Prevalence of sickle cell disorders and malaria infection in children aged 1–12 years in the Volta Region, Ghana: a community-based study

Oppong, Mavis; Lamptey, Helena; Kyei-Baafour, Eric; Aculley, Belinda; Ofori, Ebenezer Addo; Tornyigah, Bernard; Kweku, Margaret; Ofori, Michael F.

doi:10.1186/s12936-020-03500-5

Cited by 13 publications

(10 citation statements)

References 42 publications

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“…falciparum infection did not differ among the three sites, but the median parasite density was significantly higher at Krachi than the two other study sites. Parasite prevalence by PCR (already described in [ 23 ]) was higher than prevalence by microscopy. It did not however differ between the three sites ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…The study involved children aged 1–12 years living in communities within the selected districts. The study site and population have been described elsewhere [ 23 ]. Briefly, the study employed a multi-stage sampling method in which the three [ 3 ] ecological zones of the Volta Region, southern, middle and northern zones reflecting the three malaria transmission zones, were used.…”

Section: Methodsmentioning

confidence: 99%

“…Parasite density was estimated against 500 leucocytes, using an assumed leucocyte count of 8,000/μL of blood and expressed as parasites/μL. Polymerase Chain Reaction (PCR) data to measure sub-microscopic parasitaemia from the same cohort has already been published elsewhere [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

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Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern

et al. 2021

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Detection of antibody reactivity to appropriate, specific parasite antigens may constitute a sensitive and cost-effective alternative to current tools to monitor malaria transmission across different endemicity settings. This study aimed to determine the suitability of IgG responses to a number of P. falciparum antigens as markers of transmission intensity and pattern. Antibody responses to multiple malaria antigens were determined in 905 participants aged 1–12 years from three districts with low (Keta), medium (Hohoe) and high (Krachi) transmission intensity in the Volta region of Ghana. Blood film microscopy slides and dry blood spots (DBS) were obtained for parasitaemia detection and antibody measurement, respectively. Sera were eluted from DBS and levels of IgG specific for 10 malaria antigens determined by a multiplex assay. Results were compared within and among the districts. Total IgG responses to MSPDBL1, MSPDBLLeucine, MSP2-FC27, RAMA, and PfRh2a and PfRh2b were higher in Krachi than in Hohoe and Keta. Seroprevalence of IgG specific for MSPDBLLeucine, RON4, and PfRh2b were also highest in Krachi. Responses to RALP-1, PfRh2a and PfRh2b were associated with patent but asymptomatic parasitaemia in Keta, while responses to MSPDBL1, MSPDBLLeucine, MSP2-FC27, RAMA, Rh2-2030, and PfRh2b were associated with parasite carriage in Hohoe, but not in Krachi. Using ROC analysis, only PfRh2b was found to predict patent, but asymptomatic, parasitaemia in Keta and Hohoe. Antibody breadth correlated positively with age (r = 0.29, p<0.0001) and parasitaemia (β = 3.91; CI = 1.53 to 6.29), and medium to high transmission (p<0.0001). Our findings suggest differences in malaria-specific antibody responses across the three transmission zones and that PfRh2b has potential as a marker of malaria transmission intensity and pattern. This could have implications for malaria control programs and vaccine trials.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern

et al. 2021

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“…All children sampled had no clinical symptoms. The population and sites of the larger study have been described in detail elsewhere (Oppong et al, 2020;Kyei-Baafour et al, 2021).…”

Section: Population and Study Sitesmentioning

confidence: 99%

Serology reveals micro-differences in Plasmodium falciparum transmission in the Hohoe municipality of Ghana

Kyei-Baafour

Kusi²,

Oppong³

et al. 2023

Front. Parasitol.

