Prevalence of Neospora caninum infection in Australian (NSW) dairy cattle estimated by a newly validated ELISA for milk

Hall, C A; Reichel, Michael P.; Ellis, John

doi:10.1016/j.vetpar.2006.06.019

Cited by 26 publications

(16 citation statements)

References 17 publications

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“…There have been a number of individual herd investigations for neosporosis reported in dairy herds in New South Wales (NSW), with seroprevalences around 10–11% (ELISA) in two of the herds and 24% (indirect‐fluorescent antibody test: IFAT) in a third herd. A study of NSW dairy cattle using a milk ELISA found 21% prevalence of N. caninum antibodies in 398 cows sampled from 203 farms, with one or more positive animals in 35% of the farms sampled . However, until our study was undertaken, there had not been a statistically designed survey of neosporosis in NSW beef herds.…”

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confidence: 89%

Neospora caninum in beef herds in New South Wales, Australia. 1: seroprevalence study

2017

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confidence: 89%

Neospora caninum in beef herds in New South Wales, Australia. 1: seroprevalence study

2017

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“…Thus, in a comparative study carried out by Bartels et al (2006), seroprevalences on European dairy farms ranged between 0.5% in Sweden and 16.2% in Spain. Prevalences have been estimated at between 14.1 and 40.4% in America (Paré et al 1996;Chi et al 2002;Moore 2005), 5.7-35.6% in Asia (Hur et al 1998;Koiwai et al 2005) and 6.0-21.1% in Oceania (Reichel 1998;Hall et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology of neosporosis in dairy cattle in Galicia (NW Spain)

et al. 2007

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This comprehensive study of neosporosis in dairy cattle in Galicia (NW Spain) included: (1) a comparative study of three serological techniques for detection of Neospora caninum antibodies (direct agglutination, enzyme-linked immunosorbent assay and indirect immunofluorescence); (2) a cross-sectional serological survey in which 276 herds and 5,196 animals were tested; (3) a study of N. caninum antibody dynamics; (4) the isolation of viable tachyzoites of N. caninum. Data were analysed to determine the risk factors associated with the infection. A total of 219 herds (79.3%) and 816 heads of cattle (15.7%) were found to be seropositive. Seropositivity was higher on farms with dogs than on farms without dogs, and there was a negative correlation between the size of the herds and seroprevalence. Co-infection with Toxoplasma gondii increased the risk of seropositivity. Cows infected with N. caninum were 5.3 times more likely to abort than non-infected cows. The dynamics study showed an increase in anti-N. caninum antibody titres during the third trimester of pregnancy. Viable tachyzoites were isolated from brain samples. These results indicate that the economic impact of N. caninum is high in Galicia, and therefore, the inclusion of control measures for neosporosis in the official control health programmes is strongly recommended.

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“…Hall et al (2006) validated this Idv ELISA for detection of N. caninum antibodies in bovine milk, optimizing cut-off values by TG-ROC analysis and obtained a 97% sensitivity and specificity, respectively. In the present study serum and milk samples were tested by the same method, Idv ELISA, but in the study of Hall et al (2006) cut-off determination for milk Idv ELISA was made in relation to serum samples tested by an Idx ELISA. Difference in method used for testing reference serum samples might explain the different optimized cut-off values reported for Idv ELISA.…”

Section: Discussionmentioning

confidence: 94%

“…In previous studies conducted in different regions of the world several ELISAs were adapted for detection of Neospora caninum antibodies in cattle using individual milk samples (Björkman et al 1996, Schares et al 2004, Hall et al 2006, bulk milk samples (Bartels et al 2005, Frossling et al 2006, Wapenaar et al 2007 or both (González-Warleta et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Comparative study for the detection of antibodies to Neospora caninum in milk and sera in dairy cattle in southern Romania

Enăchescu

Ioniţă

Mitrea

2014

Acta Parasitologica

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The study aimed to assess the within-herd Neospora caninum exposure in dairy cattle in southern Romania, based on the detection of specific antibodies in milk and serum. A total of 104 paired samples of milk and serum were collected from four dairy farms. Individual samples were analyzed for N. caninum antibodies by ELISA: IDEXX Neospora Ab (Idx) (three farms: A, B, C; n = 60) and ID-VET Lab (Idv) (farm D; n = 44). Additionally, four pooled milk samples, one per each farm (A, B, C) and a composed one (A+B+C), were analyzed with Idx ELISA. Optimized cut-off values for milk samples were determined by receiver operating characteristic (ROC) analysis, with serum results considered as true status. The agreement was expressed by K values. The overall seroprevalence of N. caninum infection was 45% in the farms tested by Idx ELISA and 56.8% in the farm tested by Idv ELISA. A good agreement between serum and milk was obtained for both ELISA kits (K = 0.72 and 0.77, respectively). The specificity and sensitivity at optimized cut-off of S/P>0.704 for Idx and S/P%>7.966% for Idv were 100% and 70.37% for Idx and 89.47% and 88% for Idv. Testing pooled milk samples, there were identified as N. caninum positive the dairy farms with a 15% or higher within-herd seroprevalence at the cut-off value of S/P>0.51. This is the first study in Romania in which milk samples were tested to determine the N. caninum infection status in dairy farms, providing a base for further researches.

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Prevalence of Neospora caninum infection in Australian (NSW) dairy cattle estimated by a newly validated ELISA for milk

Cited by 26 publications

References 17 publications

Neospora caninum in beef herds in New South Wales, Australia. 1: seroprevalence study

Neospora caninum in beef herds in New South Wales, Australia. 1: seroprevalence study

Epidemiology of neosporosis in dairy cattle in Galicia (NW Spain)

Comparative study for the detection of antibodies to Neospora caninum in milk and sera in dairy cattle in southern Romania

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