Prevalence of virulent Candida spp. in complicated urinary tract infection of nephrolithiatic patients from surgical units of tertiary care hospitals Islamabad

Zafar, Sahar F.; Fatima, Kaneez; Faryal, Rani

doi:10.1016/j.mycmed.2020.101024

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…These differences suggest that the direct detection of lipase activities on agar plates (this study) can provide higher rates of detection. As to our results obtained at pH 6.5 with blood (75%), vaginal (50%), and urine (25%) isolates, they were consistent with the high occurrence of lipase‐producing strains among C. albicans strains associated with blood (56.2%; Pandey et al, 2018 ) and differed from those (86.2%) obtained by Zafar et al ( 2020 ) for urine isolates. Thus, our data indicate that pH is an important factor in determining the production of lipase‐like enzymes by C. albicans .…”

Section: Discussionsupporting

confidence: 91%

Assessing pH‐dependent activities of virulence factors secreted by Candida albicans

et al. 2023

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Candida albicans is an opportunistic pathogen that can thrive under adverse conditions including suboptimal pH, nutrient scarcity, and low levels of oxygen. Its pathogenicity is associated with the production of virulence factors such as extracellular hydrolytic enzymes and toxins. This study was aimed at determining the effect of external pH, substrate nature, and strain origin on protease, lipase, and hemolysin production. To achieve this objective, agar plate assays were performed at pH 5.0, 6.5, and 7.5 with substrates suitable for the detection of each family of enzymes. Moreover, the study was conducted with 20 clinical C. albicans isolates from blood, oral cavity, skin, urine, and vagina. The hydrolytic zones formed around the colonies were further measured to calculate the Ez (enzymatic zone) indexes. We found that detection of proteases in skim milk agar plates was possible for most isolates only at pH 5 (80%) and pH 6.5 (75%), whereas BSA plates could confer protease detection exclusively at pH 5 (80%). Similarly, the percentage of isolates possessing lipolytic activities was higher at pH 5 (90%) than at pH 6.5 (70%) and pH 7.5 (35%). In contrast, hemolytic activities were detected in all isolates at pH 6.5 and 7.5 but not at pH 5. Further analysis revealed that some differences in the detected activities could potentially be attributed to the anatomical origin of these isolates. Collectively, these findings suggest that the pH of the site of infection might be critical for mimicking the microenvironment employed to experimentally discover the key virulence factors.

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Section: Discussionsupporting

confidence: 91%

Assessing pH‐dependent activities of virulence factors secreted by Candida albicans

et al. 2023

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“…Candidiasis frequently affects human skin, oral and esophageal areas, the gastrointestinal tract, and the vagina. While it primarily occurs in those with compromised immune systems or other debilitations, Candida albicans, the primary causative agent, exhibits multiple virulence factors that aid in its pathogenicity [ 1 , 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light

Wiench,

Paliga,

Mertas

et al. 2024

Dentistry Journal

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Background: Candida albicans and similar species are significant pathogens in immunocompromised and hospitalized individuals, known for mucosal colonization and bloodstream/organ invasion. Many pathogenic fungi, including these species, exhibit autofluorescence (R/OF) under specific light conditions, a feature crucial for their detection. Aim: We investigated the use of a 405 nm diode laser for the direct observation of red/orange autofluorescence of Candida spp., common in the oral cavity, exploring its potential in health screenings. Methods: This study utilized cultures of Candida spp. on Sabouraud dextrose agar with Qdot 655 and 685 for fluorescence benchmarking, illuminated using a 405 nm diode laser (continuous wave, power 250 mW, 0.0425 J/cm² fluence, 0.0014 W/cm² power density). Images were captured using a yellow-filter camera at set intervals (48 to 144 h). Visual and computational analyses evaluated the R/OF in terms of presence, intensity, coloration, and intra-colony variation. Results: Most Candida strains displayed red/orange autofluorescence at all observation times, characterized by varied coloration and intra-colony distribution. Initially, there was an increase in R/OF intensity, which then stabilized in the later stages of observation. Conclusions: The majority of the Candida strains tested are capable of emitting R/OF under 405 nm laser light. This finding opens up new possibilities for integrating R/OF detection into routine dental screenings for Candida spp.

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Application of natural products against fungal biofilm formation

Samreen

Jafri

Ahmad

2022

A Complete Guidebook on Biofilm Study

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Prevalence of virulent Candida spp. in complicated urinary tract infection of nephrolithiatic patients from surgical units of tertiary care hospitals Islamabad

Cited by 3 publications

References 17 publications

Assessing pH‐dependent activities of virulence factors secreted by Candida albicans

Assessing pH‐dependent activities of virulence factors secreted by Candida albicans

Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light

Application of natural products against fungal biofilm formation

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