Prevalence of plasmid-mediated quinolone resistance determinants among oxyiminocephalosporin-resistant Enterobacteriaceae in Argentina

Cruz, Giovanna Rincón; Radice, Marcela; Sennati, Samantha; Pallecchi, Lucia; Rossolini, Gian María; Gutkind, Gabriel; Conza, José Alejandro Di

doi:10.1590/0074-0276130084

Cited by 23 publications

(14 citation statements)

References 18 publications

(21 reference statements)

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“…overall PMQR prevalence found here was similar to that observed in phenotypically unselected clinical enterobacteria from Spain (6.4%) 22 and was well below those found in the two earlier surveys from Argentina, namely 57% 23 and 60%, 24 because both studies were based on selected isolates with resistance/decreased susceptibility to quinolones and ESC resistance, respectively.…”

Section: Occurrence and Prevalence Of Pmqr Genessupporting

confidence: 84%

“…21,22 In regard to Argentina, only two surveys were reported and they addressed PMQR prevalence in clinical enterobacteria with resistance/decreased susceptibility to quinolones 23 or ESC resistance. 24 Here, we report a large nationwide study based on a point prevalence collection of 1058 unselected clinical enterobacteria that we conducted to estimate the prevalence of all the PMQR genes currently known.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of Plasmid-Mediated Quinolone Resistance Genes in Clinical Enterobacteria from Argentina

Albornoz

Lucero

Romero

et al. 2017

Microbial Drug Resistance

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This first nationwide study was conducted to analyze the prevalence of plasmid-mediated quinolone resistance (PMQR) genes in phenotypically unselected (consecutive) clinical enterobacteria. We studied 1,058 isolates that had been consecutively collected in 66 hospitals of the WHONET-Argentina Resistance Surveillance Network. Overall, 26% of isolates were nonsusceptible to at least one of the three quinolones tested (nalidixic acid, ciprofloxacin, and levofloxacin). The overall prevalence of PMQR genes was 8.1% (4.6% for aac(6')-Ib-cr; 3.9% for qnr genes; and 0.4% for oqxA and oqxB, which were not previously reported in enterobacteria other than Klebsiella spp. from Argentina). The PMQR prevalence was highly variable among the enterobacterial species or when the different genes were considered. The prevalent PMQR genes were located in class 1 integrons [qnrB2, qnrB10, and aac(6')-Ib-cr]; in the ColE1-type plasmid pPAB19-1 or Tn2012-like transposons (qnrB19); and in Tn6238 or bracketed by IS26 and bla [aac(6')-Ib-cr]. The mutations associated with quinolone resistance that were located in the quinolone resistance-determining region (QRDR mutations) of gyrA, parC, and gyrB were also investigated. The occurrence of QRDR mutations was significantly associated with the presence of PMQR genes: At least one QRDR mutation was present in 82% of the PMQR-harboring isolates but in only 23% of those without PMQR genes (p < 0.0001, Fisher's Test). To the best of our knowledge, this is the first report on the prevalence of PMQR genes in consecutive clinical enterobacteria where all the genes currently known have been screened.

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Section: Occurrence and Prevalence Of Pmqr Genessupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Prevalence of Plasmid-Mediated Quinolone Resistance Genes in Clinical Enterobacteria from Argentina

Albornoz

Lucero

Romero

et al. 2017

Microbial Drug Resistance

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“…In Argentina, qnrB1, qnrB2, qnrB4, qnrB6, qnrB10, qnrB19, and qnrS have been found. [36][37][38] In Bolivia, qnrS and qnrB; 39,40 in Peru, qnrB19 and qnrS; 40,41 in Uruguay, qnrA1, qnrB1, qnrB4, qnrB8-like, qnrB13, and qnrB17; 14,42 and in Venezuela qnrB19, 43 have been reported. The qnr genes have been described in a variety of microorganisms isolated either from colonization, or from nosocomial and community-acquired infections.…”

Section: Discussionmentioning

confidence: 99%

Sepsis caused by New Delhi metallo-β-lactamase (blaNDM-1) and qnrD-producing Morganella morganii, treated successfully with fosfomycin and meropenem: case report and literature review

Seija

Presentado

Bado

et al. 2015

International Journal of Infectious Diseases

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“…Elsewhere in the world, the qnr B6 gene has been detected in South Korea in a strain of Enterobacter aerogenes producing broad spectrum β-lactamases from a collection of 644 enterobacteriaceae from 12 clinical laboratories [20]. Similarly, in Argentina, Cruz et al [21] reported the presence of qnr B6 in 5% of enterobacteriaceae that produce broad spectrum β-lactamase. Also, in Morocco, the qnr B6 gene was detected in 0.9% of strains producing extended spectrum β-lactamasess tested in the work of Jamali et al [22].…”

Section: Discussionmentioning

confidence: 99%

First Case of Qnr B6 and Qnr B7 Genes in Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Abidjan, Côte D'ivoire

Victoire

Anatole

Fernique³

et al. 2019

SAJRM

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Aims: The aim of this study was to characterize fluoroquinolone resistance genes in enterobacteriaceae that produce extended-spectrum β-lactamases, isolated in Abidjan. Place and Duration of Study: Pasteur Institute of Côte d'Ivoire and research unit on emerging tropical infectious diseases of Aix-Marseille University from January 2017 at July 2017. Methodology: The study included 90 enterobacteriaceae producing extended-spectrum β-lactamases isolated from biological products from various hospital services in Abidjan. These strains have been pre-identified and stored at the Center for Biological Resources (CeReB) of Pasteur Institute of Côte d'Ivoire. The identification of the strains was confirmed using the mass spectrometry MALDI-TOF (MS) and the antibiotic sensitivity test was performed using Müeller Hinton's agar diffusion method. The fluoroquinolone resistant genes were detected by conventional PCR and then, sequenced. Results: The strains studied were Escherichia coli (44), Klebsiella pneumoniae (31) and Enterobacter cloacae (15). High resistance rates to ceftriaxone (96.7%), cefotaxime (95.6%), aztreonam (95.6%) and cefoxitin (72.2%) were observed in all strains producing broad spectrum β-lactamases. The resistance rate to fluororquinolones represented by ciprofloxacin was 86.7%. The fluoroquinolone resistance genes detected were qnr A (3.3%) and qnr B (42.2%). Sequencing identified the qnr A1 (3.3%), qnr B1 (31.1%), qnr B6 (2.2%) and qnr B7 (1.1%) genes. Conclusion: This study made it possible to identify fluoroquinolone resistance genes in enterobacteriaceae producing β-lactamases which have an extended spectrum in Abidjan.

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Prevalence of plasmid-mediated quinolone resistance determinants among oxyiminocephalosporin-resistant Enterobacteriaceae in Argentina

Cited by 23 publications

References 18 publications

Prevalence of Plasmid-Mediated Quinolone Resistance Genes in Clinical Enterobacteria from Argentina

Prevalence of Plasmid-Mediated Quinolone Resistance Genes in Clinical Enterobacteria from Argentina

Sepsis caused by New Delhi metallo-β-lactamase (blaNDM-1) and qnrD-producing Morganella morganii, treated successfully with fosfomycin and meropenem: case report and literature review

First Case of Qnr B6 and Qnr B7 Genes in Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Abidjan, Côte D'ivoire

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