Prevalence of Salmonella Spp. Bacteria Antibiotic Resistency Indigestion Tract in the Broiler Farms of Subang District

Abstract

This study is aimed to estimate the prevalence of resistant Salmonella spp., determine the spread of bacterial resistance and investigate the serotypes of bacteria in the chicken’s digestion tract in the broiler farms in Subang District. As many as 74 farms were chosen, five poled caeca samples were taken from each farm and tested for isolation and identification of Salmonella spp. Salmonella isolates obtained were tested antimicrobial susceptibility against 8 antibiotics using the agar dilution method. The antibiotics were gentamycin, tetracycline, ciprofloxacin, nalidixic acid, ampicillin, chloramphenicol, trimethoprim, and sulfamethoxazole. The result showed that 8 out of 74 samples were positive for Salmonella. The prevalence of Salmonella spp. in the digestion tract was 10.8%; 95% confidence interval 3.7%-17.9%. Based on the serological test eight serotypes obtained were Salmonella enteritidis, Salmonella oslo, Salmonella narashino, Salmonella nakuru, and Salmonella nordufer. The result of antibiotic resistance test showed that from 8 Salmonella isolates obtained, 12.5% were found to be sensitive, 75% isolates were resistant to one or two antibiotics, and the remaining 12.5% isolates were resistant to more than two antibiotics; 95% confidence interval (0%-35.4%). The prevalence of resistant Salmonella spp. bacteria in chicken digestion tract in broiler farms in Subang District was high, so integrated control program to reduce antimicrobial resistance problem in broiler farm are greatly needed.

Detection Of Antibiotic Residues in Eggs of Layer Chickens and Knowledge of Animal Officers About Antibiotics

“…It has been reported that most livestock workers in Subang district, as many as 59% of respondents had common knowledge of antibiotic resistance. As many as 41% of farmer respondents, had a high level of knowledge (Walyani, 2019). The results of a survey on farmer perceptions of antibiotics on farms in West Kalimantan were conducted on 293 commercial broiler breeders.…”

“…The use of antibiotics in feed is closely related to the incidence of resistance to three strains of pathogenic bacteria such as Salmonella, Campylobacter, and Escherichia coli. These bacteria are three of the five main causes of foodborne disease or the cause of death due to foodborne pathogen infection in the United States which are around 90% (Walyani, 2019). Kusumawardani (2016) reported the results of sensitivity tests to chloramphenicol antibiotic in which 2 from 17 strains of E. coli showed resistance.…”

Microbial Count and <i>AvBD10</i> Expressions in Ovaries and Oviducts of Kampung Unggul Balitbangtan (KUB)-1 Chickens Following Intravaginally CpG-ODN and <i>S</i>. Enteritidis

“…Gong et al (2022) reported that in a global context, non-typhoidal Salmonella (NTS) leads to 93.8 million cases of gastroenteritis each year, resulting in 155,000 deaths. Investigation in Indonesia found high Salmonella contamination rates in chicken meat (85%), intestine contents (57.5%), and rinse water (52.5%) at traditional markets in Surabaya as reported by Yulistiani et al (2019), meanwhile Walyani et al (2019) reported in Subang, 75% isolates of Salmonella spp. which infects chickens were resistant to antibiotics with a 95% confidence interval (0%-35.4%), one of which was SE.…”