Prevalence of virulence-associated genes and cytolethal distending toxin production in Campylobacter spp. isolated in Italy

Ripabelli, Giancarlo; Tamburro, Manuela; Minelli, Fabio; Leone, Annalisa de; Sammarco, Michela Lucia

doi:10.1016/j.cimid.2008.12.001

Cited by 45 publications

(36 citation statements)

References 23 publications

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“…These results are similar to data previously reported by other authors (Datta et al 2003;Rozynek et al 2005;Krutkiewicz and Klimuszko 2010;Rapabelli et al 2010). The invasion-associated marker (iam) of Campylobacter was another virulence marker detected in this study.…”

Section: Discussionsupporting

confidence: 83%

“…However, several studies showed that certain bacterial factors are essential for the pathogenesis of campylobacteriosis, including the motility and adherence of bacteria to intestinal mucosa, capability to invade enterocytes as well as toxin production (Datta et al 2003;Dasti et al 2010). Moreover, some potential genetic markers of bacterial virulence have been identified such as flaA and cadF involved in adhesion and colonisation, virB11 and iam associated with invasiveness, as well as the cdtA, cdtB and cdtC toxin genes encoding Campylobacter cytotoxins Dasti et al 2010;Rapabelli et al 2010).…”

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confidence: 99%

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Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Wieczorek¹,

Szewczyk²,

Osek³

2012

Vet. Med.

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ABSTRACT:The study was conducted to investigate the presence of Campylobacter spp. in meat sold to consumers at a retail market in Poland. Antimicrobial resistance and the presence of putative virulence genes of the isolates were also examined. A total of 558 meat samples, including beef (n = 105), pork (n = 85), and poultry (n = 368) were collected over an almost three year study period. It was found that 321 samples, all of them originating from poultry, were contaminated with Campylobacter spp. Most of the obtained isolates were classified as C. coli (189 strains, 58.9%), whereas C. jejuni was identified in 132 (41.1%) samples. All Campylobacter strains were susceptible to gentamicin and all but one C. coli isolate to erythromycin. On the other hand, the highest level of resistance among Campylobacter tested was to ciprofloxacin (91% for C. jejuni and 86.1% for C. coli) and nalidixic acid (89.3% for C. jejuni and 85% for C. coli). Furthermore, resistance to two or more classes of antibiotics was found in the majority (60.9%) of Campylobacter spp. and among them one C. coli strain showed resistance to four different classes of antimicrobials. Identification of virulence genes in the isolated Campylobacter showed that all of them had the flaA and cadF genes. The iam marker was found more often in C. coli strains (88.8%) compared to C. jejuni isolates (53.8%). On the other hand, the virB11 gene was identified only in 4.2% of C. coli and in 6.1% of C. jejuni strains, respectively. Furthermore, the prevalence of the cdtA, cdtB, and cdtC genes among C. jejuni strains was 97.7%, 93.2%, 96.2%, respectively, and was significantly higher than for C. coli regarding the cdtC (66.7%) gene. The obtained results showed that the presence of Campylobacter in retail meat may represent a threat to public health.

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Section: Discussionsupporting

confidence: 83%

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confidence: 99%

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Wieczorek¹,

Szewczyk²,

Osek³

2012

Vet. Med.

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“…In accordance with our findings, other studies regarding the prevalence of VAGs worldwide also found a very high proportion of these genes in different types of clinical and animal samples (Datta et al, 2003;Al-Mahmeed et al, 2006;Talukder et al, 2008;Ripabelli et al, 2010;Andrzejewska et al, 2011;Hamidian et al, 2011), especially ciaB (95-98 %) and the cdtABC operon (85-100 %).…”

Section: Information From Elisa Testssupporting

confidence: 80%

Campylobacter jejuni infection and virulence-associated genes in children with moderate to severe diarrhoea admitted to emergency rooms in northeastern Brazil

