Prevalence of various Beta-lactamases in Enterobacteriaceae in a tertiary care hospital in South India: A Cross-sectional study

S, Roopashree; Kaup, Soumya

doi:10.18231/j.ijmmtd.2021.039

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…Sample size was calculated based on the prevalence of ESBL among Enterobacteriaceae as reported by Kaup S et al (54.79%). 2 Total 170 strains of Enterobacteriaceae showing resistance to two or more classes of antimicrobials were further processed.…”

Section: Sample Sizementioning

confidence: 99%

“…1 The infections caused by MDR organisms are increasing gradually and pose a threat to treatment thereby leading to extended period of hospitalization, increased cost of health care and increased rate of morbidity and mortality. 2 Infections associated with ESBL producing organisms can be treated with third or fourth generation cephalosporins combined with clavulanic acid. 2 However, if organism is an Amp C enzyme producer along with ESBL, patient fails to respond to clavulanic acid combination.…”

Section: Introductionmentioning

confidence: 99%

“…2 Infections associated with ESBL producing organisms can be treated with third or fourth generation cephalosporins combined with clavulanic acid. 2 However, if organism is an Amp C enzyme producer along with ESBL, patient fails to respond to clavulanic acid combination. In fact, clavulanic acid induces the production of Amp C enzymes and causes more harm.…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic detection of ESBL and Amp C beta lactamase among clinical isolates of Enterobacteriaceae

Thakar,

Gandavalli,

Devhare

et al. 2024

IJMMTD

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Emergence of ESBL & amp C β-Lactamase enzymes among clinical isolates of poses a significant public health concern. Antimicrobial agents like 3 generation cephalosporins, clavulanic acid, imipenem etc. can induce amp C enzyme production. Sometimes the automated antimicrobial susceptibility testing systems fails to detect Amp C phenotype due to presence of multiple resistance mechanisms in gram negative bacilli. To overcome such difficulty, the study was undertaken to detect the presence ESBL and Amp C enzymes among clinical isolates of by phenotypic methods. strains isolated from various clinical samples were included in the study. Strains resistant to two or three groups of antibiotics (MDR) were further tested for the presence of ESBL enzyme by combination disk method. Amp C enzymes were detected by cefoxitin- cloxacillin disc method.A total1059 strains were isolated from various clinical samples. Out of these, 170 MDR strains were further processed. ESBL enzymes were detected in 104 (61%) strains and Amp C in 35 (20.5%) strains. 26(15.2%) strains were co-producers. Detection of ESBL and Amp C enzymes will help clinician in choosing the right antimicrobial treatment for the patient. Routine reporting of presence of Amp C & ESBL enzymes by phenotypic methods can be easily implemented by clinical microbiology laboratory. Cefoxitin-cloxacillin disk test is simple & rapid method for detection of Amp C β- lactamase enzyme.

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Section: Sample Sizementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenotypic detection of ESBL and Amp C beta lactamase among clinical isolates of Enterobacteriaceae

Thakar,

Gandavalli,

Devhare

et al. 2024

IJMMTD

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Modified Carbapenem Inactivation Method and Ethylenediaminetetraacetic Acid (EDTA)-Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Enterobacterales and Pseudomonas aeruginosa

