Prevalence of Pathogenic Foodborne Bacteria from Beef in Retail Stalls in Kelantan

Abstract

Salmonella, E. coli O157:H7, Methicillin-resistant Staphylococcus aureus (MRSA) and Listeria monocytogenes are common pathogenic foodborne bacteria causing foodborne illness in humans. The objective of this study was to determine the prevalence of those bacteria in beef from retail stalls in Kelantan. Beef samples were collected from retail stalls (roadside stalls and wet markets) and processed for the isolation and identification of Salmonella, E. coli O157:H7, MRSA and L. monocytogenes. Out of 25 beef samples from retail stalls, 11 (44%) were positive for Salmonella, 11 (44%) positive for E. coli and 8 (32%) positive for S. aureus. None of the beef samples contained E. coli O157:H7, MRSA and L. monocytogenes. Of 11 Salmonella isolated, the most common were S. mbandaka (46%), S. albany (36%) followed by S. weltevreden (18%). In this study, Salmonella, E. coli and S. aureus were found to be the most common foodborne bacteria from beef in retail stalls in Kelantan. Therefore, consumers have to practice a good safety foodhandling during purchasing, transporting and preparing the beef in the kitchen to prevent the risk of food poisoning.

Nontyphoid Salmonella carriage, serovar profile and antimicrobial resistance phenotypes in slaughter cattle

“…Unusually, the other serovar isolated from FC samples was S . Albany, and our detailed literature search about this serovar revealed its rare reporting in cattle‐related studies as: in bovine meat in Malaysia by Fauzi et al () and, as a jejunum isolate in USA by Goodman et al (). There is no evidence until this time linking the presence of Albany in cattle to a human salmonellosis case, but our detection of S .…”

“…In the EU summary report, Salmonella prevalence in slaughterhouse bovine carcass swabs from 12 EU countries was 0%, while 3 countries as Denmark, Czech Republic, and Spain indicated the presence of this pathogen as 0.08%, 0.6%, and 2.13%, respectively (EFSA & ECDC, ). Worldwide, there are studies from U.S. (Schmidt et al, ) with lower prevalence rate as 0% in 74 carcass samples collected from three different plants; a similar rate as 0.93% in one out of 108 carcasses by Loiko et al () from Brazil; and a higher rate as 44% in 11 out of 25 carcasses by Fauzi, Arshad, Ruhil, and Al‐Sultan () from Malaysia. As an important sample type in determining NTS carriage in slaughter cattle, 1% of the FC samples in this study were determined to harbor the pathogen (Table ).…”

“…, Hadimli et al (2017),Rene et al (2017),Rizzi (2017) reporting the presence of this serovar within their FC isolates, to the best of our knowledge, this is the first study indicating the presence of S. Enteritidis in slaughter cattle MLNs. Unusually, the other serovar isolated from FC samples was S. Albany, and our detailed literature search about this serovar revealed its rare reporting in cattle-related studies as: in bovine meat in Malaysia byFauzi et al (2016) and, as a jejunum isolate in USA byGoodman et al (2017). There is no evidence until this time linking the presence of Albany in cattle to a human salmonellosis case, but our detection of S. Albany as one of the serovars in FC, thereof its occurrence in cattle is worth mentioning as an epidemiological contribution…”

Impact of hygiene and sanitation in ruminant slaughterhouses on the bacterial contamination of meat in Central Java Province, Indonesia

“… shows the level of S. aureus contamination observed in this study. The previous investigations in East Java, Indonesia, Ethiopia, and Malaysia recorded S. aureus contamination levels of 20%, 22.5%, and 32%, respectively[18,26,27]. Humans can be a…”