Prevalence of subclinical mastitis and isolated udder pathogens in dairy cows in Southern Vietnam

Östensson, K.; Lam, Vo; Sjögren, Natahlie; Wredle, Ewa

doi:10.1007/s11250-012-0320-0

Cited by 31 publications

(28 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This confirms that the prevalence of coliform bacteria in this study would be low, as few samples were of clinical mastitis. Although environmental Streptococcus agalactiae (6.5%) were ranked third in the current study, the microorganism have been reported as the most prominent bacteria in cow mastitis in some countries [26,45]. The prevalence rate of S. agalactiae reported in the current study agrees with previous findings observed elsewhere [35,59].…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Prevalence and Economic Impacts of Retained Placenta and Subclinical Mastitis in Tanzanian Dairy Cows

Komba¹,

Kashoma²

2020

Preprint

View full text Add to dashboard Cite

Background: Three dairy farms were investigated for occurrence of retained placenta and subclinical mastitis, and the economic impacts attributable to the conditions. Reproductive performance data from 1169 calvings of dairy cows for five consecutive years spanning from 2014 to 2018 were used in this study. A total of 167 cows were screened with the California Mastitis Test (CMT), and udder quarters with CMT score of ≥ 3 (scale 1–5) were milk sampled for culture and final bacteriological identification. Results: Overall prevalences of subclinical mastitis and retained fetal membranes were 35.93% and 10.35%, respectively. The prevalence of bacterial isolates at animal level was 38.92%. Individual bacteria prevalence rates were 66.15% for Staphylococcus aureus, 13.85% for Escherichia coli, 6.15% for Bacillus spp., 6.15% for Streptococcus agalactiae and 7.69% for none typed microorganisms. Overall loss from subclinical mastitis was estimated at 10,603,800/= Tanzanian shillings (Tshs) equivalent to USD $ 4,700 with major losses (81.75%) attributable to the production loss followed by treatment expenditure (18.25%). Average loss attributed to mastitis per quarter was estimated at 49,320/= Tshs (USD $21.5). Retained placenta resulted in an increase (P<0.05) in the period from parturition to conception (148.7 vs. 96.0 days), number of services per conception (2.9 vs. 1.9) and calving interval (434.7 vs. 374.0 days). Conclusion: These findings point out to the economic importance of subclinical mastitis and retained fetal membranes in the dairy industry. This calls for improved milking hygiene, proper attention to health of mammary glands, regular screening tests and application of biosecurity measures as intervention strategies to lower the prevalence of subclinical mastitis and minimize the spread of pathogens in dairy farms. Since retained fetal membrane is an important problem causing great economic losses, appropriate control measures have to be instituted including cow comfort, proper immunization and careful nutritional management (mineral supplementation), particularly during the transition period.

show abstract

Section: Discussionsupporting

confidence: 90%

“…Vietnam [45]. It has been pointed out by previous authors that subclinical mastitis is a complex disease and discrepancies in the reported incidences in different studies could be attributed to differences in animal breeds, management systems, parity, teat morphology, and milking hygiene [46,47].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Economic Impacts of Retained Placenta and Subclinical Mastitis in Tanzanian Dairy Cows

Komba¹,

Kashoma²

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Studies on the prevalence rate of clinical and subclinical mastitis of different mastitis pathogens in a cow population from small-scale dairy herds are scarce. Although it is difficult to compare results obtained in this work with those obtained in other countries, CNS, S. aureus , and streptococci have been reported to be the most prevalent pathogens [52, 53]. Alternatively, bacteriocins of B. thuringiensis inhibited the growth of different bacteria tested here and they could have a viable potential for use in integrated management programs to control or prevent mastitis in animals.…”

Section: Discussionmentioning

confidence: 60%

Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico

León-Galván

Barboza‐Corona

Lechuga-Arana

et al. 2015

BioMed Research International

View full text Add to dashboard Cite

Thirty-two farms (n = 535 cows) located in the state of Guanajuato, Mexico, were sampled. Pathogens from bovine subclinical mastitis (SCM) and clinical mastitis (CLM) were identified by 16S rDNA and the sensitivity to both antibiotics and bacteriocins of Bacillus thuringiensis was tested. Forty-six milk samples were selected for their positive California Mastitis Test (CMT) (≥3) and any abnormality in the udder or milk. The frequency of SCM and CLM was 39.1% and 9.3%, respectively. Averages for test day milk yield (MY), lactation number (LN), herd size (HS), and number of days in milk (DM) were 20.6 kg, 2.8 lactations, 16.7 animals, and 164.1 days, respectively. MY was dependent on dairy herd (DH), LN, HS, and DM (P < 0.01), and correlations between udder quarters from the CMT were around 0.49 (P < 0.01). Coagulase-negative staphylococci were mainly identified, as well as Staphylococcus aureus, Streptococcus uberis, Brevibacterium stationis, B. conglomeratum, and Staphylococcus agnetis. Bacterial isolates were resistant to penicillin, clindamycin, ampicillin, and cefotaxime. Bacteriocins synthesized by Bacillus thuringiensis inhibited the growth of multiantibiotic resistance bacteria such as S. agnetis, S. equorum, Streptococcus uberis, Brevibacterium stationis, and Brachybacterium conglomeratum, but they were not active against S. sciuri, a microorganism that showed an 84% resistance to antibiotics tested in this study.

