Prevalence of the lpfO113 gene cluster among Escherichia coli O157 isolates from different sources

Osek, Jacek; Weiner, Marcin; Hartland, Elizabeth L.

doi:10.1016/j.vetmic.2003.07.002

Cited by 27 publications

(30 citation statements)

References 31 publications

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“…The E. coli O157 phenotypic properties listed in Table 1 were confirmed earlier by the identification of the respective genes using PCR (Osek, 1999(Osek, , 2002Osek et al, 2000Osek et al, , 2003. There was a 100% correlation between the results of the phenotypic analyses performed in this study and the results of the previous genotypic tests.…”

Section: Characteristics Of E Coli Strains Testedsupporting

confidence: 80%

“…There was a 100% correlation between the results of the phenotypic analyses performed in this study and the results of the previous genotypic tests. Moreover, in the present results, two additional genetypic markers (eaeA and lpf O113 ) responsible for the expression of E. coli O157 a�aching properties were included (Osek et al, 2000(Osek et al, , 2003. It was found that all 23 isolates of human origin and the majority of strains (26; 89.6%) recovered from ca�le were intimin gene positive.…”

Section: Characteristics Of E Coli Strains Testedmentioning

confidence: 46%

“…PCR analyses. All PCR tests were performed before and their results had been published previously (Osek, 1999(Osek, , 2002Osek et al, 2000Osek et al, , 2003. Briefly, E. coli strains were grown overnight at 37°C on LB agar.…”

Section: Methodsmentioning

confidence: 99%

“…The resulting supernatant was used as a template for PCR. The following genes were amplified: r�O157 (E. coli O157), fliC (H7), stx1 and stx2 (Shiga toxin 1 and 2, respectively), stx2e (Shiga toxin 2e variant), ehly (enterohemolysin), eaeA (intimin), and lpf O113 (long polar fimbriae) using the PCR primers and amplification conditions as described previously (Osek, 1999(Osek, , 2002Osek et al, 2000Osek et al, , 2003.…”

Section: Methodsmentioning

confidence: 99%

“…a Phenotypic properties were tested as described in Material and Methods. The respective genes were analysed previously by PCR (Osek, 2002;Osek et al, 2003) b Additional 20 strains possessed genes encoding Stx2e toxin variant as determined by PCR (Osek, 1999) It was demonstrated that Shiga toxins 1 and 2 were produced by E. coli O157 strains isolated from all 3 sources (humans, ca�le, pigs) (Table 1). It was found that all human and bovine isolates could be classified to the STEC group and were able to produce either one toxin (Stx1 or Stx2) alone or both.…”

Section: Characteristics Of E Coli Strains Testedmentioning

confidence: 99%

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Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Osek¹

2004

Vet. Med. - Czech

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A total of 90 Escherichia coli O157 isolates recovered from humans, cattle, and pigs, were examined for the presence of the H7 antigen, ability to produce Shiga toxins and enterohemolysin as well as for antimicrobial resistance and biochemical properties. Fourteen of the human strains (n = 23) and 21 of the bovine isolates (n = 29) were of the O157:H7 serotype as determined by agglutination and PCR methods. All E. coli O157 of porcine origin (n = 38) were H-negative. Based on the ability to produce Shiga toxins (Stxs), all human and cattle isolates and 11 strains recovered from swine were identified as Shiga toxin-producing E. coli (STEC). Among STEC, most human strains (18 isolates) were Stx1- and Stx2-positive whereas cattle strains were mostly Stx2-positive. Eleven porcine STEC produced either Stx1 (7 isolates) or Stx2 (4 strains) toxins; an additional 20 isolates recovered from these animals had the Stx2e toxin gene as previously determined by PCR. All human and cattle E. coli O157 produced enterohemolysin whereas only 4 strains recovered from pigs were ehly-positive. Moreover, the PCR identification of the lpf<sub>O113</sub> gene performed earlier revealed that this putative virulence marker was present in all porcine isolates, only in 5 strains of bovine origin but in none of E. coli O157 recovered from humans. All 90 E. coli O157 strains tested displayed 10 biochemical profiles that were different at least in one of the reaction tested. The most common atypical reaction observed among porcine O157 isolates was ability to ferment sorbitol (all strains) and production of β-glucuronidase (25 isolates). Moreover, none of the sorbitol-positive strains was able to produce indol. Four antimicrobial resistance profiles among 90 E. coli O157 strains tested were observed. Most of the isolates recovered from humans and all strains from cattle were resistant only to rifampicin whereas the porcine strains showed resistance to either 3 antimicrobials (4 isolates) or to 4 drugs tested (34 isolates). The phenotypic data shown in the present study, together with the previously published genotypic analyses of these strains, confirm earlier suggestions that the porcine E. coli O157 strains are mostly different from those of bovine and human O157 isolates and could therefore play less important role in human STEC O157 infections.

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Section: Characteristics Of E Coli Strains Testedsupporting

confidence: 80%

Section: Characteristics Of E Coli Strains Testedmentioning

confidence: 46%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Characteristics Of E Coli Strains Testedmentioning

confidence: 99%

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Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Osek¹

2004

Vet. Med. - Czech

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Characterization of the second long polar (LP) fimbriae of O157:H7 and distribution of LP fimbriae in other pathogenic strains

Torres

Kanack

Tutt

et al. 2004

FEMS Microbiology Letters

122

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A second region containing five genes homologous to the long polar fimbrial operon of Salmonella enterica serovar Typhimurium is located in the chromosome of enterohemorrhagic Escherichia coli (EHEC) O157:H7. A non-fimbriated E. coli K-12 strain carrying the cloned EHEC lpf (lpf2) genes expressed thin fibrillae-like structures on its surface and displayed reduced adherence to tissue culture cells. Neither mutation in the lpfA2 gene in either the parent or lpfA1 mutant strains showed an effect in adherence or in the formation of A/E lesions on HeLa cells. lpfA2 isogenic mutant strains adhere to Caco-2 cells almost as well as the wild-type at 5 h, but they were deficient in adherence at early time points. A collection of diarrheagenic E. coli strains were investigated for the presence of lpfA1 and lpfA2 and results showed that these genes are present in specific serogroups which are phylogenetically related. Our results suggest that LP fimbriae 2 may contribute to the early stages of EHEC adhesion and that genes encoding the major LP fimbrial subunits are present in a small group of EHEC and EPEC serotypes.

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Identification of putative adhesin genes in shigatoxigenic Escherichia coli isolated from different sources

Tatarczak

Wieczorek

Possé

et al. 2005

Veterinary Microbiology

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Prevalence of the lpfO113 gene cluster among Escherichia coli O157 isolates from different sources

Cited by 27 publications

References 31 publications

Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Characterization of the second long polar (LP) fimbriae of O157:H7 and distribution of LP fimbriae in other pathogenic strains

Identification of putative adhesin genes in shigatoxigenic Escherichia coli isolated from different sources

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