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BackgroundWith the decline in malaria transmission due to global efforts, a more sensitive tool is needed to monitor transmission intensity and pattern at the micro-level. Though transmission in a broader area may be similar, factors such as sanitation, practices of open water storage, early morning and evening activities, outdoor sleeping and agricultural practices within communities could cause differences in exposure and thus transmission. This study thus probed malaria transmission at a micro-level using serology in the Hohoe Municipality of Ghana.MethodsThis cross-sectional study involved 327 asymptomatic children aged 1-12 years in both rural (196) and urban (131) communities in the Hohoe municipality. Total IgG responses specific for three P. falciparum antigens (CSP, MSP2-FC27, MSP2-3D7) were determined in plasma eluted from dried blood spots using indirect ELISA.ResultsA higher proportion of individuals in the rural area had parasites by both microscopy and PCR. Total IgG levels and seroprevalence were higher in rural compared to urban communities (p<0.05). In a multiple regression model, adjusting for confounders, levels of PfMSP2-3D7-specific IgG was associated with the higher transmission which occurs in the rural community.ConclusionThe results suggest that though the district is categorized as having medium malaria transmission, differences within settlements may influence malaria transmission reflecting in antibody levels and prevalence of malaria antigen-specific IgG.

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“…In the tropics, SCD is commonly present in the severe homozygous SS (HbSS), heterozygous SC (HbSC), and the beta-zero or beta-plus thalassemia (HbSβ°/ HbSβ + thalassemia). 1,2 Globally, it is estimated that nearly 5% of the world's population have SCT, and almost 300,000 babies are born with SCD annually. 3,4 Ghana significantly contributes to this global prevalence, with about 2% (approximately 15,000) of all newborns having SCD, and more than half have the severe form of the disease.…”

Section: Introductionmentioning

confidence: 99%

Assessing knowledge of sickle cell disease and health beliefs on premarital genetic screening among healthcare trainees at a tertiary institution: A cross‐sectional study

Agbozo

Amanor

Acheampong

et al. 2023

Health Science Reports

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Background The uptake of sickle cell trait (SCT) test is challenged by several factors. A community of healthcare professionals educating the public to undergo screening is critical in reducing the disease burden. We investigated knowledge and attitude towards premarital SCT screening among healthcare trainee students who are the next generation of healthcare practitioners. Methods A cross‐sectional design was employed, and quantitative data were collected from 451 female students pursuing healthcare programs at a tertiary institution in Ghana. Descriptive, bivariate, and multivariate logistic regression analysis was performed. Results More than half of the participants were 20–24 years (54.55%) and had good knowledge (71.18%) about sickle cell disease (SCD). Age and school or social media as sources of information were significantly associated with good knowledge about SCD. Students between the age 20–24 (adjusted odds ratio [AOR] = 2.54, confidence interval [CI] = 1.30–4.97) and knowledge (AOR = 2.19, CI = 1.41–3.39) were 3 times and 2 times more likely to have a positive perception about SCD severity. Students who have SCT (AOR = 5.16, CI = 2.46–10.82), whose source of information was family member/friends (AOR = 2.83, CI = 1.44–5.59) and social media (AOR = 4.59, CI = 2.09–10.12) were 5 times, 2 times and 5 times likely to have a positive perception about the susceptibility of SCD. Students whose source of information is school (AOR = 2.06, CI = 1.11–3.81) and who have good knowledge of SCD (AOR = 2.25, CI = 1.44–3.52) were 2 times more likely to have a positive perception about the benefits of testing. Students with SCT (AOR = 2.64, CI = 1.36–5.13) and source of information was social media (AOR = 3.01, CI = 1.36–6.64) were about 3 times more likely to have a positive perception about the barriers to testing. Conclusion Our data shows that high level of SCD knowledge influences positive perceptions about the severity of SCD, the benefits and relatively low barriers to SCT or SCD testing and genetic counseling. Dissemination of SCT, SCD and premarital genetic counseling education should be intensified especially in schools.

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Prevalence of sickle cell disorders and malaria infection in children aged 1–12 years in the Volta Region, Ghana: a community-based study

Cited by 13 publications

References 42 publications

Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern

Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern

Serology reveals micro-differences in Plasmodium falciparum transmission in the Hohoe municipality of Ghana

Assessing knowledge of sickle cell disease and health beliefs on premarital genetic screening among healthcare trainees at a tertiary institution: A cross‐sectional study

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