Prevalence of mycoplasmas in eggs from birds of prey using culture and a genus-specific mycoplasma polymerase chain reaction

An analysis of the biofilms adhered to framework alloys using in vitro denture plaque models

“…Qualitative PCR was carried out according to the previous studies [28][29][30][31][32][33][34][35][36][37][38][39][40] using DNA templates extracted from the biofilms on five alloys, resin and the individual saliva samples (N=5). The primers used were for the genera of Actinomyces 28) , Burkholderia 29) , Fusobacterium 30) , Haemophilus 31) , Mycoplasma 32) , Neisseria 33) , Peptostreptococcus 34) , Pseudomonas 35) , Streptococcus 36) , Staphylococcus 37) , and Candida 38) and for the species of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola, Tannerella forsythia 39) and S. mutans 40) .…”

Conjunctivitis, rhinitis, and sinusitis in cliff swallows (Petrochelidon pyrrhonota) found in association withMycoplasma sturniinfection and cryptosporidiosis

“…The same procedure was used for DNA extraction of mycoplasma isolates in broth cultures made from both sample submissions. Conventional PCR for M. gallisepticum was performed using primers to the mgc2 surface protein gene (Hong et al, 2005), and for mycoplasmas using primers (GPF/ MGSO) to the 16S rRNA gene, reported to detect Mycoplasma species, Acholeplasma species (Lierz et al, 2007) and Ureaplasma species (D. H. Ley, data not shown). Amplified PCR products were separated in 2% agarose gels and visualized by ethidium bromide staining and ultraviolet transillumination.…”

Pathogenicity ofMycoplasma lipofaciensstrain ML64 for turkey embryos

“…The fifth single-colony subculture of mycoplasma strain ML64 that had been used for the immunobinding assay and DNA sequencing (GenBank accession number DQ653410) for identification as M. lipofaciens (Lierz et al, 2007a) was cultured (24 h, 378C, 5% CO 2 ) in liquid mycoplasma media as described by Bradbury (1998) but without thallium acetate. The number of colonyforming units (CFU) was determined according to standard methods (Albers & Fletcher, 1982) and the suspension was diluted with sterile mycoplasma broth to the required dose (see below) directly prior to inoculation and without additional incubation.…”

“…After a final washing, positive colonies appear blue while negative colonies remain white. Samples of vitteline membrane were also taken to detect Mycoplasma genus-specific DNA using a polymerase chain reaction (PCR) described by Lierz et al (2007a). The PCR samples were first stored at (208C and tested only if isolation from the embryo was negative.…”

“…Subsequently, isolation of M. lipofaciens was reported from the chicken, turkey and duck (Bencina et al, 1987). Recently, Lierz et al (2007a) isolated M. lipofaciens from an egg of an imprinted 4-year-old Northern Goshawk (Accipiter gentilis). Identification was carried out by immunobinding assay as well as sequencing a fragment (928 base pairs) of the 16S rRNA gene (GenBank accession number DQ653410).…”