show abstract

“…Blood of each mouse was collected and the serum samples obtained by centrifugation were kept frozen at -20 o C. The entire spleen and thymus of mice were promptly removed after killed and weighed. The spleen and thymus indexes were calculated according to the formulas: (spleen or thymus weight / body weight) × 100% [23].…”

Section: Samples Collection and Detection Of Thymus And Spleen Indexesmentioning

confidence: 99%

“…When pathogenic microorganisms invade the mammary gland via the teat orifice and cause an inflammation of the secretory tissue, manifesting itself in either clinical or sub-clinical mastitis [29]. Over 200 agents have been recorded to cause bovine mastitis [2], but the vast majority of cases were caused by Staphylococcus aureus, Streptococcus dysgalactiae, Streptococcus agalactiae, Escherichia coli and Streptococcus uberis [15,17,23].…”

Section: Introductionmentioning

confidence: 99%

Immune Responses in Mice Immunized with Mastitis Multiple Vaccines Using Different Adjuvants

Zhang

Yang

et al. 2018

Acta Scientiae. Vet.

View full text Add to dashboard Cite

Background: Bovine mastitis, a serious disease associated with both high incidence and significant economic losses, posing a major challenge to the global dairy industry. The development of vaccines for protection from new infections by mastitis pathogens is of considerable interest to the milk production industry. Vaccination is a common and easy strategy for the control of infectious diseases, and the adjuvants used in the formulation is a critical factor for vaccine efficacy improvement. The main objective of the present study was to evaluate three different adjuvants for their ability to enhance immune responses of mice that vaccinated with Bovine Mastitis Multiple Vaccine.Materials, Methods & Results: The thymus and spleen index, the phagocytic ability of macrophage and the serum antibody levels of mice were detected after vaccination, respectively. The results showed that the thymus index, spleen index, and the phagocytic ability of macrophage of mice in Aluminum group exhibited a significant higher level (P < 0.05) compared with those in the control groups. The difference of the serum antibody levels was significant (P < 0.05) between experimental groups and control group after vaccination. The serum antibody concentration of mice in FIA group was higher compared with other groups and had a longer duration. The antibody concentration of mice in France 206 oil group can not increase as fast as the antibody concentration of Aluminum group, but it can last a longer time at a high level. In conclusion, multiple vaccines mixed with three different adjuvants could enhance the immunity of mice and Freund’s incomplete adjuvant is the best choice for this vaccine.Discussion: Adjuvants play an important role in increasing the efficacy of a number of different vaccines. In this study, three kinds of adjuvants (Aluminum hydroxide, France 206 oil and FIA) were evaluated for their adjuvant effects for multiple vaccine of bovine mastitis in mice and aluminum hydroxide did best as the vaccine adjuvant from the results. Aluminum hydroxide is a universally accepted adjuvant for both human and veterinary vaccines. The goal of vaccination is to generate strong immune response providing protection against infection for a time. Different protective effects will usually obtained by different adjuvants even use same antigen. In this work, FIA, Alum and 206 oil were chosen as adjuvants for inactivated antigens of Streptococcus agalactiae, Streptococcus dysgalactiae and Staphylococcus aureus. The results showed that there was a significantly higher antibody levels in vaccinated mice compared with those in control group. In addition, the mice in France 206 oil and FIA group performed a higher antibody levels and stronger immunity than mice in Aluminum hydroxide groups. These findings suggest that Freund’s incomplete adjuvant (FIA) would be the best candidate as the adjuvant for mastitis multiple vaccines investigated in this study.

show abstract

Prevalence of subclinical mastitis and isolated udder pathogens in dairy cows in Southern Vietnam

Cited by 31 publications

References 25 publications

Prevalence and Economic Impacts of Retained Placenta and Subclinical Mastitis in Tanzanian Dairy Cows

Prevalence and Economic Impacts of Retained Placenta and Subclinical Mastitis in Tanzanian Dairy Cows

Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico

Immune Responses in Mice Immunized with Mastitis Multiple Vaccines Using Different Adjuvants

Contact Info

Product

Resources

About