Prevalence of Multidrug Resistant Escherichia Coli in Pigeon in Mymensingh, Bangladesh

Dey, Rubel Kanti; Khatun, Mst. Minara; Islam, Ariful; Hosain, Sahadat

doi:10.3329/mh.v2i1.17254

Cited by 9 publications

(11 citation statements)

References 13 publications

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“…Forty samples were analyzed in this study and the prevalence was found to be 52.5% (21/40) for E. coli isolates (Tables -3 and 4). The prevalence is slightly lower than the prevalence reported by Dey et al [31]. In that study, 112 samples obtained from seemingly healthy pigeons of different places of the Mymensingh district were analyzed and 69.64% prevalence of E. coli was reported.…”

Section: Discussionmentioning

confidence: 69%

Multidrug-resistant Escherichia coli and Salmonella spp. isolated from pigeons

et al. 2020

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Background and Aim: Pigeon rearing has been gaining popularity for recent years. They are reared remarkably very close to the house of the owner. This activity, therefore, may pose potential threats for humans as well as other animals as pigeons may carry and spread different pathogens including drug-resistant bacteria. This study was conducted to explore the prevalence of Escherichia coli and Salmonella spp. as well as their antibiogram profile along with an association analysis. Materials and Methods: Forty swab samples were collected from 20 pigeons during the study. E. coli and Salmonella spp. were isolated and identified on various types of agars, including MacConkey, Eosin methylene blue, Brilliant green, and Salmonella-Shigella agar. Biochemical tests such as the carbohydrate fermentation test, the triple sugar iron agar slant reaction, the indole test, the methyl red test, the catalase test, as well as the Voges–Proskauer test were also performed. Besides, the presence of E. coli was further confirmed by polymerase chain reaction (PCR). Moreover, antimicrobial susceptibility testing of the isolates was performed against nine antibiotics from seven classes on the Mueller-Hinton agar based on the Kirby–Bauer disk diffusion method. Results: The overall prevalence of E. coli and Salmonella spp. was 52.5 and 27.5%, respectively. The prevalence of the pathogenic E. coli was 61.90%. The antibiogram profile of 21 E. coli as well as 11 Salmonella spp. revealed that all isolates, except one, were resistant to one to six antibiotics. Around 61.90%, 71.43%, 23.81%, 61.90%, 23.81%, 19.05%, and 52.38% of E. coli showed resistance against amoxicillin, ampicillin, azithromycin, erythromycin, nalidixic acid, gentamicin, and tetracycline, respectively. Furthermore, E. coli resistance was not observed in case of ciprofloxacin and levofloxacin. Similarly, around 36.36%, 27.27%, 27.27%, 45.45%, 81.82%, 100%, and 18.18% of the Salmonella spp. showed resistance against amoxicillin, ampicillin, azithromycin, erythromycin, nalidixic acid, tetracycline, and levofloxacin, respectively. However, all Salmonella spp. (100%) were found to show sensitivity against ciprofloxacin and gentamicin. Multidrug-resistant (MDR) E. coli (23.80%) and Salmonella spp. (54.54%) were also isolated. Furthermore, both positive (odds ratio [OR] >1) and negative (OR <1) drug resistance associations, with a higher frequency of positive associations, were found in E. coli. A significant positive association was observed between ampicillin and amoxicillin (OR: 81.67, 95% confidence interval: 2.73-2447.57, p=0.01). Conclusion: Pigeon carrying MDR E. coli and Salmonella spp. may contribute to the transmission and spread of these microorganisms. Therefore, strict hygienic measures should be taken during the farming of pigeons to decrease the potential transmission of E. coli and Salmonella spp. from pigeon to humans as well as other animals. So far, this is the first report of the PCR-based identification of pathogenic E. coli from pigeons in Bangladesh.

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Section: Discussionmentioning

confidence: 69%

Multidrug-resistant Escherichia coli and Salmonella spp. isolated from pigeons

et al. 2020

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“…A total of eight ABR bacterial species were reported in layer, broiler, backyard poultry, ducks, pigeons, and quails in nine different districts of Bangladesh (Table-1) [8,15,16,22,[27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44]. ABR Escherichia coli was identified in different types of birds from both conventional and unconventional farming systems in different areas of Bangladesh.…”

