Prevalence of oral hairy leukoplakia and epithelial infection by Epstein-Barr virus in pregnant women and diabetes mellitus patients: cytopathologic and molecular study

Milagres, Adrianna; Dias, Eliane Pedra; Tavares, Débora dos Santos; Cavalcante, Roberta Miranda; Dantas, Vivian Antunes; Oliveira, Silvia Paula De; Leite, José Paulo Gagliardi

doi:10.1590/s0074-02762007000200006

Cited by 10 publications

(3 citation statements)

References 13 publications

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“…Previous literature has unveiled conflicting findings regarding the detection of EBV-1 DNA in OHL lesions. 24,25,30 One study 30 detected EBV-1 DNA in all OHL cases before topical treatment, whereas another did not find EBV-1 in all OHL samples. Moreover, a report indicated the absence of EBV-1 DNA samples in individuals with clinical evidence of OHL.…”

Section: Discussionmentioning

confidence: 99%

Identification of Epstein-Barr virus after topical treatment for oral hairy leukoplakia: A preliminary study

Nobre,

Moura,

de Arruda

et al. 2024

Int J STD AIDS

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Background This study evaluated the presence of Epstein-Barr virus type 1 (EBV-1) DNA in patients living with HIV, before and after three different topical therapy protocols for oral hairy leukoplakia (OHL). Methods The sample consisted of five patients treated with topical solution of 25% podophyllin resin; six with 25% podophyllin resin plus 5% acyclovir cream; and four with 25% podophyllin resin plus 1% penciclovir cream. DNA was extracted from OHL scrapings and amplified by the PCR using specific primers for EBV-1 (EBNA-1). Results Clinical healing of OHL lesions was observed across all treatment groups over time. At baseline, EBNA-1 was detected in all OHL lesions. After treatment, OHL samples from three patients treated with 25% podophyllin resin plus 5% acyclovir cream and from one patient treated with 25% podophyllin resin plus 1% penciclovir cream exhibited negative EBNA-1 viral gene encoding. Despite the clinical resolution of OHL, 11 patients (73.3%) showed EBNA-1 positivity immediately after the lesion disappeared. Three patients (20%) treated with podophyllin resin displayed both EBNA-1 positivity and a recurrence of OHL, in contrast to no recurrence in the other two groups. Conclusions These findings suggest potential associations between treatment formulations, EBNA-1 persistence, and the recurrence of OHL lesions.

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Section: Discussionmentioning

confidence: 99%

Identification of Epstein-Barr virus after topical treatment for oral hairy leukoplakia: A preliminary study

Nobre,

Moura,

de Arruda

et al. 2024

Int J STD AIDS

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“…EBV is yet unclear (Braz-Silva et al, 2008;Milagres et al, 2007). EBV replication increases in oral leukoplakias through mechanisms that favor its latency and persistence within the stratified squamous epithelium (Guidry et al, 2018).…”

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confidence: 99%

Human papillomavirus, Epstein–Barr virus, and Candida albicans co‐infection in oral leukoplakia with different degrees of dysplasia

Erira

Navarro

Robayo

2021

Clinical & Exp Dental Res

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Objectives: To identify human papillomavirus (HPV), Epstein-Barr virus (EBV), and Candida albicans in oral leukoplakia with different degrees of dysplasia.Materials and methods: An observational, cross-sectional, descriptive study was performed using 30 formalin-fixed and paraffin-embedded tissues from patients with clinical suspicion of leukoplakia and confirmed diagnosis of oral dysplasia. Histological analyses were performed by two pathologists (interobserver) and dysplasias were classified as mild, moderate, or severe. Conventional PCR was used to detect HPV and EBV viruses and C. albicans. To determine the association between each microorganism with different degrees of dysplasia a Chi-square test was employed. Results:The tongue was the most common site for leukoplakias (71.4%) in females with a mean age of 50 years (ranging between 30 to 50 years old; 57.1%). EBV was the most frequently detected (73.3%), followed by HPV (43.3%), mainly of type 16 (40%), and C. albicans (23.3%). Significant differences were observed between degrees of dysplasia and HPV presence (p = 0.005). In lesions positive for HPV, EBV, and C. albicans the most frequent histological changes were hyperkeratosis, irregular interpapillary ridges, and loss of basal stratum cell polarity. Conclusion:Co-infection with human papillomavirus, Epstein Barr virus, and Candida albicans in oral leukoplakia could be associated with dysplastic changes.

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“…2,3 It has also been reported in HIV-negative immunocompromised patients, such as in solid organ [4][5][6][7] or hematopoetic stem cell transplant recipients; 8 in patients with hematological malignancies, such as multiple myeloma 9 or acute myelogenic leukemia; 10,11 and in patients under systemic or topical steroid treatment. [12][13][14][15][16] Rare cases of OHL in immunocompetent healthy patients have also been reported. [16][17][18][19][20][21] The purpose of this study was to present 10 additional cases of OHL in HIV-negative patients, most of them undergoing treatment with steroids, and discuss the specific histologic features and differential diagnosis of the lesions.…”

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confidence: 99%

Oral Hairy Leukoplakia in HIV-Negative Patients: Report of 10 Cases

Piperi¹,

Omlie

Koutlas

et al. 2008

Int J Surg Pathol

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Ten cases of oral hairy leukoplakia (OHL) in HIV- negative patients are presented. Eight of the 10 patients were on steroid treatment for chronic obstructive pulmonary disease, 1 patient was on prednisone as part of a therapeutic regimen for gastrointestinal stromal tumor, and 1 patient did not have any history of immunosuppression. There were 5 men and 5 women, ages 32-79, with mean age being 61.8 years. Nine out of 10 lesions were located unilaterally on the tongue, whereas 1 lesion was located at the junction of the hard and soft palate. All lesions were described as painless, corrugated, nonremovable white plaques (leukoplakias). Histologic features were consistent with Epstein-Barr virus-associated hyperkeratosis suggestive of OHL, and confirmatory in situ hybridization was performed in all cases. Candida hyphae and spores were present in 8 cases. Pathologists should be aware of OHL presenting not only in HIV-positive and HIV-negative organ transplant recipients but also in patients receiving steroid treatment, and more important, certain histologic features should raise suspicion for such diagnosis without prior knowledge of immunosuppression.

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Prevalence of oral hairy leukoplakia and epithelial infection by Epstein-Barr virus in pregnant women and diabetes mellitus patients: cytopathologic and molecular study

Abstract: Oral hairy leukoplakia (OHL) is generally reported in patients with

Cited by 10 publications

References 13 publications

Identification of Epstein-Barr virus after topical treatment for oral hairy leukoplakia: A preliminary study

Identification of Epstein-Barr virus after topical treatment for oral hairy leukoplakia: A preliminary study

Human papillomavirus, Epstein–Barr virus, and Candida albicans co‐infection in oral leukoplakia with different degrees of dysplasia

Oral Hairy Leukoplakia in HIV-Negative Patients: Report of 10 Cases

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