Prevalence of ticks (Acari: Ixodidae) and Theileria lestoquardi in sheep at El Huda and El Nuhud animals production research stations, Sudan

Magzoub, Abdelmonim; Ghali, Ahmed El; Hussien, Mohammed; Juma, Yasir Juma Adam; Mohammed, S. B.

doi:10.1007/s12639-020-01284-8

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…Regarding sex as a studied individual variable factor, this study found that the overall prevalence of blood parasites in male sheep was significantly higher compared to females, which aligns with earlier studies conducted nationally or internationally ( 63 – 66 ). However, the present findings are in contrast with another reports in Egypt ( 31 , 33 , 62 ).…”

Section: Discussionsupporting

confidence: 89%

“…was higher in cold season (44.6% and 21.4%, respectively) than in hot season (11.4% and 4.5% respectively). Similarly, another previous study detected higher prevalence of blood parasites in winter when compared to summer ( 63 ). The obtained result disagreed with those data recorded ( 4 ) in Behera governorate (Lower Egypt), who reported higher infection rate in summer months as compared to winter season.…”

Section: Discussionsupporting

confidence: 55%

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Microscopic and molecular detection of piroplasms among sheep in Upper Egypt

Dyab,

Mohamed,

Abdel-Aziz

et al. 2024

Front. Vet. Sci.

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IntroductionBlood parasites pose a significant threat to livestock production in southern Egypt, yet there is a scarcity of information regarding their circulation and epidemiology in sheep in this region. This study aimed to investigate the seroprevalence of blood parasite infections in sheep in Assiut governorate, Upper Egypt.MethodsA total of 400 blood samples were collected from sheep of varying ages and genders. The preliminary screening for the presence of piroplasms, mainly Babesia and Theileria spp., via microscopic examination, followed by investigation of the potential risk factors linked with the exposure to infection. Moreover, molecular identification of both parasites on some of positive samples was performed using PCR targeting Babesia 18S rRNA and Theileria annulata Tams1 gene.ResultsThe microscopic examination revealed that among the examined sheep, there was an overall prevalence of blood parasites at 44% (176 out of 400), with Babesia spp. observed in 14% (56 out of 400) and Theileria spp. in 30% (120 out of 400). Furthermore, the infection rate was non-significantly higher in young animals (50%) compared to adults (38.5%) (P = 0.246). Male sheep exhibited a significantly higher vulnerability to both parasites' infection (63.3%) compared to females (35.7%) (P = 0.011). Interestingly, the prevalence of both blood parasites was significantly higher during the cold season (66.1%) compared to the hot season (15.9%) (P = < 0.001). The molecular analysis identified the presence of Babesia ovis and Theileria annulata among a subsample of the positive sheep's bloods films. The identified species were recorded in the GenBank™ databases and assigned specific accession numbers (OQ360720 and OQ360719 for B. ovis), and (OP991838 for T. annulata).ConclusionsTaken together, this study confirms a high prevalence of piroplasmosis and offers epidemiological and molecular insights into blood parasites in sheep from Upper Egypt, highlighting the importance of detecting these parasites in various hosts and their competent vectors (ticks).

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 55%

Microscopic and molecular detection of piroplasms among sheep in Upper Egypt

Dyab,

Mohamed,

Abdel-Aziz

et al. 2024

Front. Vet. Sci.

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“…In a study by(Al- HamidhiWeir et al 2016) respectively. The results of the present study on the determination of Theileria species with the results of similar studies indicate the high sensitivity of molecular methods compared to microscopic method(Magzoub et al 2021).…”

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confidence: 78%

First Molecular Evidence of Theileria Lestoquardi in Small Ruminants in Northern Iran

Haghi

Fakhar

Sharbatkhori

et al. 2021

Preprint

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Ovine theileriosis as a critical agent in small ruminant production, can cause lethal infections. Different species of Theileria have been reported in various parts of the world, and each species causes different diseases in the host. This is the first molecular study to investigate the prevalence of ovine theileriosis and identify the dominant Theileria species in northern Iran. A number of 220 small ruminants, including sheep and goats, were randomly sampled from 22 flocks. Peripheral blood smears are stained by the Giemsa staining method. As well as for species identification, all samples were examined by molecular method. From 220 samples, 160 and 60 were sheep and goat, respectively. By the Giemsa staining method, Theileria parasite was observed in 20 (9%) samples. But by polymerase chain reaction (PCR) method, 30 (13.6%) samples were positive for Theileria species. Theileria lestoquardi was the most common species found in these animals. The high prevalence of theileriosis in small ruminants demonstrates the emergence of ovine theileriosis in Mazandaran and Golestan provinces in northern Iran.

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“…annulate and Th. lestoquardi from domestic animals in Sudan [ 31 , 32 ]. Other studies reported that Th.…”

Section: Discussionmentioning

confidence: 99%

Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels

et al. 2021

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Dromedary camels (Camelus dromedarius) are widely distributed in Africa, the Middle East and northern India. In this study, we aimed to detect tick-borne pathogens through investigating prokaryotic and eukaryotic microorganisms in camel blood based on a metagenomic approach and then to characterize potentially pathogenic organisms using traditional molecular techniques. We showed that the bacteria circulating in the blood of camels is dominated by Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria. At the genus level, Sediminibacterium, Hydrotalea, Bradyrhizobium and Anaplasma were the most abundant taxa. Eukaryotic profile was dominated by Fungi, Charophyta and Apicomplexa. At the genus level, Theileria was detected in 10 out of 18 samples, while Sarcocystis, Hoplorhynchus and Stylocephalus were detected in one sample each. Our metagenomic approach was successful in the detection of several pathogens or potential pathogens including Anaplasma sp., Theileria ovis, Th. separata, Th. annulate, Th. mutans-like and uncharacterized Theileria sp. For further characterization, we provided the partial sequences of citrate synthase (gltA) and heat-shock protein (groEL) genes of Candidatus Anaplasma camelii. We also detected Trypanosoma evansi type A using polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) region. This combined metagenomic and traditional approach will contribute to a better understanding of the epidemiology of pathogens including tick-borne bacteria and protozoa in animals.

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Prevalence of ticks (Acari: Ixodidae) and Theileria lestoquardi in sheep at El Huda and El Nuhud animals production research stations, Sudan

Cited by 4 publications

References 22 publications

Microscopic and molecular detection of piroplasms among sheep in Upper Egypt

Microscopic and molecular detection of piroplasms among sheep in Upper Egypt

First Molecular Evidence of Theileria Lestoquardi in Small Ruminants in Northern Iran

Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels

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