Quetz

Lima

Havt

et al. 2012

Journal of Medical Microbiology

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Campylobacter is an important cause of foodborne gastroenteritis. We determined the occurrence of Campylobacter jejuni and Campylobacter coli, using culture-based methods and PCRs targeting virulence-associated genes (VAGs) among children aged ¡14 years who were treated for diarrhoea at emergency rooms in northeastern Brazil. Genomic DNA was extracted directly from stool samples collected from 366 children. A questionnaire was also applied to qualify the clinical conditions presented by each child at the time of admission. C. jejuni and C. coli were detected in 16.4 % (60/366) and 1.4 % (5/366) of the diarrhoeal samples, respectively, by PCR, a much higher proportion than that detected by conventional methods. C. jejuni VAGs were detected in the following proportions of hipO-positive samples: ciaB, 95 % (57/60); dnaJ, 86.7 % (52/60); racR, 98.3 % (59/60); flaA, 80 % (48/60); pldA, 45 % (27/60); cdtABC, 95 % (57/60); and pVir 0 % (0/ 60). Particular symptoms, such as blood in faeces, vomiting, fever, and/or abdominal pain, were not associated with detection of C. jejuni nor were they associated with any particular VAG or combination of VAGs (P.0.05). C. jejuni and its VAGs were detected in a substantial proportion of the children admitted. Further efforts shall be directed towards elucidating whether these genetic factors or their expressed proteins play a role in Campylobacter pathogenesis. INTRODUCTIONThe genus Campylobacter, a group of thermotolerant, microaerophilic, Gram-negative bacteria, includes a number of pathogens that primarily cause gastrointestinal disease in humans, particularly Campylobacter jejuni and Campylobacter coli. Campylobacter-associated gastroenteritis is thought to occur through zoonotic transmission, being acquired from exposure to tainted food and/or contaminated drinking water (Sherman et al., 2010). Campylobacter infection frequently presents as self-limiting acute enteritis with diarrhoea, malaise, fever and abdominal pain, sometimes with vomiting and the presence of blood in faeces (Allos, 2001); disruption of epithelial cells and inflammation of the intestinal mucosa are hallmark features of severe cases (Beltinger et al., 2008). C. jejuni and C. coli cause significant morbidity worldwide, especially in children (Amieva, 2005;Tam et al., 2003;Wang et al., 2008; Fernández et al., 2008).Adherence and colonization are crucial steps in the pathogenesis of C. jejuni. Flagella have a major role in invasion; markedly reduced internalization in vitro has been reported with flaA 2 C. jejuni mutants (Wassenaar, 1997). The genes racR and dnaJ are determinants for C. jejuni colonization and are presumably expressed in response to conditions encountered in the intestinal microenviroment, such as differences in temperature between environmental reservoirs and human intestines (Konkel et al., 1998; Brás et al., 1999 antigen B protein, which confers invasive properties, as shown by C. jejuni ciaB null mutants which display a significant reduction in internalization (Konkel et al., 1999). Also, ...

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“…CDT is a bacterial protein toxin that is widely distributed among many Gram-negative bacteria including E. coli strains of many serotypes and some strains belonging to Shigella dysenteriae, Shigella boydii, and various species of Campylobacter [2,3,16,28]. Previously, Park and Richardson [25] revealed that CDT was able to stop proliferation of cell lines.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of virulence and cytolethal distending toxin (CDT) genes in thermophilic Campylobacter spp. from dogs and humans in Gyeongnam and Busan, Korea

Cho¹,

Kim²,

Min³

et al. 2014

Korean Journal of Veterinary Research

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: The prevalence of thermophilic Campylobacter (C.) spp. in stray, breeding, and household dogs was 25.2, 12.0, and 8.8%, respectively. C. jejuni and C. upsaliensis were the predominant Campylobacter spp. from household dogs. cdtA, cdtB, and cdtC were detected by PCR in all isolates. Despite the high cytolethal distending toxin (CDT) gene prevalence, only 26 (31%) C. jejuni strains and one (15.3%) C. coli strain showed evidence of CDT production in HEp-2 cell cytotoxicity assays. Virulence-associated genes detected in the C. jejuni and C. coli isolates were cadF, dnaJ, flaA, racR, ciaB, iamA, pldA, virB11, ceuE, and docC. cadF, dnaJ, flaA, and ceuE were found in all C. jejuni and C. coli isolates. When detecting Guillain-Barré syndrome-associated genes (galE, cgtB, and wlaN), galE was identified in all isolates. However, cgtB and wlaN were more prevalent in C. jejuni isolates from humans than those from dogs. Adherence and invasion abilities of the C. jejuni and C. coli strains were tested in INT-407 cells. A considerable correlation (adjusted R 2 = 0.678) existed between adherence and invasion activities of the Campylobacter spp. isolates.

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Prevalence of virulence-associated genes and cytolethal distending toxin production in Campylobacter spp. isolated in Italy

Cited by 45 publications

References 23 publications

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Campylobacter jejuni infection and virulence-associated genes in children with moderate to severe diarrhoea admitted to emergency rooms in northeastern Brazil

Prevalence of virulence and cytolethal distending toxin (CDT) genes in thermophilic Campylobacter spp. from dogs and humans in Gyeongnam and Busan, Korea

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