Prevalence of Vibrio alginolyticus in Sediment Samples of River and Coastal Areas of Bangladesh

Abstract

Vibrio alginolyticus has been thought to be a halophilic marine bacterium that causes diarrhea, otitis media and wound infection through the consumption of raw or inappropriately cooked sea food. It is one of the main Vibrio pathogens affecting marine animals, such as marine fish, shrimp and shellfish which lead to large economic damage. Although there are reports on the presence of this organism in the coastal area of other countries, not so much work has been done on the isolation and characterization of this species in Bangladesh. The present study was, therefore, undertaken to isolate and characterize V. alginolyticus organisms isolated from the rivers (fresh water) and estuaries (brackish water) of Bangladesh. A total of 9 isolates of Vibrio species were obtained from different water bodies (three from Meghna river, two from Shangu river and four from estuary) and provisionally identified as Vibrio alginolyticus following standard biochemical tests. All these 9 strains showed same pattern of antibiotic resistance to ampicillin, streotomycin, penicillin, but sensitive to nalidixic acid. In the virulence properties test, two isolates showed positive results for toxR gene and none of the isolates showed positive results for tdh gene. Challenge experiments in Singhi fish (Heteropneustes fossi) with the live cells and the culture filtrate prepared from the V. alginolyticus showed high mortality of the fish population. All these studies suggest the presence of pathogenic V. alginolyticus strains in the river water and estuarine bodies of Bangladesh and the extracellular toxin(s) of the V. alginolyticus might be one of the causes for fish mortality.

Identification of a Novel Small RNA srvg23535 in Vibrio alginolyticus ZJ-T and Its Characterization With Phenotype MicroArray Technology

“…Vibrio alginolyticus , a Gram-negative halophilic bacterium with worldwide distribution, is an opportunistic pathogen of marine animals and humans, which causes serious infections, such as otitis media, otitis externa, and gastroenteritis ( Citil et al, 2015 ; Siddiqui et al, 2016 ). During invasion, pathogenic bacteria may be exposed to a variety of harsh environments, including high pH, high salinity, and starvation ( Ulitzur, 1975 ; Roop et al, 2009 ).…”

The ilvGMEDA Operon Is Regulated by Transcription Attenuation in Vibrio alginolyticus ZJ-T

“…Vibrio alginolyticus is a Gramnegative halophilic bacterium that is ubiquitous in marine and estuarine environments. It is an opportunistic pathogen responsible for vibrioses in fish and shellfish and causes skin and ear infections in humans (16,17).…”

Screening of Marine Bacterium Vibrio Alginolyticus Strain As05 for the Production of N-Acyl Homoserine Lactone-Based Quorum Sensing Signaling Molecules

“…Vibrio alginolyticus is a Gram-negative, motile, nonsporing, curved rod-shaped bacterium associated with the family of Vibrionaceae of Gamma-proteobacteria. Previously, based on similar genotypic characteristics, V. alginolyticus was considered a biotype of V. parahemolyticus (Siddiqui et al, 2012). However, these two species can be differentiated based on the fermentation of sucrose carried out by V. alginolyticus.…”