Prevalence of ST1193 clone and IncI1/ST16 plasmid in E-coli isolates carrying blaCTX-M-55 gene from urinary tract infections patients in China

Abstract

To study molecular epidemiology of CTX-M-55-carrying Escherichia coli isolates from urinary tract infections (UTIs) in China. 111 blaCTX-M-55-positive E.coli isolates from UTIs patients in China were studied. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homologies among the strains. Conjugation experiments, S1nuclease PFGE and PCR analysis were performed to characterize plasmids harboring blaCTX-M-55 and their genetic environment. 111 isolates were clustered into 86 individual pulsotypes and three clusters by PFGE. Fifty-five (49.5%) of the isolates belonged to 8 STs. Most of the ST1193 isolates belonged to one PFGE cluster. Transconjugants (n = 45) derived from randomly selected blaCTX-M-55 donors (n = 58), were found to contain a single 90-kb conjugative plasmid, which mainly belonged to the IncI1 groups (34, 76%). Among the IncI1 plasmids, the blaCTX-M-55/IncI1/ST16 predominated (23/34, 68%). The blaTEM-1 and aac (3′)-II genes were frequently detected on the IncI1 plasmids, and the insertion of ISEcp1 or IS26 was observed at the 48 bp or 45 bp upstream of the start codon of blaCTX-M-55 gene. The dissemination of blaCTX-M-55 gene among E. coli UTI isolates, appeared to be due to both the major clonal lineage of ST1193 and the horizontal transfer of epidemic plasmid IncI1/ST16.

Antimicrobial resistant and extended‐spectrum β‐lactamase producing Escherichia coli in common wild bird species in Switzerland

“…This clone has very recently emerged as an MDR bla CTX‐M‐14 and bla CTX‐M‐15 harboring nosocomial pathogen in Germany (Valenza et al., ). Further, E. coli ST1193 harboring bla CTX‐M‐55 represents one of the most prevalent ESBL‐producing E. coli associated with UTI in China (Xia et al., ). Our results indicate that wild birds may contribute to the global dissemination of this important clonal lineage.…”

Rapid Emergence, Subsidence, and Molecular Detection of Escherichia coli Sequence Type 1193- fimH64 , a New Disseminated Multidrug-Resistant Commensal and Extraintestinal Pathogen

“…The emerging resistance is due mainly to a few widely disseminated clones, most notably the H30R subclone of sequence type 131 (ST131-H30R; associated with allele 30 of fimH [type 1 fimbrial adhesin]), which derives from virulence-associated phylogroup B2 (3)(4)(5)(6). Recent reports from diverse locales describe a possible new such clone, ST1193, which likewise is from phylogroup B2 but, unlike ST131, represents sequence type complex 14 (STc14) and is associated with fimH allele 64, i.e., ST1193-H64 (7)(8)(9)(10)(11)(12).…”

“…Subsequent reports from China (9,10), Korea (11), Norway (12), Germany (13), and the United States (8) documented ST1193 as a progressively emerging FQ-R human pathogen in these regions, often second only to ST131-H30R among FQ-R isolates and sometimes associated with CTX-M extended-spectrum beta-lactamases (ESBLs) (9,12). These isolates' virulence genotype, O type, and lactose-negative phenotype, when reported, have resembled those of the 2008 Australian isolates, suggesting global clonal dissemination.…”

Rapid and Extensive Expansion in the United States of a New Multidrug-resistantEscherichia coliClonal Group, Sequence Type 1193

“…occurrence among FQ-R E. coli isolates of the clonal group ST1193 [2][3][4][5][6]. Here, we analyze whether, besides H30, any other clonal expansion is evident among FQ-R E. coli isolates collected in the course of a multicenter surveillance study.…”