Prevalence of Trypanosoma sp. in cattle from Tanzania estimated by conventional PCR and loop-mediated isothermal amplification (LAMP)

Abstract

This study compared the prevalence of trypanosome infections estimated by PFR-loop-mediated isothermal amplification (LAMP) with conventional polymerase chain reaction (PCR) tests. One hundred forty eight cattle blood samples were collected from Robanda village, Mara region, Tanzania in April 2008. In conventional PCR, four sets of primers, specific for the detection of Trypanosoma sp., Trypanosoma brucei rhodesiense, Trypanosoma vivax, and Trypanozoon, as well as a modified LAMP were used. Conventional PCR detected no infection or up to 8, 1, and 3 infections with Trypanosoma congolense savannah, Trypanozoon, and T. vivax, respectively, whereas LAMP detected additional 44 Trypanozoon positive cases. Our results clearly indicate that the prevalence of Trypanozoon spp. in cattle in Robanda village estimated by PFR-LAMP (30.4%) was significantly higher than the estimates by PCR assays (0.6-2%). As such, future studies should target epidemiological surveys of Trypanozoon and T. brucei rhodesiense infections in possible reservoir animals by LAMP to further elucidate the actual prevalence of these parasites.

A TeGM6-4r antigen-based immunochromatographic test (ICT) for animal trypanosomosis

“…Moreover, microscopic examination of the buffy coat detected five samples that were positive for both T. congolense and T. vivax. This result is consistent with other reports which indicate that co-infection of animal trypanosomosis is present in African cattle (Gillingwater et al 2010;Laohasinnarong et al 2011). Since the therapeutic treatment for different animal trypanosomosis are quite similar, the TeGM6-4r/ICT, which can detect several pathogenic trypanosomes, represents a good choice of test.…”

Preliminary Investigation of Trypanosomosis in Exotic Dog Breeds from Zambia's Luangwa and Zambezi Valleys Using LAMP

“…Although trypanosomosis is commonly reported in farm animals, 4 canine African trypanosomosis (CAT) is rarely reported in the literature. 3,5 This communication evaluates the performance of loop-mediated isothermal amplification (LAMP) in detecting CAT from six exotic dogs naturally infected with trypanosomes from Zambia's South Luangwa National Park (SLNP) and Chiawa Game Management Area (CGMA), against routine microscopy.…”

“…In agreement with previous reports, 3 T. congolense-infected dogs tended to exhibit chronic infections unlike those infected with T. brucei subspecies that showed more acute symptoms. Such chronically infected dogs may act as a source of infection to other domestic animals 4 in tsetseinfested regions.…”

Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection

“…This technique has been proven to be an accurate, rapid and simple method, which amplifies the target nucleic acid under isothermal conditions (Notomi et al, 2000). Recently, wide applicability of LAMP in the detection of parasitic protozoa such as Babesia, Plasmodium, Leishmania and Trypanosoma in clinical samples has been demonstrated (Ikadai et al, 2004;Poon et al, 2006;Njiru et al, 2008;Takagi et al, 2009;Thekisoe et al, 2007;Laohasinnarong et al, 2011). Studies have also shown the application of LAMP to survey vectors of infectious diseases (Aonuma et al, 2009;Thekisoe et al, 2010;Nakao et al, 2010); however, these studies used purified DNA as a template for the LAMP assays.…”