Prevalência de anticorpos contra Yersinia pestis em carnívoros domésticos nos focos pestosos, do Estado do Ceará

Abstract

A peste é primordialmente uma zoonose focal de roedores silvestres transmitida por pulgas que infectam outros mamíferos: lagomorfos (coelhos e lebres), primatas não humanos, alguns insetívoros (musaranho e porco-espinho), camelos, carnívoros selvagens (raposas e gambás) e domésticos (cães e gatos). o homem infecta-se no seu próprio domicílio ou quando se expõe ao ABSTRACTThe prevalence of antibodies against Yersinia pestis in domestic carnivores (dogs and cats), in plague areas in the State of Ceará, was analyzed to establish the importance of monitoring these animals within the routine practice of the plague control program. over the decade 1997-2006, 146,732 serum samples were examined (95,883 from dogs and 50,849 from cats), of which 2,629 (2,234 from dogs and 395 from cats) proved to be positive. The prevalence among dogs (85%) was higher than among cats (15%) throughout the decade and in all places, except in ibiapina in 1998. The significance of these findings has not yet been determined. Studies on this zoonosis in Brazil have been based on paradigms that did not cover all the elements involved in the zoonosis, thus making it impossible to properly understand the role of these carnivores. Monitoring of plague foci conducted exclusively by means of dog surveys may result in progressive lack of knowledge of the epidemiological situation of plague, if supplementary inter-institutional research is not developed. .os cães e gatos, carnívoros domésticos, predadores dos roedores, infectam-se por picadas de pulgas ou consumindo tecidos infectados de roedores. Desenvolvem, então, anticorpos que permanecem detectáveis por vários meses, o que justifica a sua utilização como animais sentinela/detectores das atividades da infecção entre os roedores/reservatórios nas áreas focais 8 11 16 . o monitoramento da circulação do patógeno e a proteção das populações humanas

Seroprevalence and spatial distribution dynamics of Yersinia pestis antibodies in dogs and cats from plague foci in the State of Ceará, Northeastern Brazil

“…Previous studies found that antibodypositivity prevalence among dogs and cats from the Ceará plague foci, and from Chapada da Borborema (PB), were similar 13,22 . During a plague outbreak in a village in Yunnan Province, China serum-positivity rates in domestic cats and dogs were 23.5% and 26.5%, respectively 23 .…”

“…The field surveillance program was conducted during annual cycles of health agent visits and blood sample collections from dogs and cats for serological analyses 13 .…”

“…Passive Hemagglutination Assays/HemagglutinationInhibition (HA/HI) assays were used to detect specific Y. pestis anti-F1 antibodies 13 . In brief, the tests were performed using 96-well U-shaped polystyrene plates.…”

“…A titer of >1/16 was considered antibody-positive. The NRS used to prepare the buffers and all sera tests were previously inactivated at 56°C for 30 min in a water-bath and adsorbed for 30 min at room temperature with fresh sheep red blood cells 13 .…”

“…Titers >1/64 suggest recent infections and recent contact with Y. pestis, signaling a potential risk for human infection [12][13]22 . Paul-Murphy et al 31 reported anti-Y.…”