Prevalence, serotype and antibiotic susceptibility of Group B Streptococcus isolated from pregnant women in Jakarta, Indonesia

Abstract

Group B Streptococcus (GBS) is a bacterial pathogen which is a leading cause of neonatal infection. Currently, there are limited GBS data available from the Indonesian population. In this study, GBS colonization, serotype distribution and antimicrobial susceptibility profile of isolates were investigated among pregnant women in Jakarta, Indonesia. Demographics data, clinical characteristics and vaginal swabs were collected from 177 pregnant women (mean aged: 28.7 years old) at 29–40 weeks of gestation. Bacterial culture identification tests and latex agglutination were performed for GBS. Serotyping was done by conventional multiplex PCR and antibiotic susceptibility testing by broth microdilution. GBS colonization was found in 53 (30%) pregnant women. Serotype II was the most common serotype (30%) followed by serotype III (23%), Ia and IV (13% each), VI (8%), Ib and V (6% each), and one non-typeable strain. All isolates were susceptible to vancomycin, penicillin, ampicillin, cefotaxime, daptomycin and linezolid. The majority of GBS were resistant to tetracycline (89%) followed by clindamycin (21%), erythromycin (19%), and levofloxacin (6%). The serotype III was more resistant to erythromycin, clindamycin, and levofloxacin and these isolates were more likely to be multidrug resistant (6 out of 10) compared to other serotypes. This report provides demographics of GBS colonization and isolate characterization in pregnant women in Indonesia. The results may facilitate preventive strategies to reduce neonatal GBS infection and improve its treatment.

Clinical Profile and Risk Factors of Group B Streptococcal Colonization in Mothers from the Eastern District of China

“…Further, logistic regression was conducted to validate the relationships between maternal age, gestational age of birth, PROM, and GBS colonization. Consistent with some previous reports, older age was associated with greater susceptibility to GBS carriage [OR = 1.913(1.662, 2.478)] [ 11 , 30 ]. In contrast, increasing age was significantly linked to lower rates of colonization in other research [ 31 , 32 ].…”

“…We observed a greater likelihood of lower gestational age at birth for GBS-colonized mothers (OR � 1.992 (1.445, 2.746)). Moreover, consistent with data obtained from Inner Mongolia, but not Xiamen and Jakarta, GBS-colonized mothers were more likely to undergo PROM (OR � 3.838 (1.619, 9.099)) [11,18,30].…”

“…e lack of association of parity, gravidity, delivery method, birth [11,30]. In contrast, increasing age was significantly linked to lower rates of colonization in other research [31,32].…”

“…Serotyping of GBS isolates was conducted via conventional multiplex PCR. The primers were designed according to the reference sequences and synthesized by An Hui General Biosystems (Chuzhou, China) [ 10 , 11 ].…”

A Retrospective National Study on Colonization Rate and Antimicrobial Susceptibility of Streptococcus agalactiae in Pregnant Korean Women, 2018–2020

“…13 Therefore, inducible clindamycin resistance detection should be performed in women with high beta-lactam antibiotic anaphylaxis risk. The multidrug-resistant GBS ratio of 32.2% obtained in this study was higher than the 18.9% reported by Safari, et al 26 and lower than the 35.2% reported by Leykun, et al 25 Most of the clindamycin-resistant GBS strains were also resistant to erythromycin and tetracycline. Accordingly, documentation on the detection of clindamycin resistance, including inducible resistance, should be included in the Korean guidelines for the prevention of perinatal GBS infection.…”