Prevalence of Tritrichomonas foetus and Campylobacter fetus subsp. venerealis among bulls slaughtered in the state of Minas Gerais, Brazil

Abstract

Bovine genital campylobacteriosis (BGC) and bovine genital trichomonosis (BGT) are globally widespread, predominantly sexually transmitted diseases that mainly affect herds in which natural mating takes place. Bulls serve as good epidemiological indicators for studying the causative agents of these diseases in cattle herds. The objective of the present work was to determine the prevalence of BGC and BGT among bulls sent to slaughterhouses in southern Minas Gerais, Brazil. Samples of preputial smegma from 200 sexually mature, non-castrated bulls of several ancestries were collected in 2013 from four regional slaughterhouses. The polymerase chain reaction (PCR) was employed to detect Tritrichomonas foetus, Campylobacter fetus subsp. fetus, and C. fetus subsp. venerealis. Isolation also was used aiming to diagnosis of BGT. All smegma samples were negative in culture for T. foetus. Molecular tests revealed 8% (16/200) of animals to be positive for T. foetus, 17.5% (35/200) for Campylobacter. fetus subsp. fetus, and 13.5% (27/200) for C. fetus subsp. venerealis. The results of the present study indicate that T. foetus, C. fetus subsp. venerealis, and C. fetus subsp. fetus are present among bulls slaughtered in southern Minas Gerais, and that BGC and BGT occur in this region. These diseases therefore need to be considered during differential diagnosis of reproductive diseases affecting cattle herds in which natural mating is employed.

Investigação de Campilobacteriose e Tricomonose genital em touros de propriedades rurais do Pantanal Mato-Grossense, Brasil

“…These methods are highly specific and sensitive, independent of pathogen viability (Groff et al, 2010;Carli et al, 2020). They do not require extensive precautions during sample transportation since the DNA remains intact even if the agent is nonviable, overcoming the limitations of traditional diagnostic methods (Botelho et al, 2018).…”

“…In recent years, PCR has been increasingly used as a diagnostic tool for the direct detection of microorganisms in samples (Van der Graaf-Van Bloois et al, 2013;Carli et al, 2020). PCR offers high sensitivity and specificity (Groff et al, 2010) and does not require extensive precautions during sample transportation, as the DNA remains intact even if the pathogen is non-viable (Botelho et al, 2018).…”

Doenças Infecciosas Reprodutivas Que Acometem Vacas De Corte

“…(ROCHA,2021) Essas e outras doenças reprodutivas doenças diminuem a produtividade nas propriedades tal como a vida útil dos animais, reduz a lucratividade, aumenta o custo de tratamento, aumenta o descarte de animais, diminui o desempenho das matrizes que por consequência diminui a produção de bezerros e com isso acaba acarretando um grande impacto econômico. (BOTELHO et al, 2018;ZANATTO et al, 2019).…”

“…Essas doenças diminui a produtividade nas propriedades tal como a vida útil dos animais, reduz a lucratividade, aumenta o custo de tratamento, aumenta o descarte de animais, o que gera uma competitividade entre os bens produzidos, o que acaba acarretando um grande impacto econômico. Essas doenças reprodutivas diminui o desempenho das matrizes que por consequência diminui a produção de bezerros (BOTELHO et al, 2018;ZANATTO et al, 2019).…”

Survey of beef bulls in Brazil to assess their role as source of infectious agents related to cow infertility

“…venerealis , Ureaplasma diversum , Mycoplasma bovigenitalium , and Mycoplasma bovis , 2,5,22,28 and the protozoan Tritrichomonas foetus . 6 In addition, the viruses bovine viral diarrhea virus (BVDV; Flaviviridae , Pestivirus A ) and bovine herpesvirus 1 (BoHV1; Herpesviridae , Varicellovirus , Bovine alphaherpesvirus 1 ) have an important role in bovine reproductive losses. 38…”