The research aimed to explore the incidence of Chlamydia trachomatis infection among adolescents attending the Nuevo San Rafael Health Center, focusing on identifying risk factors and assessing the effectiveness of educational strategies. The study utilized a mixed-methods approach combining qualitative and quantitative data, with a sample size of 180 adolescents selected from a population of 350. Data collection involved surveys and statistical analysis to establish correlations between risk factors and infection rates. The results indicated that a significant percentage of adolescents lacked proper knowledge about sexual health and safe practices, with 77% not knowing how to effectively use condoms and 52% never attending sexual health check-ups. These gaps in knowledge and healthcare engagement are critical, as Chlamydia trachomatis is a frequently asymptomatic infection that can lead to severe complications if untreated. The study also highlighted the prevalence of risky behaviors, including multiple sexual partners and substance use during sexual activities, which are significant contributors to the spread of this infection. The research concluded that there is an urgent need for targeted educational interventions to improve sexual health awareness and preventive practices among adolescents. The findings underscore the importance of early education and regular health check-ups in preventing the spread of Chlamydia trachomatis and other sexually transmitted infections, advocating for the implementation of comprehensive sexual education programs in schools and healthcare facilities