Prevalence of porA pseudogene deletion among Neisseria gonorrhoeae isolates referred to the UK’s Gonococcal Resistance to Antimicrobials Surveillance Program

Toby, Martina; Saunders, Pamela; Cole, Michelle; Grigorjev, Vlad; Alexander, Sarah; Ison, Catherine

doi:10.1071/sh16162

Cited by 6 publications

(5 citation statements)

References 7 publications

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“…Like any molecular target, mutations of the porA gene could result in false-negative results test results. However, recent antimicrobial resistance studies have shown that porA mutants represented less than 0.4% of clinical NG isolates (Toby et al 2017). Primer sequences and assay components, originally designed by Biohelix Inc. (Quidel Corporation, San Diego, CA), are listed in Table S1.…”

Section: Methodsmentioning

confidence: 99%

A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples

Horst

Rosenbohm

Kolluri

et al. 2018

Biomed Microdevices

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Globally, the microbe Neisseria gonorrhoeae (NG) causes 106 million newly documented sexually transmitted infections each year. Once appropriately diagnosed, NG infections can be readily treated with antibiotics, but high-risk patients often do not return to the clinic for treatment if results are not provided at the point of care. A rapid, sensitive molecular diagnostic would help increase NG treatment and reduce the prevalence of this sexually transmitted disease. Here, we report on the design and development of a rapid, highly sensitive, paperfluidic device for point-of-care diagnosis of NG. The device integrates patient swab sample lysis, nucleic acid extraction, thermophilic helicase-dependent amplification (tHDA), an internal amplification control (NGIC), and visual lateral flow detection within an 80 min run time. Limits of NG detection for the NG/NGIC multiplex tHDA assay were determined within the device, and clinical performance was validated retroactively against qPCR-quantified patient samples in a proof-of-concept study. This paperfluidic diagnostic has a clinically relevant limit of detection of 500 NG cells per device with analytical sensitivity down to 10 NG cells per device. In triplicate testing of 40 total urethral and vaginal swab samples, the device had 95% overall sensitivity and 100% specificity, approaching current laboratory-based molecular NG diagnostics. This diagnostic platform could increase access to accurate NG diagnoses to those most in need.

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Section: Methodsmentioning

confidence: 99%

A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples

Horst

Rosenbohm

Kolluri

et al. 2018

Biomed Microdevices

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“…All archived isolates were retrieved from -80C and inoculated on to non-selective GCVIT agar (GC agar base (Becton, Dickinson and Co, Le Pont de Claix, France) containing 1% Vitox (Oxoid, Basingstoke, UK)). Inoculated plates were incubated at 36C in 5% CO2 for 18-24 h. Growth was subcultured on to GCVIT agar plates and incubated again at 36C in 5% CO2 for 18-24 h. Identification of isolates as N. gonorrhoeae had previously been performed by real-time PCR using opa and porA targets, 10 or by MALDI-ToF.…”

Section: N Gonorrhoeae Isolatesmentioning

confidence: 99%

What’s left in the cupboard? Older antimicrobials for treating gonorrhoea

Fifer

Livermore

Uthayakumaran

et al. 2021

Journal of Antimicrobial Chemotherapy

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Background Neisseria gonorrhoeae has developed resistance to all antimicrobials used to treat gonorrhoea, with even ceftriaxone being undermined. It is therefore important to examine any potential to redeploy older antimicrobials routinely used for other infections to treat ceftriaxone-resistant gonococcal infections. Objectives We examined the susceptibility of N. gonorrhoeae to aztreonam, chloramphenicol, co-trimoxazole, fosfomycin, piperacillin/tazobactam and rifampicin. Methods N. gonorrhoeae isolates (n = 94) were selected to include a range of antimicrobial susceptibilities: 58 were collected in the Gonococcal Resistance to Antimicrobials Surveillance Programme; 17 were clinical isolates referred to the PHE reference laboratory; and 19 were control strains. MICs were determined by agar dilution for the six study antimicrobials and for ceftriaxone and azithromycin as comparators. Results There was correlation between piperacillin/tazobactam and ceftriaxone MICs, but all five isolates with high ceftriaxone MICs (>0.5 mg/L) were inhibited by piperacillin/tazobactam at 0.06–0.5 mg/L. Aztreonam MICs for ceftriaxone-resistant isolates exceeded those of ceftriaxone. Among non-β-lactams, fosfomycin and co-trimoxazole had low, tightly clustered MICs, suggesting widespread susceptibility, rifampicin split the collection into highly susceptible and highly resistant groups and chloramphenicol had a wide MIC distribution. Conclusions Although unsuitable for empirical use, piperacillin/tazobactam, fosfomycin, co-trimoxazole, rifampicin and, possibly, chloramphenicol could be considered for individual patients with ceftriaxone-resistant gonococcal infection once MICs are known. Wider surveillance of the susceptibility of N. gonorrhoeae to these agents is needed, along with clinical trials and the establishment of clinical breakpoints for N gonorrhoeae.

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“…A possible justification for these discrepancies may be attributable to geographic and www.nature.com/scientificreports/ climatic variations in different regions of world. Some of the studies documented a very low prevalence of NG in cases of European population 36 while other studies reported a very high prevalence in American population 37 .…”

Section: Discussionmentioning

confidence: 99%

Functional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian women

Sharma

Sonkar

Singhal

et al. 2021

Sci Rep

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The aim of the present study is to investigate the functional role of TNF-α single-nucleotide polymorphisms/haplotypes in an association with reproductive tract infections (RTIs) in symptomatic and asymptomatic women. A total of 850 consecutive subjects consisting of 400 cases and 450 healthy controls, were screened for RTIs, along with their risk factors and associated symptoms. The propensity score matching was performed to reduce the confounding bias arise owing to covariates and to balance the data between two groups. A total of 211 pairs (1:1) have been created. Genotyping of rs1800629 (-308) and rs361525 (-238) SNPs of TNF-α was done by PCR–RFLP followed by sequencing. The functional implication of TNF-α SNPs in an association with RTIs was also checked by using ELISA. The frequency of -238A allele and -308A allele was found to be twofold (P < 0.0001) and threefold (P < 0.0001) higher in the presence of RTIs. AA haplotype emerged as a major player in an association with RTIs and elevated TNF-α expression. The present study revealed the functional role of rs1800629 (-308) and rs361525 (-238) of TNF-α in an association with RTIs. This information may be used to establish biomarkers for an inflammatory response during the persistence of RTIs.

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Prevalence of porA pseudogene deletion among Neisseria gonorrhoeae isolates referred to the UK’s Gonococcal Resistance to Antimicrobials Surveillance Program

Cited by 6 publications

References 7 publications

A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples

A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples

What’s left in the cupboard? Older antimicrobials for treating gonorrhoea

Functional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian women

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