Verma,

Singh,

Smriti

et al. 2024

Cureus

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Introduction: The rising incidence of carbapenem resistance in Enterobacterales and Pseudomonas aeruginosa is a concern. Since carbapenemase production is the primary resistance mechanism, detecting and identifying the genes responsible for it is crucial to effectively monitor its spread. Objective: This study aims to detect positivity for the modified carbapenem inactivation method (mCIM) and ethylenediaminetetraacetic acid (EDTA)-carbapenem inactivation method (eCIM) for the detection of carbapenemase-producing Enterobacterales and Pseudomonas aeruginosa . Methods: Methods: A cross-sectional study was carried out at a tertiary care hospital, including 250 clinical isolates of Enterobacterales and Pseudomonas aeruginosa . These isolates exhibited resistance to at least one of the carbapenems as determined by the VITEK AST 2 System (bioMérieux, USA). The isolates were subjected to mCIM testing, and those that tested positive were further tested using eCIM. The results were interpreted in accordance with the guidelines provided by the Clinical and Laboratory Standards Institute (CLSI) 2023. Results: Out of the total 250 carbapenem-resistant Enterobacterales and Pseudomonas aeruginosa isolates, 151 (60.4%) were Klebsiella pneumonia, 44 (17.6%) were Escherichia coli, 10 (4.0%) were Enterobacter cloacae , 6 (2.4%) were Providencia spp ., 4 (1.6%) were Serratia marcescens , 4 (1.6%) were Proteus mirabilis and 31 (12.4%) were Pseudomonas aeruginosa . Positivity for the mCIM was observed in 96% (240 out of 250) of the isolates. Of the mCIM-positive isolates, 234 (97.5%) also tested positive for eCIM, indicating metallo-β-Lactamase (MLB) production. A statistically significant association was found between both mCIM and eCIM positivity and the degree of resistance to carbapenem (p<0.05) . Conclusion: This study shows that the inexpensive method, a combination of mCIM and eCIM assists in differentiating between serine carbapenemase producers and MLB producers, thereby guiding the selection of appropriate therapy and useful in infection control in resource-limited settings.

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An Insight Into the Bacteriological Profile: Escalating Antimicrobial Resistance Patterns and the Spectrum of Multidrug-Resistant Enterobacteriaceae at a Tertiary Care Center in Western Maharashtra

Sharma,

Karande,

Patil

2024

Cureus

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Background: Enterobacteriaceae are a group of aerobic and facultative anaerobic Gram-negative bacilli known to cause various infections in healthy folks and those with preexisting health conditions. Aim: Current research focuses on analyzing the bacteriological profile of clinical isolates, examining their antimicrobial susceptibility and the spectrum of drug resistance. Materials and methods: The study was conducted on patients admitted to the inpatient/outpatient department at Krishna Hospital, Karad. Clinical samples from patients with suspected infections, including pus, sputum, urine, blood, and other body fluids, were examined. The 156 bacterial isolates were identified using Gram staining and biochemical tests following the standard protocol. Results: Of the 156 isolates, Klebsiella pneumoniae was the most common isolate, with 79 (50.6%), followed by Escherichia coli , with 52 (33.3%). The maximum resistance was demonstrated toward co-trimoxazole (96.3%), ciprofloxacin (88.7%), and ceftazidime (84.6%). Conclusion: During the study, the prevalence of metallo-β-lactamase (MBL), extended-spectrum β-lactamase (ESBL), and AmpC increased significantly. The study data showed the overall prevalence of MBL, ESBL, and AmpC to be 67.3%, 37.8%, and 38.4%, respectively. Further, the rate of multidrug-resistant isolates was noteworthy (92.6%). Thus, comprehending the resistance pattern and epidemiology of the organism within a specific demographic area can help create better guidelines to curtail such infections.

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Prevalence of various Beta-lactamases in Enterobacteriaceae in a tertiary care hospital in South India: A Cross-sectional study

Cited by 3 publications

References 22 publications

Phenotypic detection of ESBL and Amp C beta lactamase among clinical isolates of Enterobacteriaceae

Phenotypic detection of ESBL and Amp C beta lactamase among clinical isolates of Enterobacteriaceae

Modified Carbapenem Inactivation Method and Ethylenediaminetetraacetic Acid (EDTA)-Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Enterobacterales and Pseudomonas aeruginosa

An Insight Into the Bacteriological Profile: Escalating Antimicrobial Resistance Patterns and the Spectrum of Multidrug-Resistant Enterobacteriaceae at a Tertiary Care Center in Western Maharashtra

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