Prevalence of metallo-β-lactamase among Pseudomonas aeruginosa and Acinetobacter baumannii isolated from burn wounds and in vitro activities of antibiotic combinations against these isolates

Altoparlak, Ülkü; Aktaş, Ferda; Çelebi, Demet; Özkurt, Zülal; Akçay, Müfide Nuran

doi:10.1016/j.burns.2005.02.017

Cited by 44 publications

(31 citation statements)

References 13 publications

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“…Additionally, in these studies, the prevalence rates of efflux pumps overexpression, including MexABOprM, MexEF-OprN, MexXY-OprM, and MexCDOprJ were found to be 21.1-57.9%, 3.5-26%, 15.8-37.5%, and 2.6-31.3% respectively [7,[21][22][23][24][25]. In our study, the prevalence of carbapenem resistance mechanisms in P. aeruginosa was comparable with that in previous studies, except for the prevalence of overexpression of MexXY-OprM, which was lower.…”

Section: Discussionmentioning

confidence: 99%

Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates

Müderris

Durmaz

Özdem

et al. 2018

J Infect Dev Ctries

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Introduction: In recent years, the prevalence of multidrug-resistant P. aeruginosa has remarkably increased. Thus, we wanted to investigate the carbapenem resistance mechanisms and clonal relationship among 80 carbapenem-resistant P. aeruginosa strains. Methodology: Carbapenemase production was detected using the Modified Hodge Test (MHT), EDTA combined disc method (ECD), and PCR. Expression levels of efflux and porin genes were mesured by real-time reverse transcription PCR. Clonal relationship of the isolates was investigated by pulsed-field gel electrophoresis (PFGE). Results: Carbapenemase production was detected in 7.5% of the isolates with MHT/ECD tests and in 11.3% of the isolates with PCR. Although the specificity of MHT/ECD was high, the sensitivitivity was low. oprD downregulation and mexB, mexY, and mexD overexpression were demonstrated in 55%, 16.3%, 2.5%, and 2.5% of the isolates, respectively. Multiple carbapenem resistance mechanisms were found in nearly a quarter of the isolates. PFGE typing of the 80 P. aeruginosa isolates yielded 61 different patterns. A total of 29 isolates (36.3%) were classified in 10 clusters, containing 2 to 7 strains. We could not find a strict relationship between PFGE profile and carbapenem resistance mechanisms. Conclusions: Although oprD downregulation and MexAB-OprM overexpression were the most common mechanisms, carbapenem resistance was associated with multiple mechanisms in the study. MHT/ECD tests should not be used alone for investigation of carbapenemase production in P. aeruginosa. Rapid tests with high sensitivity and specificity should be developed for the detection of carbapenemase production in P. aeruginosa.

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Section: Discussionmentioning

confidence: 99%

Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates

Müderris

Durmaz

Özdem

et al. 2018

J Infect Dev Ctries

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“…In the future, there may be serious problems in the treatment of infections caused by these bacteria. Since the resistance to other classes of antibiotics, such as aminoglycosides and fluoroquinolones, is often related with ESBL and AmpC production (37,38) therefore, ESBL-producing isolates must be recognized in community and hospitals for appropriate treatment and prevent the outbreak of ESBL-producing bacteria. It is important to identify infections caused by ESBL-producing bacteria in the community and hospitals for appropriate treatment and prevention outbreak of ESBLs and AmpC producing isolates.…”

Section: Resultsmentioning

confidence: 99%

Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of Escherichia coli Recovered from Patients with Urinary Tract Infections in Kerman, Iran

Koshesh

Mansouri

Hashemizadeh

et al. 2016

Arch Pediatr Infect Dis

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Background: Escherichia coli is the main causative pathogen in urinary tract infections (UTIs). Antibiotic resistance in this bacterium is an important problem in public health. Objectives: The aim of this study was to identify the blaTEM, blaSHV, blaOXA, and blaPER genes and AmpC-β-lactamase in clinical isolates of E. coli recovered from patients with UTIs in Kerman, Iran. Methods: E. coli isolates (N = 105) were analyzed for their antibiotic susceptibility with the disk diffusion method. ESBL and AmpCproducing isolates were detected using phenotypic methods. PCR was used to identify the blaTEM, blaSHV, blaOXA and blaPER genes in ESBL and AmpC-positive isolates. Results: More than 50% of the isolates were multi-drug resistant. The prevalence of ESBLs, AmpC-β-lactamase, blaTEM and blaOXA in the inpatient isolates was 37.2%, 2%, 37.2% and 5.8%, respectively. Further, the prevalence of ESBLs, blaTEM, blaSHV and blaOXA in the outpatient isolates was 42.5%, 24%, 5.5% and 1.8%, respectively. Conclusions: The prevalence of ESBL-producing E. coli strains in the community (outpatients) is higher than that in inpatients in Kerman, Iran. An outbreak of ESBL-producing isolates in the community can be a serious problem for public health, as resistance to other classes of antibiotics such as aminoglycoside and fluoroquinolones is often related with ESBL and AmpC production, therefore, detection of ESBL and AmpC-producing isolates in the community and hospitals is very important for the treatment and prevention of such isolates.

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“…37 However, in 2005, a study by Altoparlak et al found that 33% of imipenem-resistant isolates of A. baumannii from a burn wound unit in a hospital in Turkey were MBL producers. 38 In 2011, Peymani et al reported the phenotypic detection of MBL producers in 49% (31/63) of A. baumannii isolates in a tertiary hospital in Iran using the MBL E-test. 39 The VIM gene was detected in 29% (9/31) and the IMP gene in 61% (19/31) of the MBL-producing isolates, highlighting the increase in MBL genes in Iran.…”

mentioning

confidence: 99%

Prevalence of carbapenem resistance genes inAcinetobacter baumanniiisolated from clinical specimens obtained from an academic hospital in South Africa

Kock

Bellomo

Storm

et al. 2013

Southern African Journal of Epidemiology and Infection

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Original Research: Prevalence of carbapenem resistance genes in Acinetobacter baumannii isolated from clinical specimens Acinetobacter baumannii is an important cause of hospital-acquired infections. The occurrence of carbapenem resistance that is caused by the carbapenem-hydrolysing class D β-lactamases and the metallo-β-lactamases (MBLs) limits the range of therapeutic alternatives in treating A. baumannii infections. In this study, two multiplex polymerase chain reactions were performed to screen for both carbapenem-hydrolysing class D β-lactamases and MBL genes in 97 clinical isolates of A. baumannii. Oxacillinase (OXA)-51 had a prevalence of 83% (81/97), and OXA-23 had a prevalence of 59% (57/97). One isolate was positive for an MBL [Verona integron-encoded metallo β-lactamases (VIM)]. Therefore, continuous surveillance and monitoring of A. baumannii is crucial because of the high prevalence of antibiotic resistance genes.Peer reviewed.

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Prevalence of metallo-β-lactamase among Pseudomonas aeruginosa and Acinetobacter baumannii isolated from burn wounds and in vitro activities of antibiotic combinations against these isolates

Cited by 44 publications

References 13 publications

Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates

Role of efflux pump and OprD porin expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates

Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of Escherichia coli Recovered from Patients with Urinary Tract Infections in Kerman, Iran

Prevalence of carbapenem resistance genes inAcinetobacter baumanniiisolated from clinical specimens obtained from an academic hospital in South Africa

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