Prevalence of Pathogenic
            <i>Yersinia enterocolitica</i>
            Strains in Pigs in the United States

Bhaduri, Saumya; Wesley, Irene V.; Bush, Eric J.

doi:10.1128/aem.71.11.7117-7121.2005

Cited by 95 publications

(68 citation statements)

References 15 publications

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“…The primers and probe for the ail gene target (Table 1) were designed by Bhaduri et al (2005) (the authors were contacted to obtain the correct sequence for the ail reverse primer). The yadA primers and probe (Table 1) week and when possible following heavy rainfall and spring snow melt events, and were collected 2 -3 m from the river's edge and 10 -20 cm below the surface at a fast flowing area.…”

Section: Quantitative Pcr Assaysmentioning

confidence: 99%

The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes

Cheyne

Dyke

Anderson

et al. 2009

Journal of Water and Health

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Yersinia enterocolitica has been detected in surface water, and drinking untreated water is a risk factor for infection. PCR-based methods have been used to detect Y. enterocolitica in various sample types, but quantitative studies have not been conducted in water. In this study, quantitative PCR (qPCR)-based methods targeting the Yersinia virulence genes ail and yadA were used to survey the Grand River watershed in southern Ontario, Canada. Initial testing of reference strains showed that ail and yadA PCR assays were specific for pathogenic biotypes of Y. enterocolitica; however the genes were also detected in one clinical Yersinia intermedia isolate. A survey of surface water from the Grand River watershed showed that both genes were detected at five sampling locations, with the ail and yadA genes detected in 38 and 21% of samples, respectively. Both genes were detected more frequently at colder water temperatures.A screening of Yersinia strains isolated from the watershed showed that the ail gene was detected in three Y. enterocolitica 1A/O:5 isolates. Results of this study show that Yersinia virulence genes were commonly detected in a watershed used as a source of drinking water, and that the occurrence of these genes was seasonal.

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Section: Quantitative Pcr Assaysmentioning

confidence: 99%

The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes

Cheyne

Dyke

Anderson

et al. 2009

Journal of Water and Health

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“…To enrich for cultivation of Y. enterocolitica, 1 g of feces was placed in 9 ml of 0.1% peptone water and homogenized in a StomacherH laboratory blender (Metrohm USA, Riverview, Florida, USA) for 30 sec, as described by Bhaduri et al (2005). One milliliter of the homogenized mixture was added to 9 ml of Irgasan broth, mixed by inverting, and incubated at room temperature for 48 hr, as previously described (De Zutter et al, 1994).…”

Section: Enrichment and Culture To Detect Yersiniamentioning

confidence: 99%

“…After incubation in enrichment broth, tubes were inverted several times and centrifuged for 1 min at 430 3 G to remove large particulates. The remaining suspension was then plated on Cefsulodin-Irgasan Novobiocin/Yersinia-selective agar (CIN/YSA) plates, as described by Bhaduri (2005), with the addition of 0.028 g of CaCl 2 per liter of agar medium as described by Li et al (1998). The plates were incubated for 24 hr at 28 C, then checked for colony growth.…”

Section: Enrichment and Culture To Detect Yersiniamentioning

confidence: 99%

Yersinia Enterocolitica: An Unlikely Cause of Positive Brucellosis Tests in Greater Yellowstone Ecosystem Bison (Bison Bison)

See

Dauwalter

Almendra

et al. 2012

Journal of Wildlife Diseases

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ABSTRACT:Yersinia enterocolitica serotype O:9 has identical O-antigens to those of Brucella abortus and has apparently caused false-positive reactions in numerous brucellosis serologic tests in elk (Cervus canadensis) from southwest Montana. We investigated whether a similar phenomenon was occurring in brucellosis antibody-positive bison (Bison bison) using Y. enterocolitica culturing techniques and multiplex PCR of four diagnostic loci. Feces from 53 Yellowstone bison culled from the population and 113 free-roaming bison from throughout the Greater Yellowstone Ecosystem (GYE) were tested. Yersinia enterocolitica O:9 was not detected in any of 53 the bison samples collected at slaughter facilities or in any of the 113 fecal samples from free-ranging bison. One other Y. enterocolitica serotype was isolated; however, it is not known to cause cross-reaction on B. abortus serologic assays because it lacks the perosamine synthetase gene and thus the O-antigens. These findings suggest that Y. enterocolitica O:9 cross-reactivity with B. abortus antigens is unlikely to have been a cause of false-positive serology tests in GYE bison and that Y. enterocolitica prevalence was low in bison in the GYE during this study.

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“…Indeed, approximately 90% of all reported cases originate from food/ water sources. 4 Prevention is further complicated as Y. enterocolitica can grow at 4 o C and thus refrigeration does not provide sufficient protection from illness. 5 Though rarely life-threatening, the economic drain caused through temporary incapacitation from yersiniosis means that the probing for effective treatment strategies is of particular importance.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Pharmacognostic Potential of Indian Terminalia Spp. in the Treatment and Prevention of Yersiniosis

Wright¹,

Greene²,

Cock³

2017

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Introduction: Yersinia enterocolitica is a major cause of food poisoning through contaminated meat products, causing the acute gastrointestinal disease yersiniosis. Many Terminalia spp. have documented therapeutic properties as general antiseptics, inhibiting the growth of a wide variety of bacterial species. Despite this, Indian Terminalia spp. extracts have not been tested for the ability to inhibit the growth of Y. enterocolitica. Methods: T. arjuna, T. catappa and T. chebula extracts were extracted by maceration and the extracts were investigated by disc diffusion assay for growth inhibitory activity against a clinical strain of Y. enterocolitica. The MIC values of the extracts were determined to quantify and compare their efficacies. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results: T. chebula fruit extracts displayed potent growth inhibitory activity in the disc diffusion assay against Y. enterocolitica. The methanolic and ethyl acetate T. chebula fruit extracts were particularly potent growth inhibitors, with MIC values of 85 and 64 µg/mL respectively. The aqueous fruit extract also displayed good growth inhibitory activity against Y. enterocolitica, albeit with a higher MIC value (653 µg/mL). The T. arjuna branch extract was moderately active (3000 µg/mL). All other extracts were either low efficacy, or completely devoid of growth inhibitory activity. All Indian Terminalia spp. extracts were nontoxic (LC 50 values <1000 µg/mL) in the Artemia franciscana bioassay. Conclusions: The lack of toxicity and the potent growth inhibitory bioactivity of the T. chebula extracts against Y. enterocolitica indicates their potential as medicinal agents in the treatment and prevention of yersiniosis.

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Prevalence of Pathogenic Yersinia enterocolitica Strains in Pigs in the United States

Cited by 95 publications

References 15 publications

The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes

The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes

Yersinia Enterocolitica: An Unlikely Cause of Positive Brucellosis Tests in Greater Yellowstone Ecosystem Bison (Bison Bison)

Investigating the Pharmacognostic Potential of Indian Terminalia Spp. in the Treatment and Prevention of Yersiniosis

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