Prevalence of Toxoplasma gondii indirect fluorescent antibodies in naturally- and experimentally-infected chickens (Gallus domesticus) in Thailand

Chumpolbanchorn, Kamlang; Anankeatikul, Pacharaporn; Ratanasak, Wantanee; Wiengcharoen, Jitbanjong; Thompson, R.C.A.; Sukthana, Yaowalark

doi:10.2478/s11686-009-0034-2

Cited by 10 publications

(6 citation statements)

References 7 publications

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“…Our results showed much lower than the previous study in Thailand that reported the prevalence by IFAT of 64% by IFAT in 1-year chickens from Bangkok [14]. A study from Kenya [15] also reported high prevalence as 79.0% in 2-years chickens.…”

Section: Discussioncontrasting

confidence: 56%

“…In this study, both IFAT and MAT were used and able to detect T. gondii antibodies in all inoculated chickens. IFAT 7 T. gondii tachyzoites (RH strain) injected muscularly [14], or fed with 10 3 or 10 5 T. gondii oocysts K7 (avirulent) strains [29]. Similarly, after chickens was fed with 10 3 or 10 5 of ME49 oocysts, MAT was able to detect antibody at 1:100 serum dilutions at day 15 post-inoculation [30].…”

Section: Discussionmentioning

confidence: 99%

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Toxoplasma Serostatus in Thai Free- Range Chickens: Prevalence and Two Diagnostic Methods

Udonsom¹,

Chaichan²,

Mahittikorn³

et al. 2017

Open J Trop Med

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Toxoplasma seroprevalence in free-range chicken, which directly feed from ground, is a good indicator for detecting an environment contamination by T. gondii oocysts. Many serological methods have been used for diagnosing Toxoplasma infection in animals including chicken. Selection of appropriate methods primarily depend on their sensitivity and specifi city as well as laboratory techniques, special reagent or tool requirements and regional prevalence status. The aims of this study were to determine the prevalence of T. gondii antibody in free-range chickens in Thailand and to compare two serodiagnostic methods, indirect fl uorescent antibody test (IFAT) and Modifi ed Agglutination Test (MAT). Six hundred free-range chicken sera were collected from 2 villages in Kanchanaburi Province, Thailand, 300 in 2014 and 300 in 2015. The age of chickens was between 3-6 mo, of which chickens of 2015 were older than of 2014. Collected sera were test for T. gondii IgG antibody by both methods. Over all Toxoplasma seroprevalence were 33.0% and 17.7% by IFAT and MAT, respectively. There was poor agreement between the two serological methods (kappa=0.09). IFAT in this study revealed higher positive percentage than MAT. Even though the way of living and environment in the present study was suitable for Toxoplasma transmission, the seroprevalence in Thai free-range chickens was low compared to other tropical regions such as Africa or South America. A low Toxoplasma prevalence was also found in humans, cats and other intermediate hosts in Thailand. Toxoplasmosis in Thailand causes low burden compare to other infectious diseases, however, consume well-cooked free-range chicken meat and proper practice during handling raw meat when cooking should be advocated to prevent and reduce this infection.

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Section: Discussioncontrasting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Toxoplasma Serostatus in Thai Free- Range Chickens: Prevalence and Two Diagnostic Methods

Udonsom¹,

Chaichan²,

Mahittikorn³

et al. 2017

Open J Trop Med

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“…This is in accord with observations from previous studies in chickens and turkeys (Biancifiori et al . 1986; Dubey et al 1993 b ; Kaneto et al 1997; Sedlak et al 2000; Chumpolbanchorn et al 2009; Zöller et al 2013) where subclinical toxoplasmosis in experimentally infected birds was seen. However, Dubey et al (1993 b ) described cases of death due to acute toxoplasmosis in chickens after inoculation of a high dose (100 000 oocysts) of the T. gondii GT1 strain.…”

Section: Discussionmentioning

confidence: 99%

Persistence ofToxoplasma gondiitissue stages in poultry over a conventional fattening cycle

Geuthner¹,

Koethe²,

Ludewig³

et al. 2014

Parasitology

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Toxoplasma gondii is a widely spread protozoon in humans, mammals and poultry. Regarding the latter, nothing is known yet about the duration of T. gondii persistence and distribution over a conventional fattening cycle of turkeys and chickens. Twenty-four turkeys and 12 broiler chickens were infected intravenously with 1×10(6) T. gondii tachyzoites (strain NED). Serum antibody levels were determined weekly by ELISA (turkeys) or immunofluorescent antibody test (chickens). Turkeys were slaughtered at 4, 8, 12 and 16 weeks post-infection (p.i.), and chickens 5 or 10 weeks p.i. (n = 6 per group). Sixteen different tissue samples per bird were analysed for T. gondii by PCR. All infected animals showed seroconversion. In turkeys, 15.9% of all samples were tested positive for T.-gondii-DNA. Among the edible tissues (drumstick, thigh, breast muscle, heart, liver and gizzard) 7.8% tested positive. Among poultry slaughtered after different periods of time after infection no significant differences (P>0.05) regarding the number of positive samples were observed. Only 4 out of 192 samples (2.1%) from infected chickens contained detectable T. gondii DNA.The PCR findings suggested that T. gondii may persist in poultry. Particularly in turkey it was shown that edible tissues stay infected for at least 16 weeks p.i. which indicates a potential risk for consumers of undercooked turkey meat whereas chickens appear less susceptible to T. gondii infection.

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“…Chickens inoculated intravenously with T. gondii tachyzoites generally remained asymptomatic, irrespective of the dose (Chumpolbanchorn et al ., 2009; Geuthner et al ., 2014; Schares et al ., 2017 a , b ). Chickens orally inoculated with oocysts can develop diarrhoea, and the effect may be neurogenic rather than the destruction of enterocytes (Bonapaz et al ., 2010; Braga et al ., 2011).…”

Section: Experimental Infectionsmentioning

confidence: 99%

Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade

Dubey¹,

Pena²,

Cerqueira‐Cézar³

et al. 2020

Parasitology

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Toxoplasma gondii infections are common in humans and animals worldwide. Domestic free-range chickens (Gallus domesticus) are excellent sentinels of environmental contamination with T. gondii oocysts because they feed on the ground. Chickens can be easily infected with T. gondii; however, clinical toxoplasmosis is rare in these hosts. Chickens are comparatively inexpensive and thus are good sentinel animals for T. gondii infections on the farms. Here, the authors reviewed prevalence, the persistence of infection, clinical disease, epidemiology and genetic diversity of T. gondii strains isolated from chickens worldwide for the past decade. Data on phenotypic and molecular characteristics of 794 viable T. gondii strains from chickens are discussed, including new data on T. gondii isolates from chickens in Brazil. This paper will be of interest to biologists, epidemiologists, veterinarians and parasitologists.

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Prevalence of Toxoplasma gondii indirect fluorescent antibodies in naturally- and experimentally-infected chickens (Gallus domesticus) in Thailand

Cited by 10 publications

References 7 publications

Toxoplasma Serostatus in Thai Free- Range Chickens: Prevalence and Two Diagnostic Methods

Toxoplasma Serostatus in Thai Free- Range Chickens: Prevalence and Two Diagnostic Methods

Persistence ofToxoplasma gondiitissue stages in poultry over a conventional fattening cycle

Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade

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