A total of 210 specimens were collected from burn wound patients their ages ranged from 5-50 years during the period March to August 2016 from inpatients whom attended to hospitals in Baghdad /Iraq. The bacterial agents were showed that single pathogens growth isolates only 42 isolates (37.5 %) of Klebsiella pneumoniae , 36 isolates (32.14%) Pseudomonas spp., 20 isolates (17.86%) Escherichia coli, 10 isolates (8.93%) Staphylococcus aureus and 4 isolates (3.57%) proteus spp. .While mixed growth isolates frequency showed K. pneumoniae and Pseudomonas spp.64 isolates (65.31 %) , Pseudomonas spp. and E. coli 18 isolates (18.37%), K. pneumoniae and E. coli 7 isolates (7.14%), Pseudomonas spp. and proteus spp. 4 isolates (4.08 %) , K. pneumoniae and Staph. aureus 3 isolates (3.06 %) and proteus spp. and E. coli 2 isolates (2.04 %). The results susceptibility testing showed that frequency resistance of K. pneumoniae isolates to: doxycycline were (100 %) ,tetracycline (95.23%) , cefotaxime and pipracillin (85.71%) ceftriaxone (88.09%), trimethoprim sulfamethoxazol (83.33%) , ticarcillin (78.57%), aztreonam (71.2%) , ceftazidime (69.04%) , ciprofloxacin (59.52%), gentamycin (26.19%) , imipenem (21.42%) , meropenem and amikacin(19.04 %). Also the results showed the highest frequency of susceptibility among K. pneumoniae was associated to gentamycin (78.57%) , meropenem and amikacin (76.19%) and imipenem (69.04%).