Prevalence of plasmid-mediated quinolone resistance determinants qnr and aac(6′)-Ib-cr in Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases in Spain

Briales, Alejandra; Rodríguez-Martínez, José Manuel; Velasco, C.; Alba, Paula Díaz de; Rodríguez-Baño, Jesús; Martínez-Martínez, Luis; Pascual, Álvaro

doi:10.1016/j.ijantimicag.2011.12.009

Cited by 105 publications

(69 citation statements)

References 13 publications

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“…The rates were relatively high, namely 56% of qnrB-positive isolates of Klebsiella pneumoniae which were also ESBL-positive. This is in contrast with European studies showing generally lower percentages of qnr genes in clinical isolates of ESBL-positive strains (4% in Spain or France and 9% in Norway or Sweden), but higher levels of the aac(6`)-1b-cr gene (16% in Spain, 23% in France and 52% in Norway or Sweden) (20)(21)(22).…”

Section: Discussioncontrasting

confidence: 84%

“…The presence of PMQR genes in both animals and humans is suggestive of potential horizontal transfer of the resistance genes between strains (18,(20)(21)(22).…”

Section: Discussionmentioning

confidence: 99%

“…While qnr genes do not exceed 10%, the prevalence of the aac(6`)-1b-cr gene ranges from 16% to 52%. Generally, higher rates of the two genes are observed in ESBL-positive strains (20)(21)(22).…”

Section: Introductionmentioning

confidence: 97%

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Quinolone-resistant Escherichia coli in Poultry Farming

Hricová¹,

Röderová²,

Pudová³

et al. 2017

Cent Eur J Public Health

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SUMMARYIncreasing bacterial resistance to quinolone antibiotics is apparent in both humans and animals. For humans, a potential source of resistant bacteria may be animals or their products entering the human food chain, for example poultry. Between July 2013 and September 2014, samples were collected and analyzed in the Moravian regions of the Czech Republic to isolate the bacterium Escherichia coli. As a result, 212 E. coli isolates were obtained comprising 126 environmental isolates from poultry houses and 86 isolates from cloacal swabs from market-weight turkeys. Subsequently, the E. coli isolates were tested for susceptibility to selected antibiotics. Resistance of the poultry isolates to quinolones ranged from 53% to 73%. Additionally, the presence of plasmid-mediated resistance genes was studied. The genes were confirmed in 58% of the tested strains. The data on resistance of isolates from poultry were compared with results of resistance tests in human isolates obtained in the same regions.The high levels of resistance determined by both phenotyping and genotyping methods and reported in the present study confirm the fact that the use of fluoroquinolones in poultry should be closely monitored.

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Section: Discussioncontrasting

confidence: 84%

“…The presence of PMQR genes in both animals and humans is suggestive of potential horizontal transfer of the resistance genes between strains (18,(20)(21)(22).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Quinolone-resistant Escherichia coli in Poultry Farming

Hricová¹,

Röderová²,

Pudová³

et al. 2017

Cent Eur J Public Health

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“…The existence of resistance to quinolone in ESBL-producing isolates has been previously reported (29). In the present study, percentage of quinolone resistant isolates (68%) was substantially higher than those in Spain (19.4%) (30), Italy (44%) (31), and France (30%) (32). Warburg et al showed that resistance to fluoroquinolone during a periods of 14 years in nosocomial isolates of E. coli has increased from 11.3% to 46.6%, and also reported that from 1991 to 1997, no isolate had aac(6')-Ib-cr, whereas from 1998 onwards, 7.1% of the isolates had aac(6')-Ib-cr (32).…”

Section: Discussionsupporting

confidence: 48%

“…According to this study, in Spain, the aac(6')-Ib-cr gene was the most prevalent PMQR gene in E. coli and K. pneumoniae-producing ESBL (30).…”

Section: Discussionmentioning

confidence: 79%

Quinolone Resistance Determinants qnr, qep, and aac(6’)-Ib-cr in Extended-Spectrum Β-Lactamase Producing Escherichia coli Isolated From Urinary Tract Infections in Tehran, Iran

Goudarzi

Fazeli

2017

Shiraz E-Med J

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Background: Prevalence of plasmid mediated quinolone resistance in Escherichia coli clinical isolates is a serious problem in developing countries. Objectives:The aim of this study was to investigate quinolone resistance determinants among Extended-Spectrum B-Lactamases (ESBL)-producing E. coli recovered from patients with nosocomial urinary tract infection. Methods: A total of 290 E. coli isolates, obtained from patients with UTI, were included in this study. The phenotypic confirmatory test for ESBL production was performed by double disc synergy test and combined disk diffusion test. Kirby-Bauer Disk diffusion method was performed to test susceptibilities of ESBL-producing E. coli isolates to 15 antimicrobial agents. Isolates were screened for the presence of ESBL and plasmid-mediated quinolone resistance genes by polymerase chain reaction (PCR). Results: In the present study, 290 (71.7%) E. coli strains were recovered from 410 hospitalized patients with UTI, 51.7% of which were found to have ESBL positive results. In vitro, the susceptibilities of ESBL-producing E. coli strains showed that all isolates were resistant to amoxicillin and penicillin and resistance rates to other of antibiotics differed from 40% to 96%. Among the 150 ESBL positive isolates, frequencies of aac(6')- Ib, oqxA, oqxB, qnrA, qnrB, qnrS, and qepA were 74.7%, 8%, 4%, 3.3%, 1.3%, 2%, and 2.7%, respectively. Coexistence of bla CTX-M, bla TEM, bla CTX-M, and aac(6')-Ib were the most widely distributed resistance genotypes. Conclusions:The data of the present study revealed the high prevalence of plasmid-mediated quinolone resistance genes among ESBL-producing E. coli in the hospitals. The bla CTX-M genes were found to be the dominant ESBL-encoding gene.

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Defining the Relationship Between Phenotypic and Genotypic Resistance Profiles of Multidrug-Resistant Enterobacterial Clinical Isolates

Galal

Aziz

Hassan

2018

Advances in Experimental Medicine and Biology

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We suggest using blaTEM, blaCTX-M-15, qnr, and aac(6')-Ib-cr genes for better and faster prediction of suitable antibiotic therapy with effective doses against ESBL-producing isolates harboring plasmid-mediated quinolone resistance (PMQR) determinants. Amikacin, meropenem, gentamicin, and imipenem seem to be better choices of treatment for such life-threatening infections, because of their remaining highest activity.

show abstract

Prevalence of plasmid-mediated quinolone resistance determinants qnr and aac(6′)-Ib-cr in Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases in Spain

Cited by 105 publications

References 13 publications

Quinolone-resistant Escherichia coli in Poultry Farming

Quinolone-resistant Escherichia coli in Poultry Farming

Quinolone Resistance Determinants qnr, qep, and aac(6’)-Ib-cr in Extended-Spectrum Β-Lactamase Producing Escherichia coli Isolated From Urinary Tract Infections in Tehran, Iran

Defining the Relationship Between Phenotypic and Genotypic Resistance Profiles of Multidrug-Resistant Enterobacterial Clinical Isolates

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