changes by Leptospira in naturally infected rodent reservoirs have been poorly described. Objective: The aim of the current study is to describe renal histopathology associated with leptospirosis infection of naturally infected rodents captured in the urban area of the city of Medellin, Colombia. Materials and methods: We performed hematoxilin-eosin (H-E) on kidney samples collected from 254 captured rodents. The positive samples were processed by Warthin Starry (W-S) staining and PCRLipL32. Results: Fifty one rodent kidneys showed H-E histopathological changes that consisted of inflammatory infiltrate with lympho-plasmocitary cells and histiocytes. We performed W-S staining and PCR-LipL32 to 67 kidney samples, including the 51 that had shown detectable changes by H-E and 16 (8%) of 203 rodents with negative results. Eight of the samples that tested positive for H-E (15.7%) were also positive for W-S staining. All negative for H-E were also negative for W-S staining. Of the W-S positive samples also tested for culture only three tested positive for both. Additionally, 47 (92.1%) samples positive for H-E were positive for PCR; while eleven of the 16 (68.8%) negative for H-E were positive for PCR. The samples positive for PCR were subsequently tested for culture and 11 (23.4%) were positive. Seven samples were positive for PCR and W-S and three were positive for PCR, W-S and culture. All of the PCR-LipL32 fragments were sequenced and showed specific amplicons for L. interrogans. Conclusions:The Leptospira infection was confirmed in all of the animals tested. The only histological kidney lesion attributable to leptospiral infection in the reservoir was interstitial nephritis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Kidney Culture For Detection Of Leptospiramentioning

confidence: 99%

See 1 more Smart Citation

Alteraciones histopatológicas en ratas naturalmente infectadas con Leptospira

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…Factors associated with the prevalence of the disease, as established through research, include: occupational factors, hygiene conditions, frequent contact with rodents, and owning/working with animals, all of which considerably increase the risks of contagion [22][23][24][25][26]. Medical treatment depends on the severity of the symptoms.…”

Section: Discussionmentioning

confidence: 99%

A retrospective analysis of the leptospirosis research in Colombia

Dechner

2014

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Leptospirosis is a re-emerging infectious disease whose prevalence is often underestimated, not only in Colombia, but in most developing countries. The objective of this paper is to assess the research status of leptospirosis in Colombia in order to identify trends, knowledge gaps, and directions for future research. Methodology: With the aim of gathering all the information available on leptospirosis in the country, a web search was conducted in various indexes and databases. The search terms used were: Leptospirosis + Colombia. A total of 95 publications were found and analyzed using descriptive statistics. Results: The first report of leptospirosis in the country was published in 1957. More than 60% of the published works focus on the prevalence of the disease. The Central part of the country is the area with the highest number of publications. MAT was used in 84% of the prevalence studies and humans are the most studied taxonomic group, with 45% of the publications. The highest prevalence of the disease was registered for rodents (82.7%, 62/75), followed by humans (67.9%, 53/78), pigs (67.6%, 342/506), and dogs (67.2%, 41/61). The prevalence of the disease in the country is associated with occupational factors, hygiene conditions and contact with rodents. Conclusion: Although leptospirosis is a prevalent disease in Colombia, the limitations of the diagnostic techniques used and the lack of a unified criteria on titers thresholds, make an accurate assessment of the prevalence of the disease in the country problematic.

show abstract

“…Las trampas fueron armadas y ubicadas durante la noche y se revisaron muy temprano en la mañana siguiente, antes de que el mercado se abriera al público. El muestreo descrito fue empleado originalmente para un estudio de leptospirosis en roedores sinantró-picos de la plaza minorista de Medellín, cuyos resultados fueron publicados previamente (13).…”

Section: Protocolo De Capturaunclassified

Rattus norvegicus como indicador de la circulación de Capillaria hepatica y Taenia taeniaeformis en la Plaza Minorista de Medellín, Colombia

et al. 2012

Self Cite

View full text Add to dashboard Cite

participaron en la recolección de la información, tanto de roedores como registros de histopatología. Diego Aranzazu hizo el análisis de los resultados histopatológicos y escritura del artículo. Juan D. Rodas y Piedad Agudelo-Flórez fueron los investigadores principales del proyecto inicial, realizaron el análisis de resultados, y la edición y corrección de estilo del artículo. ARTÍCULO ORIGINAL Biomédica 2012;32:510-8 doi: http://dx.doi.org/10.7705/biomedica.v32i4.442 Rattus norvegicus como indicador de la circulación de Capillaria hepatica y Taenia taeniaeformis en la Plaza Minorista de Medellín, Colombia Rattus norvegicus as an indicator of circulation of Capillaria hepatica and Taenia taeniaeformis on a groceries trade center of Medellín, ColombiaIntroduction. Rattus norvegicus, the Norway rat, plays a pivotal role in the maintenance and spread of several zoonotic bacterial, viral and parasitic pathogens of public health interest. The presence of helminthic infections near susceptible human populations can, under appropriate environmental conditions, become a risk factor for their transmission.Objective. Frequencies of infection were reported for Capillaria hepatica and larval forms of Taenia taeniaeformis in wild rats (R. norvegicus) captured in an urban area. Materials and methods. Two hundred and fifty-four adult specimens of R. norvegicus were collected in an urban zone of Medellín, Colombia. The livers of 54 specimens that showed macroscopic hepatic lesions during necropsy were examined by conventional histopathology.Results. The frequency of infestation with C. hepatica was 20.1% (51/254). Six livers (2.4%) were also positive for larvae of T. taeniaeformis. Livers infested with C. hepatica exhibited adult or juvenile parasites and oval eggs with bipolar opercula, and were associated with mild to moderate multifocal granulomatous hepatitis with leucocyte infiltrate. Granulomatous lesions and calcified residual fibroses

show abstract

Prevalence of Leptospira spp. in Urban Rodents from a Groceries Trade Center of Medellín, Colombia

Cited by 55 publications

References 13 publications

Alteraciones histopatológicas en ratas naturalmente infectadas con Leptospira

Alteraciones histopatológicas en ratas naturalmente infectadas con Leptospira

A retrospective analysis of the leptospirosis research in Colombia

Rattus norvegicus como indicador de la circulación de Capillaria hepatica y Taenia taeniaeformis en la Plaza Minorista de Medellín, Colombia

Contact Info

Product

Resources

About