Section: Antibiotic-resistant Bacteria Reported In the Poultry Sectormentioning

confidence: 99%

“…These extended-spectrum β-lactamases (ESBL) producing E. coli from backyard poultry carried mcr-1 plasmid and resistant genes bla CTX-M-group-1 , bla TEM -1 (ESBL), qnrB (fluoroquinolone), and rmtB (aminoglycoside) [15]. MDR and ESBL-producing E. coli was also found prevalent in ducks, geese, quails, and pigeons from unconventional commercial farms and LBMs [31][32][33]. The E. coli isolates from the birds of unconventional farms were resistant against tetracycline (90%), amoxicillin (70%), sulfamethoxazole (90%), and other antibiotics commonly used in broiler and layer farms.…”

Section: Antibiotic-resistant Bacteria Reported In the Poultry Sectormentioning

confidence: 99%

Antimicrobial resistance situation in animal health of Bangladesh

et al. 2020

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Antimicrobial resistance (AMR) is a crucial multifactorial and complex global problem and Bangladesh poses a regional and global threat with a high degree of antibiotic resistance. Although the routine application of antimicrobials in the livestock industry has largely contributed to the health and productivity, it correspondingly plays a significant role in the evolution of different pathogenic bacterial strains having multidrug resistance (MDR) properties. Bangladesh is implementing the National Action Plan (NAP) for containing AMR in human, animal, and environment sectors through "One Health" approach where the Department of Livestock Services (DLS) is the mandated body to implement NAP strategies in the animal health sector of the country. This review presents a "snapshot" of the predisposing factors, and current situations of AMR along with the weakness and strength of DLS to contain the problem in animal farming practices in Bangladesh. In the present review, resistance monitoring data and risk assessment identified several direct and/or indirect predisposing factors to be potentially associated with AMR development in the animal health sector of Bangladesh. The predisposing factors are inadequate veterinary healthcare, monitoring and regulatory services, intervention of excessive informal animal health service providers, and farmers' knowledge gap on drugs, and AMR which have resulted in the misuse and overuse of antibiotics, ultimate in the evolution of antibiotic-resistant bacteria and genes in all types of animal farming settings of Bangladesh. MDR bacteria with extreme resistance against antibiotics recommended to use in both animals and humans have been reported and been being a potential public health hazard in Bangladesh. Execution of extensive AMR surveillance in veterinary practices and awareness-building programs for stakeholders along with the strengthening of the capacity of DLS are recommended for effective containment of AMR emergence and dissemination in the animal health sector of Bangladesh.

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“…Studies reported that the intestinal microbiota of wild birds and urban pigeons includes STEC and EPEC, as well as multidrug-resistant (MDR) strains (Silva et al, 2009;Askari Badouei et al, 2014;Chandran and Mazumder, 2014;Dey et al, 2014;Gargiulo et al, 2014;Chiacchio et al, 2016). Additionally, putative adhesins were detected in STEC and EPEC strains from humans and animals worldwide, including wild birds (Afset et al, 2006;Borges et al, 2012;Cooley et al, 2013;Beraldo et al, 2014;Miko et al, 2014;Akiyama et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Wild birds and urban pigeons as reservoirs for diarrheagenic Escherichia coli with zoonotic potential

et al. 2017

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In order to describe the role of wild birds and pigeons in the transmission of shiga toxigenic Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) to humans and other animals, samples were collected from cloacae and oropharynx of free-living wild birds and free-living pigeons. Two STEC (0.8%) and five EPEC strains (2.0%) were isolated from wild birds and four EPEC strains (2.0%) were recovered from pigeons. Serogroups, sequence types (STs) and virulence genes, such as saa, iha, lpfA , ehxA, espA, nleB and nleE, detected in this study had already been implicated in human and animal diseases. Multidrug resistance (MDR) was found in 25.0% of the pigeon strains and in 57.0% of the wild bird strains; the wild birds also yielded one isolate carrying extended-spectrum β-lactamases (ESBLs) gene bla. The high variability shown by PFGE demonstrates that there are no prevalent E. coli clones from these avian hosts. Wild birds and pigeons could act as carriers of multidrug-resistant STEC and EPEC and therefore may constitute a considerable hazard to human and animal health by transmission of these strains to the environment.

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Prevalence of Multidrug Resistant Escherichia Coli in Pigeon in Mymensingh, Bangladesh

Cited by 9 publications

References 13 publications

Multidrug-resistant Escherichia coli and Salmonella spp. isolated from pigeons

Multidrug-resistant Escherichia coli and Salmonella spp. isolated from pigeons

Antimicrobial resistance situation in animal health of Bangladesh

Wild birds and urban pigeons as reservoirs for diarrheagenic Escherichia coli with zoonotic potential

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