Prevalence of Nasal Carriage of Staphylococcus aureus and its Antibiotic Susceptibility among Healthcare Workers (HCWs) in Ukraine

Salmanov, Aidyn G; Verner, O.M.; Shelkova, N.G.; Nicolska, O.I.; Blahodatny, V.M.; Slepova, L.F.

doi:10.31405/ijap.2-1.18.03

Cited by 3 publications

(1 citation statement)

References 26 publications

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“…The frequency of MRSA carriage also varied according to the department/ward. The highest prevalence of nasal carriage of MRSA was in the surgical wards [11]. However, the burden of MRSA colonisation seems to extend beyond the hospitals to Dentistry setting and outpatient Otorinolaryngology care.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Nasal Carriage of Methicillin-Resistant Staphylococcus Aureus Among Healthcare Workers in the Departments of Otorinolaryngology and Dentistry in Kyiv, Ukraine

et al. 2020

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The aim: To obtain the first estimates of the current prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) in the departments of Otorinolaryngology and Dentistry and to determine of genes virulence factors (Panton Valentine Leukocidine (PVL) genes). Materials and methods: We performed a multicenter cross-sectional study. The susceptibility to antibiotics was determined by disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing. The virulence factor encoding genes, mecA, lukS-lukF, were detected by Polymerase Chain Reaction (PCR). Results: Incidence rate of S. aureus nasal carriage among HCWs was 36.2%, whereas MRSA carriage was 17%. Prevalence of MRSA carriage rate was 34.9% in Otorhinolaryngology departments and 9.7% in Dentistry. PCR testing confirmed that all MRSA strains were mecA gene-positive. The virulence factor encoding genes were detected in 82.3% of the S. aureus isolates from HCWs. Among S.aureus, the lukS-lukF genes were detected in over 59% of the strains. The lukS-lukF genes were detected in 55.5% of MRSA and in 58.9% of MSSA strains. LukS-lukF genes were most commonly co-present in MRSA strains. No significant difference was detected between the occurrences of lukS-lukF genes (P > 0.05). Conclusions: Personnell in otorhinolaryngology and dentistry departments have a high rate of nasal colonization of MRSA. This carrier state may be an important risk factor for transmission MRSA from physicians and nurses to patients and vice-versa. Screening for MRSA nasal carriage of HCWs is a key element in enabling infection control measures and early therapeutic decisions.

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Section: Introductionmentioning

confidence: 99%

Prevalence of Nasal Carriage of Methicillin-Resistant Staphylococcus Aureus Among Healthcare Workers in the Departments of Otorinolaryngology and Dentistry in Kyiv, Ukraine

et al. 2020

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Antimicrobial Resistance of Staphylococcus Aureus Causing of Surgical Site Infections in Ear, Nose And Throat Surgery

Salmanov

Shkorbotun

2019

Wiad Lek

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Introduction: Staphylococcus aureus is one of the major pathogens that causes of surgical site infection (SSI). Scant information is available on the occurrence and antimicrobial susceptibility of S. aureus in patients with SSI in Ear, Nose and Throat (ENT) surgery. The aim: To assess the activity of antimicrobials against S.aureus, isolated from patients with SSI by the ENT departments of Kyiv hospitals. Materials and methods: A total of 516 S. aureus isolates from of patients with SSI in ENT surgery. Antimicrobial susceptibility of S. aureus were determined, using automated microbiology analyzer. Some antimicrobial susceptibility test used Kirby – Bauer antibiotic testing. Interpretative criteria were those suggested by the Clinical and Laboratory Standards Institute (CLSI). Results: The most active antibiotics found in the study were linezolid and tigecycline, showing growth inhibition of 100% strains tested. Susceptibility to the other antimicrobials was also on a high level: 98,4% of strains were found susceptible to nitrofurantoin, 98.1% – to trimethoprim/sulphamethoxazole, 97.6% – to fusidic acid, 97.1% – to mupirocin, 95.9% – to teicoplanin, 94.7% – to vancomycin and fosfomicin, 90.6% – to moxifloxacin, 89.1% – to tobramycin, 87.3% – to gentamycin. Susceptibility to rifampicin (85.5%), cefoxitin (84.6%), levofloxacin (84.3%), erythromycin (82.6%), tetracycline (76.3%), and clindamycin (75.4%) was observed to be some lower. Resistance to oxacyllin S.aureus (MRSA) came up to 21.1%. Conclusions: S. aureus in ENT departments to be a serious therapeutic and epidemiologic problem. The constant monitoring of antimicrobials resistance in every hospital is required. Antibiotics application tactics should be determined in accordance with the local data of resistance to them.

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Molecular Epidemiology of the Transmission of Methicillin-Resistant Staphylococcus Aureus in Kyiv Acute Care Hospitals, Ukraine

Salmanov

Shchehlov²,

Shkorbotun

et al. 2022

Wiad Lek

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The aim: To evaluate the potential of transmission of methicillin-resistance Staphylococcus aureus (MRSA) in Ukrainian acute care hospitals. Materials and methods: We performed a multicenter cross-sectional study. Definitions of HAI were used from the CDC/ NHSN. The susceptibility to antibiotics was determined by disk diffusion method according to the EUCAST. The cefoxitin-resistant isolates S.aureus were analyzed for the presence of the mecA gene and femA endogenous control gene using PCR. The virulence factor encoding genes (lukS-PV and lukF-PV) were detected by PCR. Results: Of 2,421 patients with HAIs caused S.aureus included in the study, 28.7% patients had MRSA. Prevalence of nasal carriage rate of MRSA among healthcare workers (HCWs) was 33.3%. MRSA contamination of hands and uniforms/gowns of HCW were 32.2% and 29.7%, respectively. MRSA contamination in the inanimate environment surfaces in near- and extended patients areas were 26.9%. The predominant MRSA contamination in hospital environment surfaces were: room inner door knob (32.8%), bed rails (28.9%), room light switch (28.9%), chair (27.9%), bedside table handle (20.6%), bedside table (20.5%), and tray table (13.7%). The PVL gene was present in 38.7% of MRSA strains, isolated from patients with HAIs and in 55.7% of MRSA, isolated from environment surfaces in patient area. In addition, the PVL genes were detected in over 56.3% of MRSA isolated from HCWs carrier. Conclusions: The majority of MRSA is acquired during hospitalization. Environmental surfaces may serve as potential reservoirs for nosocomial MRSA and facilitate transmissions via contact.

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Prevalence of Nasal Carriage of Staphylococcus aureus and its Antibiotic Susceptibility among Healthcare Workers (HCWs) in Ukraine

Cited by 3 publications

References 26 publications

Prevalence of Nasal Carriage of Methicillin-Resistant Staphylococcus Aureus Among Healthcare Workers in the Departments of Otorinolaryngology and Dentistry in Kyiv, Ukraine

Prevalence of Nasal Carriage of Methicillin-Resistant Staphylococcus Aureus Among Healthcare Workers in the Departments of Otorinolaryngology and Dentistry in Kyiv, Ukraine

Antimicrobial Resistance of Staphylococcus Aureus Causing of Surgical Site Infections in Ear, Nose And Throat Surgery

Molecular Epidemiology of the Transmission of Methicillin-Resistant Staphylococcus Aureus in Kyiv Acute Care Hospitals, Ukraine

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