Prevalence, phenotypic and genotypic diversity, antibiotic resistance, and frequency of virulence genes in Pseudomonas aeruginosa isolated from shrimps

Dehkordi, Seyed Majid Hashemi; Anvar, Amir Ali; Rahimi, Ebrahim; Ahari, Hamed; Ataee, Maryam

doi:10.1007/s10499-021-00798-z

Cited by 1 publication

(1 citation statement)

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“…Further, bla DHA-1 was detected in Enterobacterales, such as Proteus mirabilis , E. coli and K. pneuomoniae , collected from pig production in India and Korea [ 12 , 27 ]. Food of animal origin can also be considered a source of bla DHA-1 as it has been described in a number of publications in recent years, especially in Pseudomonas aeruginosa collected from bovine and ovine meat, raw milk and fishery products from Iran [ 28 , 29 , 30 , 31 ] but also in K. pneumoniae and E. coli strains isolated from shrimp in Germany, in E. coli isolated from beef products in South Africa and in K. pneumoniae isolated from ready-to-eat sandwiches in Algeria [ 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Rare Plasmid-Mediated AmpC Beta-Lactamase DHA-1 Located on Easy Mobilized IS26-Related Genetic Element Detected in Escherichia coli from Livestock and Food in Germany

Manfreda,

Kaesbohrer,

Schmoger

et al. 2024

Microorganisms

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AmpC beta-lactamases cause resistance to third-generation cephalosporins, including beta-lactamase inhibitors. In Escherichia coli from the German food production chain, the majority of AmpC beta-lactamase activity can be attributed to plasmid-mediated CMY-2 or overproduction of chromosomal AmpC beta-lactamase, but occasionally other enzymes like DHA-1 are involved. This study investigated the prevalence of the AmpC beta-lactamase DHA-1 in ESBL/AmpC-producing E. coli (n = 4706) collected between 2016 and 2021 as part of a German antimicrobial resistance monitoring program along the food chain. Eight isolates (prevalence < 0.2%) were detected and further characterized by PFGE, transformation and conjugation experiments as well as short-read and long-read sequencing. All eight strains harbored blaDHA-1 together with qnrB4, sul1 and mph(A) resistance genes on an IS26 composite transposon on self-transferable IncFII or IncFIA/FIB/II plasmids. During laboratory experiments, activation of the translocatable unit of IS26-bound structures was observed. This was shown by the variability of plasmid sizes in original isolates, transconjugants or transferred plasmids, and correspondingly, duplications of resistance fragments were found in long-read sequencing. This activation could be artificial due to laboratory handling or naturally occurring. Nevertheless, DHA-1 is a rare AmpC beta-lactamase in livestock and food in Germany, and its dissemination will be monitored in the future.

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