Prevalence of Mycoplasma hominis and Ureaplasma spp. in Routine Gynecological Care in Sao Paulo City, Brazil

Milanezi, Fernanda; Falconi, Ariane; Beatriz, Schnabel; Ricardi, Luana R.; Monfredini, Priscilla M.; Ziliotto, Alline T.; Lopes, Vanessa F.; Machado, Silvana; Oliveira, Marinelia J.; Centrone, Cristiane C.; Nakano, Viviane

doi:10.5812/archcid.36668

Cited by 2 publications

(3 citation statements)

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“…However, this method is time-consuming (needs 3-6 days), difficult, and expensive and requires special handling and trained persons for colony identification, which depends on the expertise and the personal naked eye power. 2,21 On the other hand, PCR assay was previously compared with specific culture method for detection of mycoplasmas, and it showed good sensitivity, specificity, as well as positive and negative predictive values (87%, 96%, 94%, and 93%, respectively). 28 All these data supported the use of mPCR assay as the gold comparable technique to measure the accuracy of the commercial MSP kit in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…However, isolation by specific culture media remains the gold standard method for detection of mycoplasmas. However, this method is time-consuming (needs 3–6 days), difficult, and expensive and requires special handling and trained persons for colony identification, which depends on the expertise and the personal naked eye power 2,21 . On the other hand, PCR assay was previously compared with specific culture method for detection of mycoplasmas, and it showed good sensitivity, specificity, as well as positive and negative predictive values (87%, 96%, 94%, and 93%, respectively) 28 .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, they cannot be detected by light microscopy. In addition, their growth is slow and fastidious, and they need special enriched media supplemented with amino acids and fatty acids 1,2 . Unlike the genus Mycoplasma , Ureaplasma hydrolyze urea and use it as a metabolic substrate to generate adenosine triphosphate, and they cannot use arginine because they lack the arginine deiminase enzyme of Mycoplasma 3 …”

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confidence: 99%

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Rapid Detection of Urinary Ureaplasma urealyticum and Mycoplasma hominis Isolated from Pregnant Women and Their Antibiotic Susceptibility Profile

Elkolaly¹,

Amin²,

Mohamed³

et al. 2021

Infect Dis Clin Pract

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Background: Urinary tract infection is responsible for serious complications in pregnant women. Among different uropathogens, Ureaplasma urealyticum and Mycoplasma hominis are implicated in several urogenital infections. These organisms are not easily diagnosed by routine cultures and cannot be treated by common safe antibiotics used in pregnancy. This is the first study that aimed to rapidly detect these organisms in "urine of pregnant" women and determine their antibiotic susceptibility.Methods: A total of 1500 pregnant women were included, 750 symptomatic patients and 750 controls (apparently healthy). Urine samples were taken aseptically from all subjects and analyzed using the Mycoplasma System Plus (MSP) kit. The specimens were further subjected to multiplex polymerase chain reaction (PCR) for the molecular confirmation.Results: Of 750 urine specimens of the patients group, 223 specimens showed sterile pyuria suggesting the presence of mycoplasmas, whereas sterile pyuria was found in only 65 specimens of the control group. Of the 223 specimens of the patients group, 32 (14.3%) had U. urealyticum by MSP kit, 21 (9.4%) had M. hominis, 14 (6.3%) showed mixed coinfection, and 156 (70%) were negative. Seven (10.8%) of 65 specimens of the control group detected U. urealyticum. Conclusions:There was a good agreement between the MSP kit and the multiplex PCR. The MSP kit was an easy and rapid alternative to the complicated conventional culture method for the detection of urinary mycoplasmas. Doxycycline and minocycline had the highest sensitivity rates; however, clindamycin would be the first choice for empirical treatment of pregnant women.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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