Prevalence of Yersinia enterocolitica in Pigs Slaughtered in Chinese Abattoirs

Liang, Junrong; Wang, Xin; Xiao, Yuchun; Cui, Zhigang; Xia, Shengli; Qin, Hao; Yang, Jinchuan; Luo, Longze; Wang, Shukun; Li, Kewei; Yang, Haoshu; Gu, Wenpeng; Xu, Jianguo; Kan, Biao; Jing, Huaiqi

doi:10.1128/aem.07893-11

Cited by 43 publications

(43 citation statements)

References 30 publications

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“…Macrorestriction analysis was performed to investigate the clonality of the isolates. NotI (Roche Diagnostics, Mannheim, Germany)-digested fragments of genomic DNA were separated in a CHEF DR II system (Bio-Rad, Munich, Germany) as described by Liang and coworkers (30). The total run time was 19 h, and 0.5ϫ Tris-borate-EDTA buffer was used as the running buffer.…”

Section: Species Identification By Maldi-tof Ms and Cultivation Of Ismentioning

confidence: 99%

Yersinia enterocolitica Isolates from Wild Boars Hunted in Lower Saxony, Germany

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Kehrenberg

2015

Appl Environ Microbiol

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Yersiniosis is strongly associated with the consumption of pork contaminated with enteropathogenic Yersinia enterocolitica, which is harbored by domestic pigs without showing clinical signs of disease. In contrast to data on Y. enterocolitica isolated from conventionally reared swine, investigations into the occurrence of Y. enterocolitica in wild boars in Germany are rare. The objectives of the study were to get knowledge about these bacteria and their occurrence in wild boars hunted in northern Germany by isolation of the bacteria from the tonsils, identification of the bioserotypes, determination of selected virulence factors, macrorestriction analysis, multilocus sequence typing (MLST), and testing of antimicrobial susceptibility. Altogether, tonsils from 17.1% of 111 tested wild boars were positive for Y. enterocolitica by culture methods. All but two isolates belonged to biotype (BT) 1A, with the majority of isolates bearing a ystB nucleotide sequence which was revealed to have 85% identity to internal regions of Y. enterocolitica heat-stable enterotoxin type B genes. The remaining Y. enterocolitica isolates were identified to be BT 1B and did not carry the virulence plasmid. However, two BT 1A isolates carried the ail gene. Macrorestriction analysis and results from MLST showed a high degree of genetic diversity of the isolates, although the region where the samples were taken was restricted to Lower Saxony, Germany, and wild boars were shot during one hunting season. In conclusion, most Y. enterocolitica isolates from wild boars investigated in this study belonged to biotype 1A. Enteropathogenic Y. enterocolitica bioserotypes 4/O:3 and 2/O:9, usually harbored by commercially raised pigs in Europe, could not be identified.

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Section: Species Identification By Maldi-tof Ms and Cultivation Of Ismentioning

confidence: 99%

Yersinia enterocolitica Isolates from Wild Boars Hunted in Lower Saxony, Germany

Altrock

Seinige

Kehrenberg

2015

Appl Environ Microbiol

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“…Y. pseudotuberculosis and Y. enterocolitica are zoonotic food-borne pathogens that spread through the fecal-oral route; they have a broad host range, infecting animals, including swine, dogs, rodents, birds, and wild animals (3,4). Many studies show that swine and dogs are the most common sources of Y. enterocolitica infections in humans (5,6). These enteropathogens cause human enteric diseases, both sporadically and in epidemics worldwide.…”

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confidence: 99%

Homology Analysis of Pathogenic Yersinia Species Yersinia enterocolitica, Yersinia pseudotuberculosis, and Yersinia pestis Based on Multilocus Sequence Typing

et al. 2014

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“…Additionally, the important virulence genes are usually missing in animal strains. Human pathogenic strains of Y. enterocolitica typically have been isolated from slaughtered fattening pigs (Fredriksson-Ahomaa et al 2007b;Ortiz Martínez et al 2010;Vanantwerpen et al 2013;Liang et al 2012). Recently, it was shown that there can be a large variation in the within-batch prevalence among pig farms (Vanantwerpen et al 2013).…”

Section: Prevalence In Animalsmentioning

confidence: 99%

Enteropathogenic Yersinia spp.

Fredriksson-Ahomaa

2014

Zoonoses - Infections Affecting Humans and Animals

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Yersinia enterocolitica and Yersinia pseudotuberculosis can cause enteric yersiniosis among humans and animals. The infection is typically acquired through contaminated food but it can also be transmitted directly through human or animal contact. Common symptoms are diarrhoea, abdominal pain and fever but sometimes sequelae as joint pain and skin rash occur. The severity of the infection depends on the age and immunity of the infected person, the virulence of the strain and the infection dose. Y. enterocolitica and Y. pseudotuberculosis differ clearly from each other both pheno-and genotypically. Y. enterocolitica species consists of a very heterogeneous group of bacteria and not all Y. enterocolitica strains are pathogenic. Y. pseudotuberculosis strains show little variation in biochemical reactions and they are all considered to be pathogenic. Both plasmid and chromosomal encoded virulence factors are needed for Yersinia pathogenicity and all pathogenic strains carry a virulence plasmid which essential for the bacteria to multiply and disseminate in the host. Yersinia strains can grow at low temperatures and under both aerobic and anaerobic conditions but they are heat sensitive. The isolation and identification of enteropathogenic Yersinia is difficult and time consuming, especially when food and environmental samples are studied. More accurate methods should be designed for isolation and identification of Yersinia spp. and more information is needed about transmission routes and sources of enteropathogenic Yersinia strains.

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Prevalence of Yersinia enterocolitica in Pigs Slaughtered in Chinese Abattoirs

Cited by 43 publications

References 30 publications

Yersinia enterocolitica Isolates from Wild Boars Hunted in Lower Saxony, Germany

Yersinia enterocolitica Isolates from Wild Boars Hunted in Lower Saxony, Germany

Homology Analysis of Pathogenic Yersinia Species Yersinia enterocolitica, Yersinia pseudotuberculosis, and Yersinia pestis Based on Multilocus Sequence Typing

Enteropathogenic Yersinia spp.

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